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Criscuolo, Alexis; Brisse, Sylvain
Genomics (San Diego, Calif.), 11/2013, Volume: 102, Issue: 5-6Journal Article
Contaminant oligonucleotide sequences such as primers and adapters can occur in both ends of high-throughput sequencing (HTS) reads. AlienTrimmer was developed in order to detect and remove such contaminants. Based on the decomposition of specified alien nucleotide sequences into k-mers, AlienTrimmer is able to determine whether such alien k-mers are occurring in one or in both read ends by using a simple polynomial algorithm. Therefore, AlienTrimmer can process typical HTS single- or paired-end files with millions of reads in several minutes with very low computer resources. Based on the analysis of both simulated and real-case Illumina®, 454™ and Ion Torrent™ read data, we show that AlienTrimmer performs with excellent accuracy and speed in comparison with other trimming tools. The program is freely available at ftp://ftp.pasteur.fr/pub/gensoft/projects/AlienTrimmer/. •Removal of alien sequences (adapters, primers) from raw reads improves the quality of results from downstream analyses.•AlienTrimmer allows detecting and removing multiple alien sequences in both ends of sequence reads.•AlienTrimmer performs accurately and has fast running time.
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