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Baum, Jeramy; Jones, Riland; Manning, Thomas; Nienow, James; Phillips, Dennis
Acta pharmaceutica (Zagreb, Croatia), 06/2012, Volume: 62, Issue: 2Journal Article
Laser light scattering and scanning electron microscopy (SEM) are used to study hemoglobin in the aqueous phase. The impact that salts NaCl, Ca3(PO4)2 and iron oxide nanoparticles have on the hemoglobin size are also studied. The first set of experiments examined hemoglobin aggregates in the aqueous phases in the presence of salts and nanoparticles. Aqueous phase samples were then dehydrated and examined using SEM. The resulting structures resemble those observed in nanobacteria studies conducted in other labs. This study demonstrates that aggregates of hemoglobin and various salts found in a physiological environment can produce structures that resemble nanobacteria. Metoda dinamičkog rasapa svjetlosti i pretražna elektronska mikroskopija (SEM) koriš tene su za proučavanje hemoglobina u vodenoj fazi. Nadalje, ispitivan je utjecaj soli NaCl, Ca3(PO4)2 i nanočestica oksida željeza na veličinu hemoglobina. U prvom setu pokusa proučavani su agregati hemoglobina u vodenoj fazi u prisutnosti soli i nanočestica. Vodena faza je dehidrirana i ostatak analiziran pomoću pretražne elektronske mikroskopije. Agregati hemoglobina i različitih soli pronađeni u fiziološkim uvjetima čine strukture slične nanobakterijama.
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