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Zhu, Dongjie; Wang, Xiangxi; Fang, Qianglin; Van Etten, James L; Rossmann, Michael G; Rao, Zihe; Zhang, Xinzheng
Nature communications, 04/2018, Volume: 9, Issue: 1Journal Article
The Ewald sphere effect is generally neglected when using the Central Projection Theorem for cryo electron microscopy single-particle reconstructions. This can reduce the resolution of a reconstruction. Here we estimate the attainable resolution and report a "block-based" reconstruction method for extending the resolution limit. We find the Ewald sphere effect limits the resolution of large objects, especially large viruses. After processing two real datasets of large viruses, we show that our procedure can extend the resolution for both datasets and can accommodate the flexibility associated with large protein complexes.
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