-
Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosusPšeničnik, Alen ...Most biosynthetic gene clusters (BGC) encoding the synthesis of important microbial secondary metabolites, such as antibiotics, are either silent or poorly expressed; therefore, to ensure a strong ... pipeline of novel antibiotics, there is a need to develop rapid and efficient strain development approaches. This study uses comparative genome analysis to instruct rational strain improvement, using Streptomyces rimosus, the producer of the important antibiotic oxytetracycline (OTC) as a model system. Sequencing of the genomes of two industrial strains M4018 and R6-500, developed independently from a common ancestor, identified large DNA rearrangements located at the chromosome end. We evaluated the effect of these genome deletions on the parental S. rimosus Type Strain (ATCC 10970) genome where introduction of a 145 kb deletion close to the OTC BGC in the Type Strain resulted in massive OTC overproduction, achieving titers that were equivalent to M4018 and R6-500. Transcriptome data supported the hypothesis that the reason for such an increase in OTC biosynthesis was due to enhanced transcription of the OTC BGC and not due to enhanced substrate supply. We also observed changes in the expression of other cryptic BGCs; some metabolites, undetectable in ATCC 10970, were now produced at high titers. This study demonstrated for the first time that the main force behind BGC overexpression is genome rearrangement. This new approach demonstrates great potential to activate cryptic gene clusters of yet unexplored natural products of medical and industrial value.Vir: mSystems [Elektronski vir]. - ISSN 2379-5077 (Vol. 9, iss.5, May 2024, str. 1-30)Vrsta gradiva - e-članek ; neleposlovje za odrasleLeto - 2024Jezik - angleškiCOBISS.SI-ID - 191381251
Avtor
Pšeničnik, Alen |
Slemc, Lucija |
Avbelj, Martina |
Tome, Miha |
Šala, Martin |
Herron, Paul R. |
Shmatkov, Maksym |
Petek, Marko, biotehnolog, 1983- |
Baebler, Špela |
Mrak, Peter, 1978 |
Hranueli, Daslav |
Starcevic, Antonio |
Hunter, Iain S. |
Petković, Hrvoje
Teme
aktinomicete |
Streptomyces rimosus |
tetraciklini |
biosinteza |
genome reduction |
antibiotic biosynthesis |
oxytetracycline |
cryptic metabolites
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Avtor | Pšeničnik, Alen ... |
Naslov | Oxytetracycline hyper-production through targeted genome reduction of Streptomyces rimosus |
Datum objave | 2024-01-05 |
COBISS.SI-ID | 191381251 |
Verzija objave v repozitoriju | Založnikova različica |
Licenca objave v repozitoriju | Creative Commons Priznanje avtorstva 4.0 Mednarodna |
Embargo | Takojšnja javna objava |
Projekti, iz katerih je bila financirana objava
Naziv | Akronim | Številka projekta | Financer |
---|---|---|---|
Mikrobiologija in biotehnologija živil in okolja | P4-0116-2022 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Biotehnologija in sistemska biologija rastlin | P4-0165-2022 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
|
Analitika in kemijska karakterizacija materialov ter procesov | P1-0034-2020 |
Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije |
Datoteke, ki spadajo k objavi
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Pšeničnik, Alen | 53621 |
Slemc, Lucija | 50527 |
Avbelj, Martina | 28299 |
Tome, Miha | 36377 |
Šala, Martin | 25442 |
Herron, Paul R. | ![]() |
Shmatkov, Maksym | ![]() |
Petek, Marko, biotehnolog, 1983- | 29617 |
Baebler, Špela | 19116 |
Mrak, Peter, 1978 | 33193 |
Hranueli, Daslav | ![]() |
Starcevic, Antonio | ![]() |
Hunter, Iain S. | ![]() |
Petković, Hrvoje | 13542 |
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Obvestilo
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Mesto prevzema | Status gradiva | Rezervacija |
---|
Prosimo, počakajte trenutek.