The sequestration of organic carbon in seafloor sediments plays a key role in regulating global climate; however, human activities can disturb previously-sequestered carbon stocks, potentially ...reducing the capacity of the ocean to store CO
. Recent studies revealed profound seafloor impacts and sedimentary carbon loss due to fishing and shipping, yet most other human activities in the ocean have been overlooked. Here, we present an assessment of organic carbon disturbance related to the globally-extensive subsea telecommunications cable network. Up to 2.82-11.26 Mt of organic carbon worldwide has been disturbed as a result of cable burial, in water depths of up to 2000 m. While orders of magnitude lower than that disturbed by bottom fishing, it is a non-trivial amount that is absent from global budgets. Future offshore developments that disturb the seafloor should consider the safeguarding of carbon stocks, across the full spectrum of Blue Economy industries.
We report on an analysis of the gas and dust budget in the interstellar medium (ISM) of the Large Magellanic Cloud (LMC). Recent observations from the Spitzer Space Telescope enable us to study the ...mid-infrared dust excess of asymptotic giant branch (AGB) stars in the LMC. This is the first time we can quantitatively assess the gas and dust input from AGB stars over a complete galaxy, fully based on observations. The integrated mass-loss rate over all intermediate and high mass-loss rate carbon-rich AGB candidates in the LMC is 8.5 × 10−3 M⊙ yr−1, up to 2.1 × 10−2 M⊙ yr−1. This number could be increased up to 2.7 × 10−2 M⊙ yr−1 if oxygen-rich stars are included. This is overall consistent with theoretical expectations, considering the star formation rate (SFR) when these low- and intermediate-mass stars where formed, and the initial mass functions. AGB stars are one of the most important gas sources in the LMC, with supernovae (SNe), which produces about 2–4 × 10−2 M⊙ yr−1. At the moment, the SFR exceeds the gas feedback from AGB stars and SNe in the LMC, and the current star formation depends on gas already present in the ISM. This suggests that as the gas in the ISM is exhausted, the SFR will eventually decline in the LMC, unless gas is supplied externally. Our estimates suggest ‘a missing dust-mass problem’ in the LMC, which is similarly found in high-z galaxies: the accumulated dust mass from AGB stars and possibly SNe over the dust lifetime (400–800 Myr) is significant less than the dust mass in the ISM. Another dust source is required, possibly related to star-forming regions.
Whether supernovae are major sources of dust in galaxies is a long-standing debate. We present infrared and submillimeter photometry and spectroscopy from the Herschel Space Observatory of the Crab ...Nebula between 51 and 670 mu m as part of the Mass Loss from Evolved StarS program. We compare the emission detected with Herschel with multiwavelength data including millimeter, radio, mid-infrared, and archive optical images. We carefully remove the synchrotron component using the Herschel and Planck fluxes measured in the same epoch. The contribution from line emission is removed using Herschel spectroscopy combined with Infrared Space Observatory archive data. Several forbidden lines of carbon, oxygen, and nitrogen are detected where multiple velocity components are resolved, deduced to be from the nitrogen-depleted, carbon-rich ejecta. No spectral lines are detected in the SPIRE wavebands; in the PACS bands, the line contribution is 5% and 10% at 70 and 100 mu m and negligible at 160 mu m. After subtracting the synchrotron and line emission, the remaining far-infrared continuum can be fit with two dust components. Assuming standard interstellar silicates, the mass of the cooler component is 0.24 super(+0.32) sub(-0.08) M sub(middot in circle) for T = 28.1 super(+5.5) sub(-3.2) K. Amorphous carbon grains require 0.11 + or - 0.01 M sub(middot in circle) of dust with T = 33.8 super(+2.3) sub(-1.8) K. A single temperature modified blackbody with 0.14 M sub(middot in circle) and 0.08 M sub(middot in circle) for silicate and carbon dust, respectively, provides an adequate fit to the far-infrared region of the spectral energy distribution but is a poor fit at 24-500 mu m. The Crab Nebula has condensed most of the relevant refractory elements into dust, suggesting the formation of dust in core-collapse supernova ejecta is efficient.
We present new Herschel photometric and spectroscopic observations of Supernova 1987A, carried out in 2012. Our dedicated photometric measurements provide new 70 mu m data and improved imaging ...quality at 100 and 160 mu m compared to previous observations in 2010. Our Herschel spectra show only weak CO line emission, and provide an upper limit for the 63 mu m OI line flux, eliminating the possibility that line contaminations distort the previously estimated dust mass. The far-infrared spectral energy distribution (SED) is well fitted by thermal emission from cold dust. The newly measured 70 mu m flux constrains the dust temperature, limiting it to nearly a single temperature. The far-infrared emission can be fitted by 0.5 + or - 0.1 M sub(middot in circle) of amorphous carbon, about a factor of two larger than the current nucleosynthetic mass prediction for carbon. The observation of SiO molecules at early and late phases suggests that silicates may also have formed and we could fit the SED with a combination of 0.3 M sub(middot in circle) of amorphous carbon and 0.5 M sub(middot in circle) of silicates, totalling 0.8 M sub(middot in circle) of dust. Our analysis thus supports the presence of a large dust reservoir in the ejecta of SN 1987A. The inferred dust mass suggests that supernovae can be an important source of dust in the interstellar medium, from local to high-redshift galaxies.
The luminous Type IIn SN 2010jl shows strong signs of interaction between the SN ejecta and dense circumstellar material. Dust may be present in the unshocked ejecta; the cool, dense shell (CDS) ...between the shocks in the interaction region; or in the circumstellar medium (CSM). We present and model new optical and infrared photometry and spectroscopy of SN 2010jl from 82 to 1367 days since explosion. We evaluate the photometric and spectroscopic evolution using the radiative transfer codes mocassin and damocles, respectively. We propose an interaction scenario and investigate the resulting dust formation scenarios and dust masses. We find that SN 2010jl has been continuously forming dust based on the evolution of its infrared emission and optical spectra. There is evidence for preexisting dust in the CSM as well as new dust formation in the CDS and/or ejecta. We estimate that 0.005-0.01 M of predominantly carbon dust grains has formed in SN 2010jl by ∼1400 days post-outburst.
Aims/hypothesis
Rapamycin (sirolimus) is one of the primary immunosuppressants for islet transplantation. Yet there is evidence that the long-term treatment of islet-transplant patients with ...rapamycin may be responsible for subsequent loss of islet graft function and viability. Therefore, the primary objective of this study was to elucidate the molecular mechanism of rapamycin toxicity in beta cells.
Methods
Experiments were performed on isolated rat and human islets of Langerhans and MIN6 cells. The effects of rapamycin and the roles of mammalian target of rapamycin complex 2 (mTORC2)/protein kinase B (PKB) on beta cell signalling, function and viability were investigated using cell viability assays, insulin ELISA assays, kinase assays, western blotting, pharmacological inhibitors, small interfering (si)RNA and through the overproduction of a constitutively active mutant of PKB.
Results
Rapamycin treatment of MIN6 cells and islets of Langerhans resulted in a loss of cell function and viability. Although rapamycin acutely inhibited mTOR complex 1 (mTORC1), the toxic effects of rapamycin were more closely correlated to the dissociation and inactivation of mTORC2 and the inhibition of PKB. Indeed, the overproduction of constitutively active PKB protected islets from rapamycin toxicity whereas the inhibition of PKB led to a loss of cell viability. Moreover, the selective inactivation of mTORC2 using siRNA directed towards rapamycin-insensitive companion of target of rapamycin (RICTOR), mimicked the toxic effects of chronic rapamycin treatment.
Conclusions/interpretation
This report provides evidence that rapamycin toxicity is mediated by the inactivation of mTORC2 and the inhibition of PKB and thus reveals the molecular basis of rapamycin toxicity and the essential role of mTORC2 in maintaining beta cell function and survival.
Unhealthy diets are increasing contributors to poor health and mortality in low- and middle-income countries (LMICs). Government interventions targeting the structural drivers of unhealthy diets are ...needed to prevent these illnesses, including nutrition labelling regulations that create healthier food environments. Yet, implementation remains slow and uneven. One explanation for slow implementation highlights the role of politics, including powerful ideological discourse and its strategic deployment by economically powerful actors. In this article, we advance research on the politics of nutrition policies by analysing political discourse on nutrition labelling regulations within an influential and under-studied global institution: the World Trade Organization (WTO). We identified WTO Technical Barriers to Trade (TBT) Committee meeting minutes with reference to nutrition labelling policies proposed by Thailand, Chile, Indonesia, Peru, Ecuador, Bolivia, and Uruguay (2007–2019; n = 47). We analysed the frames, narratives, and normative claims that feature in inter-country discourse within TBT meetings and examined how actors mobilize ideological and material sources of power via these statements. We find that informal government challenges to nutrition labelling proposals within the Committee featured a narrative that individualized the causes of and solutions to poor diet, downplayed harms from industrialised food products, and framed state regulation as harmful and unjust. These non-technical claims mobilised neoliberal ideology and rhetoric to contest the normative legitimacy of members’ proposals and to de-socialize and de-politicize poor diets. Furthermore, high-income countries (HICs) re-framed policy goals to focus on individual determinants of poor nutrition whilst calling for their preferred policies to be adopted. Patterns of discourse within TBT meetings also had striking similarities with arguments raised by multi-national food corporations elsewhere. Our findings suggest that non-technical and ideological arguments raised during TBT meetings serve as inconspicuous tools through which nutrition labelling policies in LMICs are undermined by HICs, industry, and the powerful ideology of neoliberalism.
•Critical discourse analysis of informal challenges to nutrition labelling polices.•Informal challenges occurred at the World Trade Organization (WTO), 2007–2019.•Challenges individualized, de-socialized, and de-politicized unhealthy diets.•Challenges invoked neoliberal ideology and industry rhetoric.•Discourse, and its deployment by powerful actors, may inconspicuously shape policy.
Children and young people are increasingly likely to receive information regarding inherited health risks relevant to their genetic relatives and themselves. We reviewed the literature to determine ...what children and young people (21 years and younger) understand about inherited conditions and their attitudes towards genetic testing. We screened 1815 s to identify 20 studies representing the perspectives of 1811 children and young people between the ages of 6 and 21 years (1498 children or young people at general population‐level risk from 9 studies, 313 affected/at risk from 15 studies). Children and young people at general population‐level risk demonstrated a basic understanding that disease predisposition can be inherited within families. Those affected by or at risk of genetic conditions inferred their genetic status from observable, relational characteristics within their family and the results of personal genetic testing if it had occurred, but some misunderstandings of important genetic concepts were evident. Children and young people expressed interest in and a willingness to undertake personal genetic testing, but also articulated concerns about the limitations and risks of testing. Paediatric patients require developmentally‐sensitive genetic counselling and support in navigating the unique landscape of their condition.
A significant number of pancreases procured for transplantation are deemed unsuitable due to concerns about graft quality and the associated risk of complications. However, this decision is ...subjective and some declined grafts may be suitable for transplantation. Ex vivo normothermic perfusion (EVNP) prior to transplantation may allow a more objective assessment of graft quality and reduce discard rates. We report ex vivo normothermic perfusion of human pancreases procured but declined for transplantation, with ABO‐compatible warm oxygenated packed red blood cells for 1–2 h. Five declined human pancreases were assessed using this technique after a median cold ischemia time of 13 h 19 min. One pancreas, with cold ischemia over 30 h, did not appear viable and was excluded. In the remaining pancreases, blood flow and pH were maintained throughout perfusion. Insulin secretion was observed in all four pancreases, but was lowest in an older donation after cardiac death pancreas. Amylase levels were highest in a gland with significant fat infiltration. This is the first study to assess the perfusion, injury, as measured by amylase, and exocrine function of human pancreases using EVNP and demonstrates the feasibility of the approach, although further refinements are required.
This study reports the first use of a short period of ex vivo normothermic perfusion to assess the quality of human donor pancreases deemed unsuitable for transplantation, reporting injury markers, endocrine function, and histopathology.
The origin of interstellar dust in galaxies is poorly understood, particularly the relative contributions from supernovae and the cool stellar winds of low-intermediate-mass stars. Recently, large ...masses of newly formed dust have been discovered in the ejecta of core-collapse supernovae. Here, we present Herschel Photodetector Array Camera and Spectrometer (PACS) and Spectral and Photometric Imaging Receiver (SPIRE) photometry at 70-500
m of the historical, young supernova remnants: Kepler and Tycho, both thought to be the remnants of Type Ia explosion events. We detect a warm dust component in Kepler's remnant with
and mass
; this is spatially coincident with thermal X-ray emission and optical knots and filaments, consistent with the warm dust originating in the circumstellar material swept up by the primary blast wave of the remnant. Similarly for Tycho's remnant, we detect warm dust at
with mass
. Comparing the spatial distribution of the warm dust with X-rays from the ejecta and swept-up medium, and Hα emission arising from the post-shock edge, we show that the warm dust is swept up interstellar material. We find no evidence of a cool (25-50 K) component of dust with mass ≥0.07 M⊙ as observed in core-collapse remnants of massive stars. Neither the warm or cold dust components detected here are spatially coincident with supernova ejecta material. We compare the lack of observed supernova dust with a theoretical model of dust formation in Type Ia remnants which predicts dust masses of 88(17) × 10−3 M⊙ for ejecta expanding into ambient surrounding densities of 1(5) cm−3. The model predicts that silicon- and carbon-rich dust grains will encounter, at most, the interior edge of the observed dust emission at ∼400 years, confirming that the majority of the warm dust originates from swept-up circumstellar or interstellar grains (for Kepler and Tycho, respectively). The lack of cold dust grains in the ejecta suggests that Type Ia remnants do not produce substantial quantities of iron-rich dust grains and has important consequences for the 'missing' iron mass observed in ejecta. Finally, although, we cannot completely rule out a small mass of freshly formed supernova dust, the Herschel observations confirm that significantly less dust forms in the ejecta of Type Ia supernovae than in the remnants of core-collapse explosions.
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