Display omitted •Endogenous NO levels are higher in roots of Al-tolerant wheat.•NO precursors decrease Al accumulation in wheat root cells.•NO precursors stimulate auxin flow towards roots in Al-treated wheat.•Al-induced oxidative stress is attenuated in wheat roots by NO precursors.•Increased endogenous NO content contribute, in part, to wheat Al tolerance. Aluminum (Al) is an element widely distributed in soils, even though Al3+ is one of the most detrimental cations to plant growth. The effect of nitric oxide (NO) precursors on indole-3-acetic acid (IAA) flow towards roots upon Al treatment is herein reported using two Triticum aestivum (wheat) cultivars with recognized differential Al tolerance. Roots of Al-tolerant seedlings with no treatment (control) accumulated higher amounts of NO than Al-sensitive ones. The treatment with Al further stimulated NO production in root cells while root exposure to NO3−, L-arginine (Arg) or the NO donor S-nitrosoglutathione (GSNO) decreased both Al and lipid peroxide accumulation in both cultivars. Regardless of the cultivar, NO3−, Arg or GSNO prevented the blockage of IAA flow towards roots. Overall, the treatment of wheat roots with NO precursors prior to Al treatment effectively guarantees normal IAA flow towards roots, a condition that favors the organ’s growth and development.