UP - logo
Narodna in univerzitetna knjižnica, Ljubljana (NUK)
Naročanje gradiva za izposojo na dom
Naročanje gradiva za izposojo v čitalnice
Naročanje kopij člankov
Urnik dostave gradiva z oznako DS v signaturi
  • Superoxide release and cytopathogenic effects in gingival fibroblasts exposed to human immunodeficiency virus (HIV) and herpes simplex virus (HSV) = Vpliv virusa imunske pomanjkljivosti (HIV) in herpes simpleks virusa (HSV) na sproščanje superoksida in citopatogene učinke pri fibroblastih dlesne
    Skalerič, Uroš, 1945-2023 ; Venkateshan, C... ; Wahl, Sharon M.
    Background. Clinical signs of periodontal disase and herpes simplex virus (HSV) infections are recognized complications in HIV infected patients. The aim of this study was to evaluate whether viral ... infections such as HIV and HSVinduce the release of superoxide and contribute to cytopathogenic effects and cell death of gingival fibroblasts. Materials and methods. Primary cultures of gingival fibroblasts were prepared by the method of explanti culture. Fibroblasts were exposed to HIV-1 and HSV-1 and the relase of superoxide measured spectrophotometrically by the reduction of ferricytochromeC for the period of 5 hours. Cytopathogenic effects and cell death of fibroblasts exposed to HIV-1 and HSV-1 were observed by light microscopy and the release of 14V-labeled adenine during the 4 days observation period. Results. HIV-1 induced up to 0.5 nmol and HSV-1 up to 1.2 nmol of superoxide from gingival fibroblasts in the 5 hours observation period. The release of O2-was only partially suppressed by the addition of superoxide dismutase. HIV-1 induced only minor cytopathogenic effects and dealth of gingival fibroblasts in the 4 days observation period. HSV-1, however, induced pronounced cytopathogenic effects and complete lysis of gingival fibroblasts after 4 days of the observation period. The cytolysis of fibroblasts exposed to HSV-1 was not prevented by the addition of superoxide dismutase. Conclusions. Guman gingival fibroblasts after an exposure to HIV-1 released only a low amount of superoxide and showed only minor morphological changes and cell deaths. HSV-1 induced a relatively high level of superoxide release in gingival fibroblasts, which might contribute to pronounced cytopathogenic effects and cytolysis of these cells during the 4 days observation period. We are concluding that superoxide released from HSV exposed gingival fibroblasts could play a role in pathogenesis of oral herpes simplex virus infections.
    Vir: Zdravniški vestnik : glasilo Slovenskega zdravniškega društva = Slovenian medical journal = journal of Slovenian Medical Association. - ISSN 1318-0347 (Letn. 67, supl. 2, jun. 1998, str. II-87-II-90)
    Vrsta gradiva - članek, sestavni del
    Leto - 1998
    Jezik - angleški
    COBISS.SI-ID - 7664345

Vnos na polico