DNA molecules originating from animals and plants can be retrieved directly from sediments and have been used for reconstructing both contemporary and past ecosystems. However, the extent to which ...such ‘dirt’ DNA reflects taxonomic richness and structural diversity remains contentious. Here, we couple second generation high‐throughput sequencing with 16S mitochondrial DNA (mtDNA) meta‐barcoding, to explore the accuracy and sensitivity of ‘dirt’ DNA as an indicator of vertebrate diversity, from soil sampled at safari parks, zoological gardens and farms with known species compositions. PCR amplification was successful in the full pH range of the investigated soils (6.2 ± 0.2 to 8.3 ± 0.2), but inhibition was detected in extracts from soil of high organic content. DNA movement (leaching) through strata was evident in some sporadic cases and is influenced by soil texture and structure. We find that DNA from the soil surface reflects overall taxonomic richness and relative biomass of individual species. However, one species that was recently introduced was not detected. Furthermore, animal behaviour was shown to influence DNA deposition rates. The approach potentially provides a quick methodological alternative to classical ecological surveys of biodiversity, and most reliable results are obtained with spatial sample replicates, while relative amounts of soil processed per site is of less importance.
Plateau dunes are unique landforms which formed as erosion relicts in blow-out areas in Late Pleistocene sandy deposits. They are negative forms in contrast to normal dunes where sand is accumulated ...(positive form). Plateau dunes are located in the European Aeolian Sand Belt in the Netherlands, Germany and Denmark and they are flat-topped features with a bog in the central part often covered by a thin aeolian sand layer. A conceptual model of formation and further development of plateau dunes in time are presented in detail, using 14C dates of buried peat layers and OSL dates of sandy sediments. Special focus is on the influence of climate factors, pedology, and human activity on the formation of plateau dunes.
•Conceptual model of plateau dune formation•Pedology as a landforming factor•OSL dating of aeolian deposits•Climate impact on blanket peat formation•Human destruction of pre-existing vegetation cover
