The purpose of this study was to investigate the influence of large-scale circulation on the flow field in a cabin mockup. The velocity was measured by ultrasonic anemometers (UA). Then, this study analyzed the turbulence kinetic energy spectra of the velocity fluctuation signal. The turbulence kinetic energy spectra of the measurement points reflect the flow characteristic of the large-scale circulation in the cabin mockup. The results contribute to the understanding of the role of the thermal plume on the large-scale circulation in the cabin. The large-scale circulation's impact on air quality was also investigated, and the contaminant distribution was measured using tracer gas in the cabin. The two large-scale circulation interactions made the air flow mixing approximately uniform. •The turbulence kinetic energy spectrum analysis method can reveal the effect of thermal plumes on large-scale circulation.•The thermal plume transmitted energy to the large-scale circulation through the process of circulation entrainment.•The two large-scale circulation interactions made the air flow mixing approximately uniform.