Phytochemical investigation of Lindera myrrha rootsgrowing in Vietnam afforded a new eudesmane sesquiterpenoid, myrrhalindenane C (1), along with seven known compounds, rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo3,2,1-oct-8-yl)-3-methyl-2Z,4E-pentadienoic acid (2), 1-O-(4-hydroxy-2,6-dimethoxyphenoxy)-6-O-rel-5-(3S,8S-dihydroxy-1R,5S-dimethyl-7-oxa-6-oxobicyclo3,2,1-oct-8-yl)-3-methyl-2Z,4E-pentadienoyl-β-D-glucopyranose (3), curcumin (4), demethoxycurcumin (5), bisdemethoxycurcumin (6), (1E,6E)-1,7-bis(4-methoxyphenyl)-1,6-heptadiene-3,5-dione (7), and 2',4',4,2''-tetrahydroxy-3'-3''-methylbut-3''-enylchalcone (8). The structure was determined by analysis of their MS and NMR data as well as by comparison with literature values. All compounds were evaluated for antimicrobial activity against antibiotic-resistant, pathogenic bacteria Enterococcus faecium, Staphylococcus aureus, and Acinetobacter baumannii.