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  • Jovanović Snežana

    04/2013
    Dissertation

    Provider: - Institution: - Data provided by Europeana Collections- The objective of the study was to determine stability of yield and yield components in twelve maize inbred lines with different types of cytoplasm (cms-C, cms-S and fertile). The starting point was the assumption that these maize inbred lines have different genetic basis and react differently to environmental factors, affecting stability of yield and grain yield components. A trial with maize inbred lines was set up at two localities during 2008 and 2009. A 4-factorial analysis of variance (genotype-cytoplasm-year-locality) was carried out for the following properties: ear height, plant height, yield, ear length, number of grain rows, number of grains per row, grain depth, 1000-grain mass, middle round grain fraction, middle large flat fraction and middle large round fraction. Correlation bounds between the studied properties of the inbred lines were analyzed by applying the multiple correlation and regression equation. A stability assessment was done (Hühn, 1990), as well as a cluster analysis for each cytoplasmic source (Rohlf, 2000). The analysis of variance determined there were very significant differences among inbred lines for all the properties and inbred lines interactions with the studied factors (cytoplasmic type, year and locality). The inbred line L1 achieved the highest (3.3 t ha-1), and the inbred line L10 (1.5 t ha-1) the lowest grain yield. A cytoplasmic type significantly affects the studied properties of the inbred lines. Depending of the cytoplasmic type (cms-C, cms-S and fertile), fluctuation in grain yield of inbred lines was very significant in the inbred lines L2, L5, L7, L10 and L12. The average grain yield fluctuated very significantly (P≤1%) depending of the year and the localities. In 2008, the yield (2.9 t ha-1) was higher than in 2009 (2.12 t ha-1). The first locality had higher yield (2.72 t ha-1) than the second one (2.31 t ha-1). Correlation bounds in the inbred lines with different cytoplasmic types were different regarding the following properties: ear height with grain depth, 1000-grain mass and middle large flat fraction; plant height with ear length, middle large flat fraction and middle large round fraction; ear length with number of grain rows, number of grains per row and middle large round fraction; number of grain rows with middle large flat fraction and yield; number of grains per row with grain depth; grain depth with middle round grain fraction; and 1000-grain mass with middle round and middle large round grain fraction. As for the rest of the properties, i.e. their mutual correlations, no differences were found among all three cytoplasmic sources. The inbred line L10 with C cytoplasmic source expressed the highest and L12 the lowest grain yield stability by the years and the localities. When it comes to S-type stability inbred lines, L12 had the highest and L10 the lowest grain yield stability. Regarding grain yield by the years and the localities, the fertile inbred line L3 was the most stable and L11 the least stable. A cluster analysis carried out based on the obtained dendagrams showed some differences in the origin, i.e. genetic distance among the same inbred lines with different cytoplasmic source. The fertile lines and the C-sterile lines (L1, L2 and L3) therefore showed certain similarity in clustering and divergence, unlike the S-sterile lines. The fertile line L12 has a unique origin and represents a single cluster, and when it comes to divergence, it attains a big genetic distance from the other lines, regardless of their cytoplasmic source. In addition to scientific and research importance, the obtained results have also practical importance in hybrid maize seed production.- Cilj istraživanja bio je da se odredi stabilnost prinosa i komponenata prinosa dvanaest inbred linija kukuruza različitog tipa citoplazme (cms-C, cms-S i fertilna). Pošlo se od pretpostavke da su proučavane inbred linije kukuruza različite genetičke osnove i da različito reaguju na faktore spoljašnje sredine što utiče na različitu stabilnost prinosa i komponenti prinosa zrna. Ogled sa inbred linijama postavljen je na dve lokacije tokom 2008 i 2009 godine. Četvorofaktorijalna analiza varijanse (genotip-citoplazma-godina-lokacija) urađena je za ispitivane osobine: visina biljke do klipa, visina biljke do vrha metlice, prinos, dužina klipa, broj redova zrna, broj zrna u redu, dubina zrna, masa 1000 semena, srednje okrugla frakcija, srednje krupno pljosnata frakcija i srednje krupno okrugla frakcija semena. Korelacione veze između proučavanih osobina inbred linija analizirane su primenom jednačine višestruke korelacije i regresije. Izvršena je procena stabilnosti prinosa (Hühn, 1990) i urađena je klaster analiza za svaki izvor citoplazme (Rohlf, 2000). Na osnovu analize varijanse utvrđeno je da postoje veoma značajne razlike između inbred linija za sve ispitivane osobine kao i interakcije inbred linija sa ispitivanim faktorima (tip citoplazme, godina i lokacija). Najveći prinos zrna ostvarila je inbred linija L1 (3,3 t ha-1), a najmanji L10 (1,5 t ha-1). Tip citoplazme veoma značajno utiče na proučavane osobine inbred linija. Variranje prinosa zrna inbred linija u zavisnosti od tipa citoplazme (cms-C, cms-S i fertilna) bilo je veoma značajno kod inbred linija L2, L5, L7, L10 i L12. Prosečan prinos zrna inbred linija je veoma značajno (P≤1%) varirao u zavisnosti od ispitivanih godina i lokacija. Veći prinos (2,9 t ha-1) ostvaren 2008. godine u odnosu na 2009. godinu (2,12 t ha-1). Na prvoj lokaciji ostvaren je veći prinos (2,72 t ha-1) u odnosu na drugu lokaciju (2,31 t ha-1). Korelacioni odnosi kod inbred linija sa različitim tipom citoplazme razlikovali su se kod sledećih osobina: visina biljke do klipa sa dubinom zrna, masom 1000 semena i srednje krupno pljosnatom frakcijom; visina biljke do vrha metlice sa dužinom klipa, srednje krupno pljosnatom frakcijom i srednje krupno okruglom frakcijom; dužina klipa sa brojem redova zrna, brojem zrna u redu i srednje krupno okruglom frakcijom; broj redova zrna sa srednje krupno pljosnatom frakcijom i prinosom; broj zrna u redu sa dubinom zrna; dubina zrna sa srednje okruglom frakcijom semena; masa 1000 semena sa srednje okruglom i srednje krupno okruglom frakcijom semena. Kod preostalih ispitivanih osobina odnosno njihovih međusobnih korelacionih odnosa nisu utvrđene razlike između sva tri izvora citoplazme. Inbred linija L10 sa C izvorom citoplazme pokazala je najveću, a L12 najmanju stabilnost prinosa zrna po ispitivanim godinama i lokacijama. Kod inbred linija sa S tipom sterilnosti L12 je bila najstabilnija, a najnestabilnija za prinos zrna bila je L10. Fertilna inbred linija L3 bila je najstabilnija, a L11 najnestabilnija za osobinu prinos zrna po ispitivanim godinama i lokacijama. Urađena klaster analiza na osnovu dobijenih dendrograma pokazala je razlike u pripadnosti odnosno genetičkoj distanci između istih inbred linija ali sa različitim izvorom citoplazme. Tako su fertilne linije i linije sa C izvorom sterilnosti (L1, L2 i L3) pokazale izvesnu sličnost u pogledu grupisanja i divergentnosti za razliku od linija sa S izvorom sterilnosti. Fertilna linija L12 po svojoj pripadnosti je jedinstvena i zastupa samo jedan klaster, a u pogledu divergentnosti nalazi se na velikoj genetičkoj distanci u odnosu na ostale linije bez obzira na izvor citoplazme. Dobijeni rezultati pored naučno-istraživačkog značaja imaju i praktični značaj u proizvodnji hibridnog semena kukuruza.- All metadata published by Europeana are available free of restriction under the Creative Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana