Baig, M. M. Q., Hafiz, I. A., Abbasi, N. A., Yaseen, M., Akram, Z. and Donnelly, D. J. 2012. Reduced-statureRosaspecies through in vitro mutagenesis. Can. J. Plant Sci. 92: 1049â1055. Plant height ...is one of the main attributes affecting general appeal and beauty of roses (Rosa spp.). Among the highly scented rose species, R. gruss an teplitz, R. centifolia, and R. borboniana, have great potential horticultural and commercial value. However, their large plant size detracts from recent trends towards selection of smaller plants for emerging markets and high-density plantations. This study aimed to produce reduced-stature plants through in vitro mutagenesis using gamma irradiation (Coââ). Shoot tips cut from micropropagated shoots were exposed up to 120 Gy. Irradiated shoot tips were micropropagated for one culture cycle. Surviving shoots were rooted in vitro then acclimatized for 2 mo in a greenhouse. The shoot tip LDâ â after gamma irradiation was species-dependent and 33â54 Gy. In this dose range, survival during in vitro rooting and acclimatization was also affected; this was 64 to 24% and 34 to 14% of control values, respectively. Acclimatized transplants were 17 to 56% smaller with 16 to 51% less leaf area compared with the controls. In order to ascertain stability putative reduced-stature roses will be monitored for vegetative and floral characteristics over the next few years. This study adds to the ongoing efforts to obtain reduced-stature rose plants for horticultural purposes.
Fennel (Foeniculum vulgare Mill.) is an important, well-known aromatic and medicinal herb. Fifty accessions of fennel were collected from different parts of Pakistan and evaluated for important ...characteristics like seed germination percentage (ger %), days to initiation of flowering, plant height, stem girth, nodal distance, umbel diameter, days to 50% maturity, days to harvesting, seed yield per row, weight of 100 seeds, Harvest index (%). Genomic DNA of the accessions was extracted and subjected to RAPD analysis in order to ascertain their genetic diversity. Twenty-four out of 30 decimer primers generated 145 clear bands and 70 (48%) were polymorphic. Sixteen primers OPA-01, OPA-03, OPA-04, OPA-05, OPA-07, OPA-10, OPA-11, OPA-14, OPA-15, OPA-18, AC-11, AC-14, AC-15, AC-16, AC-18 and AC-20 gave polymorphism for different characters. About 66.6% of polymorphic primers generated the highest index to resolve genetic diversity even in small number of accessions. Seven accessions from Punjab, 3 from NWFP, one from Balochistan and one from Northern Areas of Pakistan had appeared with promising characters.
Effect of priming with potassium nitrate and dehusking on seed germination of Gladiolus (Gladiolus alatus) Ramzan, A. (Pir Mehr Ali Shah, Arid Agriculture Univ., Rawalpindi (Pakistan). Dept. of Horticulture); Hafiz, I.A. (Pir Mehr Ali Shah, Arid Agriculture Univ., Rawalpindi (Pakistan). Dept. of Horticulture); Ahmad, T. (Pir Mehr Ali Shah, Arid Agriculture Univ., Rawalpindi (Pakistan). Dept. of Horticulture) ...
Pakistan journal of botany,
01/2010, Letnik:
42, Številka:
1
Journal Article
Recenzirano
Gladiolus (Gladiolus alatus), belonging to the family Iridaceae is rated as the most popular flower in the world at commercial scale. The effect of different concentrations of KNO3 (1, 2, 3,4,5 and 0 ...%) on seed germination percentage, time required for 50% germination and on mean germination time (MGT) was studied under controlled conditions. Best germination rate of 92% was achieved in T6 (distilled water) followed by 80% in T1 (1% KNO3) and 70% in T2 (2% KNO3). Minimum time required for 50% germination i.e., 8 days was obtained with T6 (distilled water) and in the same way shortest mean germination time required by seeds was 15 days in T6. Bulb gained maximum weight (0.6467 g) and diameter (9.49 mm) in T3 (3% KNO3). Likewise, this treatment also resulted in an acquisition of 14 cm seedling length and a positive correlation was found between seedling length and growth parameters of bulb i.e., weight and diameter. In another experiment, effect of dehusking on seed germination was tested. Seed without husk gave the promising outcome of 74% germination while seeds with husk merely acquired 63% germination after 30 days.
Department of Cardiac Surgery, Imam Reza Hospital, Mashhad University of Medical Sciences, Iran
*Corresponding author. Tel.: +98 511 8405559; fax: +98 511 7277749. E-mail address : ...hadi61{at}gmail.com (H. Javan).
Aneurysm of a saphenous vein graft (SVG) is a rare but fatal complication of coronary artery bypass graft (CABG) surgery. The development of SVG aneurysms appears usually about 10–20 years after the operation at an estimated rate of <1%. A 68-year-old male was referred to the emergency department after frequent episodes of dyspnea, chest pain and hemoptysis. He previously had CABG surgery one year before. The physical examination was normal. Chest radiogram showed a left pulmonary midzone mass. CT-angiogram demonstrated a large aortic pseudoaneurysm (6.36 x 6.06 cm) in the middle part of the ascending aorta. After sternotomy, the ascending aorta above sinotubular junction near the origin of brachiocephalic artery was resected and replaced with a tube graft. The patient was transferred to ICU with stable hemodynamic status. SVG aneurysm should be considered while encountering mediastinal mass or undiagnosed cardiopulmonary symptoms in patients with a previous history of CABG because of its rarity and overlap of symptoms with other thoracic, pulmonary, and cardiac diseases. Surgery seems to be the treatment of choice to reduce the risk of rupture and embolism.
Key Words: Coronary artery bypass graft; Saphenous vein; Aneurysm
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This study demonstrates a protocol for in vitro regeneration of Gerbera jamesonii cv. 'Sunglow' developed by culturing leaf segments on Murashige and Skoog (MS) medium supplemented with 1.0, 1.5, ...2.0, 2.5 and 3.0 mg L super(-1) indole-3-butyric acid (IBA), naphthalene acetic acid (NAA), 2,4-dichlorophenoxy acid (2,4-D) and 1.0, 2.0, 3.0, 4.0 and 5.0 mg L super(-1) benzyl adenine (BAP) and kinetin (Kin). The treatment, 1.5 mg l super(-1) 2,4-D gave 100% callus induction with a friable, nodular and creamish white callus. BAP gave fair callus growth with compact and brownish callus. However, Kin failed to produce callus. Shoot regeneration was assayed with 1.0, 2.0, 3.0, 4.0 and 5.0 mg L super(-1) BAP and Kin. BAP at 3.0 mg l super(-1) showed 76.67% shoot regeneration, 4 shoots per explant and shoot length of 9 cm, whereas same concentration of Kin gave only 16.67% regenerated shoots, 1.97 shoots per explant and length of 5.167 cm. In vitro root induction was determined by using 0.5, 1.0, 1.5 and 2.0 mg L super(-1) indole-3-acetic acid (IAA) and NAA. IAA at 1.5 mg L super(-1) exhibited a rooting percentage of 97.67%, whereas similar concentration of NAA gave relatively less rooting percentage of 60.67%. IAA gave thick roots with maximum root number (7.567/per explant) and length (7.33 cm), on the contrary, NAA gave relatively thin roots having less number of 3.567 roots with a length of 4.667 cm.
Rose is a beautiful flower having commercial and ornamental value. In order to establish protocol shoot tips explants of Rosa gruss an teplitz and Rosa centifolia were proliferated in vitro using MS ...medium supplemented with different levels of benzylaminopurine (0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 mg l super(-1)). Maximum numbers of shoots (3.906), shoot length (3.106 cm), fresh weight (178.47 mg) and dry weight (43.06 mg) was recorded at 1.0 mg l super(-1) BAP. For induction of root, uniform micro-shoots were excised and transferred to the rooting medium (1/2 MS macro, micro elements and vitamins) supplemented with 20 g l super(-1)sucrose and different concentrations (0.00, 0.25, 0.50, 1.0, 1.5 and 2.0 mg l super(-1)) of indole-3-butyric acid (IBA). IBA increased culture rooting percentage (89.375), number of roots (8.7188) and root length (3.5781 cm) more efficiently at 0.50 mg l super(-1).
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