-
Comparison of the visual scoring method and semi-automatic image analysis for evaluating staining intensity of human cartilage sectionsAlibegović, Armin, 1962- ...Accurate estimation of postmortem interval (PMI) is crucial in forensic medicine. The hyaline cartilage, being predominantly composed of a dense extracellular matrix and partly resistant to factors ... influencing protein degradation, can be utilized for analyzing PMI intervals. Various staining methods are available for cartilage staining for PMI evaluation; however, the conventional visual scoring method for assessing staining intensity is susceptible to evaluator bias. This study compared the visual scoring method with a modified Bern score with semi-automatic image analysis. The cartilage samples were obtained from human cadavers with known time of death. Forty-five histological slices were prepared and stained using Alcian blue, Safranin-O with Fast green, Safranin-O without Fast green, Masson trichrome, and Sirius red. Ten evaluators visually scored each sample on a scale of 0 to 3. A semi-automatic analysis was conducted on the same images using the deconvolution plugin of the ImageJ software by three independent evaluators. Linear regression was used to assess the correlation between the mean grey value and the mean Bern score from all evaluators. The results showed strong correlations across all evaluated staining techniques (r ≥ 0.77, p < 0.0001), with Masson trichrome staining exhibiting the highest correlation. The intra-class correlation coefficients between the independent semi-automatic assessments were excellent for all five stainings (ICC ≥ 0.965). Accordingly, semi-automatic image analysis can be a suitable replacement for the visual scoring method, particularly when no procedural artifacts are present.Vir: Image analysis & stereology : official journal of the International Society for Stereology. - ISSN 1580-3139 (Vol. 43, no. 2, 2024, str. 131-137)Vrsta gradiva - članek, sestavni del ; neleposlovje za odrasleLeto - 2024Jezik - angleškiCOBISS.SI-ID - 195734019
Avtor
Alibegović, Armin, 1962- |
Umek, Nejc |
Pušnik, Luka, 1996- |
Zubiavrre Martinez, Inigo
Teme
Bern score |
cartilage |
histology |
postmortem interval |
semi-automatic analysis |
visual scoring method |
rezultat Bern |
hrustanec |
histologija |
postmortalni interval |
polavtomatska analiza |
metoda vizualnega točkovanja
![loading ... loading ...](themes/default/img/ajax-loading.gif)
vir: Image analysis & stereology : official journal of the International Society for Stereology. - ISSN 1580-3139 (Vol. 43, no. 2, 2024, str. 131-137)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
![loading ... loading ...](themes/default/img/ajax-loading.gif)
Vnos na polico
Trajna povezava
- URL:
Faktor vpliva
Dostop do baze podatkov JCR je dovoljen samo uporabnikom iz Slovenije. Vaš trenutni IP-naslov ni na seznamu dovoljenih za dostop, zato je potrebna avtentikacija z ustreznim računom AAI.
Leto | Faktor vpliva | Izdaja | Kategorija | Razvrstitev | ||||
---|---|---|---|---|---|---|---|---|
JCR | SNIP | JCR | SNIP | JCR | SNIP | JCR | SNIP |
Baze podatkov, v katerih je revija indeksirana
Ime baze podatkov | Področje | Leto |
---|
Povezave do osebnih bibliografij avtorjev | Povezave do podatkov o raziskovalcih v sistemu SICRIS |
---|---|
Alibegović, Armin, 1962- | 28259 |
Umek, Nejc | 51820 |
Pušnik, Luka, 1996- | 57868 |
Zubiavrre Martinez, Inigo | ![]() |
Izberite prevzemno mesto:
Prevzem gradiva po pošti
Obvestilo
Gesla v Splošnem geslovniku COBISS
Izbira mesta prevzema
Mesto prevzema | Status gradiva | Rezervacija |
---|
Prosimo, počakajte trenutek.