Abstract
Baltim Eastern and Northern gas fields in the offshore Nile Delta have very high gas condensate accumulations. Therefore, the present research evaluates Abu Madi and Qawasim Formations and ...defines the petrophysical parameters for them using various data from five wells composed of wireline logs (gamma-ray, density, neutron, sonic, resistivity), core data, pressure data, and cross-plots. In the current study, the formations of the main reservoirs were evaluated qualitatively and quantitatively based on the petrophysical analysis to assess the production potential. Based on the lithological identification, the two main reservoirs (Abu Madi and Qawasim Formations) are composed of sandstone, calcareous shale, and siltstone. The main petrophysical parameters (Shale volume, effective porosity, net thickness, and fluid saturations) were mapped to track the areal petrophysical variations in the field. The results of the petrophysical analysis reveal that the main reservoirs are promising for the hydrocarbon potential with effective porosity of 18%, low shale content with an average value of about 21%, higher gas saturation of average value of nearly 58%, net reservoir thickness ranges from 25.5 to 131.5 m, net pay thickness (effective thickness) ranges from 6 to 61 m. Also, the conventional core analysis affirms that the main reservoirs are of good effective porosity with high horizontal and vertical permeability values. There is a good match between the well-log results and the pressure data with the production data (DST “perforation tests”). Baltim East (BE3) well has the most desired petrophysical characteristics in the Baltim East gas field, while, the Baltim North-1 (BN1) well showed the most favorable petrophysical parameters in the Baltim North gas field. Different fluid contacts (gas water contact GWC) were detected by integrating all reservoir pressures. The integration of different data in our present work (well logs, core measurements, and pressure data) could reduce the drilling risks and help to determine the best locations for future exploration and development, which is considered a big challenge in the petroleum industry.
Abstract
Background
Egypt has agreed and ratified international regulations that strict child labor. However, the country still struggles with high prevalence of child labor and the associated ...negative social and health effects. The objective of this study was to identify the prevalence and determinants of work-related injuries among working children in Egypt.
Methods
This study involved a secondary data analysis of the National Child Labor Survey (NCLS) conducted in 2010 by The Central Agency for Public Mobilization and Statistics (CAPMAS) in Egypt with technical and financial support from the ILO’s International Program on the Elimination of Child Labor (IPEC) through its Statistical Information and Monitoring Program on Child Labor (SIMPOC). The total number of working children who responded to questions of work-related injuries in the NCLS child questionnaire was 7485 children.
Results
The prevalence of work-related injuries among working children in Egypt was estimated as 24.1% (95% CI: 22.0%—26.2%), of whom the majority were superficial wounds (87.3%). Among children who reported work-related injuries, 57.9% did not stop work or schooling because of the most serious injury, while 39.6% had stopped temporarily and 2.6% had stopped completely. The main determinants of work-related injuries among working children in the study sample were gender (boys), age of starting work (5–11 years), type of main economic activity (industry and services), type of main workplace (plantation, farms, or garden), the average work hours per week (28 h or more), and exposure to ergonomic and safety, and chemical hazards at work.
Conclusions
The estimated high prevalence of work-related injuries among working children aged 5–17 years in Egypt raises the health risks concerns associated with child labor. Findings of this study on the determinants of work-related injuries could guide policies and interventions to combat child labor and the associated health risks, including work-related injuries.
Breast cancer (BC) is the commonest type of female cancer worldwide and a leading cause of cancer-related deaths. Perlecan expression increases in aggressive breast cancers, pointing to a possible ...significance of this novel target therapy. Consequently, the current research investigates the immunohistochemical expression of Perlecan by tumor cells in female breast cancer and the correlation of such expression with the pathologic parameters of the tumors.
A retrospective cross-sectional investigation was carried out. Seventy-four formalin-fixed, paraffin-embedded breast carcinoma tissue samples from patients undergoing modified radical mastectomy (MRM) or conservative breast surgery (CBS) were collected from the pathology department at Kasr El Aini Hospital. The paraffin blocks were sectioned and immunostained with Perlecan, and their expression was investigated in tumor cells and evaluated according to the H-score and classified into low and high expression. The expression was statistically correlated with the clinicopathological parameters of the cases.
Perlecan expression was low in 41 cases (55.4%) and high in 33 cases (44.6%). It showed a statistically insignificant correlation with all studied parameters, but increased Perlecan expression was directly associated with poor tumor prognostic factors including higher tumor grade, advanced T stage, N3 stage, advanced anatomic stage, high Ki-67 index, positive lymphovascular invasion and perineural invasion, luminal B molecular subtype, and HER2 over-expression.
Perlecan expression measured by immunohistochemical staining is associated with aggressive characteristics in breast cancer, suggesting that Perlecan may help in cancer progression and can be investigated as a possible target therapy.
Microelements are well recognized as an essential approach in the field of aquaculture nutrition. Thus, this study aimed to evaluate copper (Cu) inclusion (0, 0.5, 1, and 2 mg/kg) on Striped catfish ...performances. Fish fed the Cu-incorporated diets for 60 days, then their growth behavior, antioxidative capacity, and intestinal and liver histological features were evaluated. The results showed a marked enhancement in Striped catfish's growth behavior fed 1–2 mg/kg of Cu, as shown by the final weight, weight gain, and specific growth rate. The feed and protein efficiency ratios were significantly affected by Cu in a dose-dependent manner. The highest level of Cu was accumulated in the whole body, muscle, liver, and gills of fish fed 2 mg/kg of Cu. The carcass composition of Striped catfish showed higher protein content in groups received 0.5, 1, and 2 mg/kg Cu in a linear and quadratic manner (
p
=0.001). The ash content was quadratically increased in Striped catfish fed 2 mg/kg Cu (
p
=0.001). However, no marked effects were observed on the moisture and lipid contents and the somatic indices (
p
>0.05). The incorporation of Cu showed meaningfully increased superoxide dismutase, catalase, and glutathione peroxidase but decreased malondialdehyde level in Striped catfish. The villous height exhibited visible growth and branching with increased doses of Cu without a significant increase in the goblet cells. No abnormal features were observed in the liver and hepatocytes of fish treated with Cu. It can be concluded that Cu is required at 1–2 mg/kg for better performances of Striped catfish.
A total of one-hundred and fourteen
Capsicum annum
L. samples were collected from Qassim and Al-Baha regions, Saudi Arabia in the growing season of 2021–2022, showing virus like symptoms including, ...mottling, mosaic, chlorosis, leaf distortion, stunted growth, and necrotic lesions, dwarfing of leaves and fruits. These samples were tested against important viruses infecting pepper using ELISA kits. According to ELISA results, 80/114 (70%) samples were found to be infected with one or more of the tested viruses. However, incidence of the PMMoV was observed in 18/21 (85.7%) and 23/59 (38.98%) from Al-Baha and Qassim regions respectively. Based on highly positive ELISA samples of PMMoV three samples were selected for biological detection using mechanical inoculation with sap prepared and the results showed that systemic mosaic, mottling, necrotic spots, yellowing on
N. tabacum, N. benthamiana, N. glutinosa, Solanum nigrum and
necrotic and chlorotic spots on
Chenopodium quinoa
and
C. amranticolor
, while local lesions symptoms were produced on
Datura stramonium.
According to the ELISA results, positive samples from Qassim and Al-Baha regions were amplified by RT-PCR analysis, and 474-bp fragment was amplified using PMMoV specific primers. Two directional sequencing was done for the amplified products and the sequences of the virus isolates were submitted in the NCBI database with the following accession numbers, OP723368, OP723369, OP723370, OP723371, OP723372. The phylogenetic analysis showed the close relationship our isolates with other isolation of PMMoV and pairwise nucleotide sequence identity analysis revealed that these PMMoV isolates shared their identity between 92.5 and 100% with PMMoV isolates available in the databases. This study provides comprehensive report regarding the diagnosis of chili infecting viruses through serological and characterization of PMMoV through serological, biological, and molecular methods in the kingdom of Saudi Arabia.
The exacerbation of climatic changes helped to increase the risk of plant diseases in the world. The novelty of this study lies in the manufacture of therapeutic nutrients using nanotechnology with ...strong effectiveness against plant disease. Based on this concept, we mycosynthesized bimetallic ZnO-CuO nanoparticles (NPs), alternatives to reduce the spread of Vicia faba Fusarium wilt disease, which is one of the world’s most imperative cultivated crops. The article’s uniqueness comes in the utilization of ZnO-CuO nanoparticles to carry out two crucial tasks: therapeutic nutrients and managing Fusarium disease. To evaluate the resistance of infected plants, disease index (DI), photosynthetic pigments, osmolytes, oxidative stress and yield parameters were assessed. NPs of ZnO, CuO, and ZnO-CuO were mycosynthesized using a biomass filtrate of Aspergillus fumigatus OQ519856. DI reached 87.5%, due to Fusarium infection, and, as a result, a severe decrease in growth characters, photosynthetic pigments, total soluble carbohydrates, and proteins as well as yield parameters was observed. Infected plants produced more of the studied metabolites and antioxidants. On the other hand, the treatment with CuO-ZnO NPs led to a great decline in the DI by 22.5% and increased the protection by 74.28%. A clear improvement in growth characters, photosynthetic pigments and a high content of carbohydrates and proteins was also observed in both healthy and infected plants as a result of CuO-ZnO NPs treatment. Remarkably, CuO-ZnO NPs significantly increased the yield parameters, i.e., pods/plant and pod weight, by 146.1% and 228.8%, respectively. It could be suggested that foliar application of NPs of ZnO, CuO, and ZnO-CuO could be commercially used as antifusarial agents and strong elicitors of induced systemic resistance.
Background
Bacteriocins are proteinaceous compounds produced from lactic acid bacteria. Bacteriocins are well-known for their antibacterial potential and safety for application in food. However, the ...commercial availability of bacteriocin is facing several limitations; among them is the low yield and short stability period. That calls for a new strategy for overcoming these hurdles. Among these approaches is incorporating bacteriocin in nanoparticles. So, the aim of this study was to enhance the plantaricin produced from isolated
Lactobacillus plantarum
strain using nanotechnology.
Results
In this study, the
pln
EF genes encoding plantaricin EF have been identified and sequenced (accession number of MN172264.1). The extracted bacteriocin (EX-PL) was obtained by the ammonium sulfate method. Then, it was used for biosynthesizing plantaricin-incorporated silver nanoparticles (PL-SNPs). The synthesized nanoparticles were confirmed by SEM-EDAX analysis. The antibacterial activity of both combined (PL-SNPs) and extracted plantaricin (EX-PL) were tested against some strains of foodborne pathogenic bacteria. The results revealed that the antibacterial activities were increased by 99.2% on the combination of bacteriocin with the silver nanoparticle. The MIC of EX-PL (7.6 mg/mL) has been lowered after incorporating into silver nanoparticles and reached 0.004 mg/mL for PL-SNPs. Despite that extracted plantaricin showed no inhibitory activity towards
Listeria monocytogenes
, plantaricin-incorporated silver nanoparticles displayed inhibitory activity against this strain. Furthermore, the stability period at 4 °C was increased from 5 days to 60 days for EX-PL and PL-SNPs, respectively.
Conclusions
Plantaricin-incorporated silver nanoparticles possess higher antibacterial activity and more stability than the free one, which makes it more fitting for combating foodborne pathogens and open more fields for applications in both food and pharmaceutical industries.
Graphical abstract
Colchicine acts upstream in the cytokines cascade by inhibiting the nod-like receptor protein 3 (NLRP3) inflammasome while interleukin 6 (IL-6) receptor antagonists, such as tocilizumab, block the ...end result of the cytokines cascade. Hence, adding colchicine to tocilizumab with the aim of blocking the early and end products of the cytokines cascade, might reduce the risk of developing cytokine storm.
We aim to conduct an open-label randomized controlled trial to evaluate the efficacy and safety of adding colchicine to tocilizumab among patients with severe COVID-19 pneumonia to reduce the rate of invasive mechanical ventilation and mortality. We will include patients with severe COVID-19 pneumonia who received tocilizumab according to our local guidelines. Enrolled patients will be then randomized in 1:1 to colchicine versus no colchicine. Patients will be followed up for 30 days. The primary outcome is the rate of invasive mechanical ventilation and will be determined using Cox proportional hazard model.
Given colchicine's ease of use, low cost, good safety profile, and having different anti-inflammatory mechanism of action than other IL-6 blockade, colchicine might serve as a potential anti-inflammatory agent among patients with severe COVID-19 pneumonia. This study will provide valuable insights on the use of colchicine in severe COVID-19 when added to IL-6 antagonists.
The Medical Research Center and Institutional Review Board at Hamad Medical Corporation in Qatar approved the study protocol (MRC-01-21-299). Results of the analysis will be submitted for publication in a peer-reviewed journal.
PDF
