Oxidative stress and inflammation are the predominant cause of chronic diseases, including multiple forms of cancers. Prevention of oxidative stress and inflammation is considered to be a target for ...preventing these disorders due to their significant roles in various degenerative diseases. Various natural products and plant extracts prevent the process of free radical- induced damages.
The present study evaluated the biological properties of Thottea siliquosa, belonging to the family Aristolochiaceae, which is a traditionally used Ayurvedic plant.
Antioxidant assays carried out were DPPH, FRAP, hydrogen peroxide scavenging, and hemolysis inhibition assay; nitric oxide and lipoxygenase inhibition assays were used for anti-inflammatory studies. Anticancer activity was evaluated using human endometrial and breast cancer cells by MTT assay. Bioactive compounds present in T. siliquosa were identified by LCMS and each was docked with various cancer targets, including EGFR, VEGFR, GST, COX2, and Lipooxygenase.
The results of the present study showed antioxidant properties of the methanolic crude extract of T. siliquosa as DPPH radical scavenging (110.40 ± 4.5 μg/mL), FRAP capacity (41.1 ± 6.2), and peroxide scavenging (233.4 ± 14.2 μg/mL). Besides, anti-inflammatory properties were also evident in terms of nitric oxide radical scavenging (28.76± 3.9 μg/mL) and lipoxygenase inhibition (39.2 ± 3.2 μg/mL) assays. In silico analysis confirmed the inhibitory potential of the bioactive compounds of T. siliquosa against cancer drug targets such as EGFR, VEGFR, and inflammatory enzymes cyclooxygenase as well as lipooxygenase. Further, the anticancer activity of the extract has been identified against human endometrial and breast cancer cells. The possible mechanism of anticancer action of the extract is mediated through the apoptosis induction mechanism acting through increased caspase and APAF-1 expressions.
The study thus concludes that T. siliquosa showed significant antioxidant, anti-inflammatory and anticancer properties. Further studies together with a bioassay-guided fractionation may identify possible bioactive compounds.
Our goal is to evaluate the current dietary offerings available for residents, survey how this food selection impacts their overall wellness, and measure any effect gained from broadening the current ...nutritional choices available.
An anonymous, paper survey about GME nutrition selection was distributed to residents during a dedicated wellness hour. The surveys were collected and analyzed as follows. The z-test for proportions was performed to determine the proportion of residents who answered yes or no to the resident lounge snack survey questionnaires. The first question was on snack satisfaction was a response on a scale of 1–10 and is presented as means with confidence interval. A chi-squared test was performed to see if there is a correlation between resident snack satisfaction and yes or no responses to survey questions on dietary restriction, limited choices, nutritional needs, sugar crash, patient advice, home food quality and nutritional for wellness. The last two questions were written responses and were tabulated and enumerated for responses falling into respective food categories.
All residents’ (100%) surveyed responded, “yes” to the nutritional wellness. While the “Yes” responses to home food quality, diet restriction, limited choices, and sugar crash were 8.3%, 20.8%, 25% and 58.3%, respectively. All residents surveyed (100%) responded “No” to the nutritional needs and patient advice. The mean snack satisfaction score was 4, 95% CI 3.32–4.68. Only home food quality was found to be significantly related to resident satisfaction (P < 0.05). The most popular response to the suggestion list for GME snacks were fruit (n = 6) and jerky (n = 5). The most popular response to the Do Not Need List for GME Snack Area were no response (n = 9), cookies (n = 6) and chips (n = 6).
Residents prefer healthy snacks such as fruit and do not want unhealthy snacks like cookies and chips. There is an association between resident’s snack satisfaction on the current snack choices and the home food quality.
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Aim： FMS-like receptor tyrosine kinase （FLT3） is expressed in some normal hematopoietic cell types and plays an important role in the pathogenesis of acute myeloid leukemia （AML）. In this study, we ...examined the effects of triazoloacridino＇ne C-1305, an antitumor compound, on AML cells with different FLT3 status in vitro. Methods： A panel of human, leukemic cell lines with different FLT3 status was used, including FLT3 internal tandem duplication mutations （FLT3-1TD, MV-4-11）, wild-type FLT3 （RS-4-11） and nulI-FLT3 （U937） cells. Cell proliferation was estimated using MTT assays, and apoptosis was studied with flow cytometry and fluorescence microscopy. FLT3 kinase activity （phosphorylation of FLT3 at Tyr591） was determined with ELISA and Western blotting. FLT3 downstream signaling proteins involving AKT, MAPK and STAT5 were examined by Western blotting. RNA silencing was used to decrease the endogenous FLT3. Results： The mutant FLT3-1TD cells were more sensitive to C-1305 than the wild-type FLT3 and nulI-FLT3 cells （the ICso values measured at 24 h were 1.2±0.17, 2.0±09, 7.6±1.6 μmol/L, respectively）. C-1305 （1-10 μmol/L） dose-dependently inhibited the kinase activity of FLT3, which was more pronounced in the mutant FLT3-1TD cells than in the wild-type FLT3 cells. Furthermore, C-1305 dose-dependently decreased the phosphorylation of STAT5 and MAPK and the inhibitory phosphorylation of Bad, and induced time- and dose-dependent apoptosis in the 3 cell lines with the nulI-FLT3 cells being the least susceptible to C-1305-induced apoptosis. Knockdown of FLT3 with siRNA significantly decreased C-1305-induced cytotoxicity in the mutant FLT3-ITD cells. Conclusion： C-1305 induces apoptosis in FLT3-ITD-expressing human leukemia cells in vitro, suggesting that mutated FLT3 kinase can be a new target for C-1305, and C-1305 may be a drug candidate for the therapeutic intervention in FLT3-associated AML.
As part of the German Jewish Cultural Heritage project, the contributors to this volume examine the cultural influence of the German-speaking Jewish intelligentsia around the world. This influence ...continues to be felt in many countries of origin and exile without being adequately reflected in collective consciousness. This volume makes a substantive contribution to the search for traces of German-speaking Jewish culture in the wake of emigration.
Provider: - Institution: - Data provided by Europeana Collections- Hamburg, Universität Hamburg, Diss., 2013- All metadata published by Europeana are available free of restriction under the Creative ...Commons CC0 1.0 Universal Public Domain Dedication. However, Europeana requests that you actively acknowledge and give attribution to all metadata sources including Europeana
Protein structures can provide invaluable information, both for reasoning about biological processes and for enabling interventions such as structure-based drug development or targeted mutagenesis. ...After decades of effort, 17% of the total residues in human protein sequences are covered by an experimentally determined structure
. Here we markedly expand the structural coverage of the proteome by applying the state-of-the-art machine learning method, AlphaFold
, at a scale that covers almost the entire human proteome (98.5% of human proteins). The resulting dataset covers 58% of residues with a confident prediction, of which a subset (36% of all residues) have very high confidence. We introduce several metrics developed by building on the AlphaFold model and use them to interpret the dataset, identifying strong multi-domain predictions as well as regions that are likely to be disordered. Finally, we provide some case studies to illustrate how high-quality predictions could be used to generate biological hypotheses. We are making our predictions freely available to the community and anticipate that routine large-scale and high-accuracy structure prediction will become an important tool that will allow new questions to be addressed from a structural perspective.
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