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•Old LFL treatment with B. adusta was investigated through batch and continuous tests.•B. adusta produced MnP with cellulose, glucose, and malt as co-substrates.•Irregular ...co-substrate dosages significantly increased COD and sCOD removal.•COD removals of 63% and 51% were achieved with glucose and cellulose, respectively.•sCOD removals of 53% and 51% were achieved with glucose and cellulose, respectively.
Conventional wastewater treatment technologies are ineffective for remediation of old LandFill Leachate (LFL), and innovative approaches to achieve satisfactory removal of this recalcitrant fraction are needed. This study focused on old LFL treatment with a selected fungal strain, Bjerkandera adusta MUT 2295, through batch and continuous tests, using packed-bed bioreactors under non-sterile conditions. To optimize the process performance, diverse types of co-substrates were used, including milled cellulose from beverage cups waste material. Extracellular enzyme production was assayed, in batch tests, as a function of a) cellulose concentration, b) leachate initial Chemical Oxygen Demand (COD) and Soluble COD (sCOD), and c) co-substrate type. Bioreactors were dosed with an initial start-up of glucose (Rg) or cellulose (Rc). An additional glucose dosage was provided in both reactors, leading to significant performance increases. The highest COD and sCOD removals were i) 63% and 53% in Rg and ii) 54% and 51% in Rc.
In environmental biotechnology applications for wastewater treatment, bacterial-based bioprocesses are mostly implemented; on the contrary, the application of fungal-based bioprocesses, is still ...challenging under non-sterile conditions. In a previous laboratory-scale study, we showed that when specific tannins are used as the sole carbon source, fungi can play a key role in the microbial community, under non-sterile conditions and in the long term. In a previous study, an engineered ecosystem, based on fungal tannin biodegradation, was successfully tested in a laboratory-scale bioreactor under non-sterile conditions. In the present study, a kinetic and stoichiometric characterisation of the biomass developed therein was performed through the application of respirometric techniques applied to the biomass collected from the above-mentioned reactor. To this aim, a respirometric set-up was specifically adapted to obtain valuable information from tannin-degrading fungal biofilms. A mathematical model was also developed and applied to describe both the respirometric profiles and the experimental data collected from the laboratory-scale tests performed in the bioreactor. The microbial growth was described through a Monod-type kinetic equation as a first approach. Substrate inhibition, decay rate and tannin hydrolysis process were included to better describe the behaviour of immobilised biomass selected in the tannin-degrading bioreactor. The model was implemented in AQUASIM using the specific tool Biofilm Compartment to simulate the attached fungal biofilm. Biofilm features and transport parameters were either measured or assumed from the literature. Key kinetic and stoichiometric unknown parameters were successfully estimated, overcoming critical steps for scaling-up a novel fungal-based technology for tannins biodegradation.
A number of bacteria and fungi are known to degrade tannins. In this study, the efficiency of the white-rot fungus, Bjerkandera adusta MUT 2295, was evaluated for the treatment of a synthetic ...solution prepared with tannic acid. Tests were performed in continuously fed, bench-scale, packed-bed reactors, operated under non-sterile conditions with biomass immobilized within PolyUrethane Foam cubes (PUFs). The main parameters monitored to evaluate the process efficiency were: soluble Chemical Oxygen Demand (sCOD), Total Organic Carbon (TOC) removal, and activities. of Tannase and Lignin Peroxidase. At the end of the process, additional parameters were evaluated, including the increase of fungal dry weight and the presence of ergosterol. The reactor was operative for 210 days, with maximum sCOD and TOC removal of 81% and 73%, respectively. The reduction of sCOD and TOC were positively correlated with the detection of Tannase and Lignin Peroxidase (LiP) activities. Increases in biomass within the PUF cubes was associated with increases in ergosterol concentrations. This study proved that the fungal-based system tested was efficient for the degradation of tannic acid over a period of time, and under non-sterile conditions.
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•Tannic acid was treated in a continuously-fed reactor using a white-rot fungus.•The reactor was operated for 210 days under non-sterile conditions.•Depletion of the stable recalcitrant compound was achieved after 164 days.•The process was associated with the detection of oxidative enzymes.•Fungal treatment for remediation of tannic acid was demonstrated to be feasible.
•The growth of white-rot fungi was tested on polyethylene carriers containing cellulose.•Basidiomycetes could cover fully the carriers with the highest cellulose percentage.•B. adusta could grow up ...to 60% of carrier surface in not-sterile conditions.•B. adusta growth was associated with 25 ± 4% Quebracho tannin sCOD removal.•B. adusta and A. tubingensis could grow together and remove Quebracho sCOD up to 15±4%
In the last decades, White-rot Fungi bioremediation potential has been widely investigated, providing remarkable results toward several recalcitrant molecules. However, full-scale applications based on fungi are not employed yet and little is known about their optimal operating conditions, such as (i) their ability to grow without sterile conditions, (ii) co-substrate requirements and (iii) the accurate carrier design for fungal growth. In this study, several batch tests were performed as preliminary steps to evaluate the possible design of a pilot-scale reactor based on fungal biomass to be operated under not-sterile conditions in the removal of Quebracho natural tannin. The tests were performed to verify fungal affinity, including Basidiomycetes and Ascomycetes for innovative cellulose-containing carriers compared to commonly employed PolyUrethane Foam Cubes. In particular, four fungi, including three Basidiomycetes White-rot Fungi, Bjerkandera adusta, Phanerochaete chrysosporium and Tyromyces chioneus and the Ascomycota strain Aspergillus tubingensis, were employed. As a first step, fungi were tested to evaluate their ability to attach and grow onto 12 types of innovative carriers made by High-Density PolyEthylene and containing cellulose in different percentages. Other tests were performed without sterile conditions. In particular, fungal abilities (i) to attach and grow onto two different types of support, including cellulose-containing carrier and polyurethane foam cubes and (ii) to biotransform recalcitrant molecules (Quebracho natural tannin) (iii) to grow and operate synergistically in a consortium of two fungi, were evaluated. The main parameters evaluated were soluble Chemical Oxygen Demand (sCOD) reduction and dry weight increase. Basidiomycetes showed high affinity for cellulose-containing carrierswith the highest cellulose percentage (7%) achieving full colonization and 60% coverage, in sterile conditions and not- sterile conditions, respectively. These results were associated with a Quebracho sCOD removal of 25 ± 4%, without sterility. When combined, the two selected strains, Bjerkandera adusta and Aspergillus tubingensis were able to grow on carriers and to remove up to 15 ± 4% of tannins recalcitrant sCOD. This study provides evidence of (i) Basidiomycetes high affinity for cellulose-containing carriers that could favour fungi attachment in sterile and not-sterile conditions and (ii) the feasibility of a combined use of Ascomycetes and Basidiomycetes in bioremediation.
Periodontitis (PD) is a risk factor for acute myocardial infarction (AMI). C-reactive protein (CRP) is elevated in PD. The aim of this study was to investigate biochemical alterations among AMI with ...PD. Thirty non-AMI (25 non-PD and only 5 PD) and 30 AMI (13 PD and 17 non-PD) patients were participated. Serum CRP, tumor necrosis factor alpha (TNF-α), total cholesterol, low-density lipoprotein cholesterol, protein carbonyl (PC) contents, soluble vascular cell adhesion molecule-1 (sVCAM-1), adiponectin, creatine kinase, resistin, catalase and superoxide dismutase (SOD) levels were measured. AMI-PD elicited significant differences in percentage hypertension, diabetes, serum creatine kinase, cholesterol and low-density lipoprotein cholesterol. CRP among AMI-PD and non-AMI-PD was increased by 73.4% and 31.3%, respectively. The level of PC contents was increased significantly among AMI-PD and non-AMI-PD by 47.62% and 33.3%, respectively. Catalase and SOD levels were significantly decreased in AMI-PD by 33.7% and 34.1%, respectively; however, their levels among non-AMI-PD were significantly increased by 35.7% and 28%, respectively. TNF-α, sVCAM-1 and resistin levels among AMI-PD were increased by 134.3%, 68.8% and 25.5%, respectively; however, TNF-α and sVCAM-1 levels among non-AMI-PD were increased significantly by 21.4% and 29.4%, respectively. Adiponectin level produced insignificant changes. PD prevalence among AMI associated with elevated serum CRP, PC contents, sVCAM-1, TNF-α and resistin levels concurrent with declines in SOD and catalase enzymes. In conclusion, among nondiabetic, nonsmoking patients suffering from AMI, PD is highly prevalent and associated with elevated serum CRP, PC contents, sVCAM-1, TNF-α and resistin levels, associated with significant declines in antioxidant enzymes with insignificant change in serum adiponectin level.
Medium‐ to large‐scale marine protected areas (MPAs) are playing an increasingly important role in global marine biodiversity conservation. A key question is “how do we collect relevant data on ...biodiversity and population trends to inform their design and measure success?”
This question is particularly relevant for marine taxa that are difficult to survey, such as sharks and rays, and where populations may occur over vast areas. Environmental DNA (eDNA) metabarcoding has proven to be an effective sampling method and may provide a solution to these challenges; however, it remains unclear how its sampling effectiveness compares with traditionally used methods for elasmobranch surveying.
Here, we directly compared the efficacy of eDNA metabarcoding and demersal longline deployments to survey elasmobranchs across 31 sites over a 55,000 km2 area of the Kimberley and Roebuck Australian Marine Parks in Australia's North‐west Marine Parks Network.
In total, we documented 49 unique elasmobranch taxa, 36 of which were detected by eDNA and 32 (from a total of 815 captured sharks) by longline. A combined approach yielded over 34% more elasmobranch taxa than either method alone.
Site community compositions varied between the two survey methods; notably eDNA was able to detect species from outside of the immediate sampling area, although this was still consistent with a detection radius of a few kilometres, highlighting a particular use in rugose reef habitats where it is difficult to deploy longlines.
In investigating the quantitative use of eDNA data, we report that eDNA metabarcoding read abundance (count) data was poorly correlated with longline aggregate catch (count and biomass) data across raw, relative and rank abundance measures. However, we found that in multivariate analyses, both binary (presence–absence) and abundance (after square‐root transformation) datasets produced highly similar ordination patterns, largely segregating method (eDNA vs. longline), followed by latitude and depth.
Lastly, we identified required levels of eDNA replication and longline deployments to maximize captured elasmobranch diversity.
This study sets an integrated, georeferenced baseline and long‐term monitoring approach for the management and conservation of elasmobranch diversity within this unique marine park network.
The CDF Silicon Vertex Trigger Ashmanskas, Bill; Barchiesi, A.; Bardi, A. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
02/2004, Letnik:
518, Številka:
1
Journal Article
Recenzirano
Odprti dostop
The Collider Detector at Fermilab (CDF) experiment's Silicon Vertex Trigger (SVT) is a system of 150 custom 9U VME boards that reconstructs axial tracks in the CDF silicon strip detector in a
15
μs
...pipeline. SVT's
35
μm
impact parameter resolution enables CDF's Level 2 trigger to distinguish primary and secondary particles, and hence to collect large samples of hadronic bottom and charm decays. We review some of SVT's key design features. Speed is achieved with custom VLSI pattern recognition, linearized track fitting, pipelining, and parallel processing. Testing and reliability are aided by built-in logic state analysis and test-data sourcing at each board's input and output, a common interboard data link, and a universal “Merger” board for data fan-in/fan-out. Speed and adaptability are enhanced by use of modern FPGAs.
In Moving Bed Biofilm Reactors (MBBR) the biofilm grows protected within small plastic carriers (known as biofilm carriers), which are designed with high internal surface area. The biological ...wastewater treatment process consists of adding biofilm carriers in aerated or anoxic tanks to support biofilm attachment and growth. Some of the authors conceived, designed and realised an improved carrier (new shape and material) for fungal biofilm development. The improved biofilm carriers were tested in laboratory conditions and good results were obtained. This new biofilm carrier will be used to treat cellulosic (tannery and papermill) wastewaters and is grafted with cellulose fibers for the self-sustainability of the fungal biomass. This will facilitate substrate adhesion, due to biochemical capabilities of the selected strains, which involve secretion of extracellular enzymes, that can break down substrate by combined action of several degradative processes, such as demethylation, oxidative cleavage of the propane side chain, cleavage of ether bonds between monomers etc.
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