Inflammation has significant roles in all phases of tumor development, including initiation, progression and metastasis. Interleukin-10 (IL-10) is a well-known immuno-modulatory cytokine with an ...anti-inflammatory activity. Lack of IL-10 allows induction of pro-inflammatory cytokines and hinders anti-tumor immunity, thereby favoring tumor growth. The IL-10 network is among the most important paths linking cancer and inflammation. The simple node-and-edge network representation is useful, but limited, hampering the understanding of the mechanistic details of signaling pathways. Structural networks complete the missing parts, and provide details. The IL-10 structural network may shed light on the mechanisms through which disease-related mutations work and the pathogenesis of malignancies.
Using PRISM (a PRotein Interactions by Structural Matching tool), we constructed the structural network of IL-10, which includes its first and second degree protein neighbor interactions. We predicted the structures of complexes involved in these interactions, thereby enriching the available structural data. In order to reveal the significance of the interactions, we exploited mutations identified in cancer patients, mapping them onto key proteins of this network. We analyzed the effect of these mutations on the interactions, and demonstrated a relation between these and inflammation and cancer. Our results suggest that mutations that disrupt the interactions of IL-10 with its receptors (IL-10RA and IL-10RB) and α2-macroglobulin (A2M) may enhance inflammation and modulate anti-tumor immunity. Likewise, mutations that weaken the A2M-APP (amyloid precursor protein) association may increase the proliferative effect of APP through preventing β-amyloid degradation by the A2M receptor, and mutations that abolish the A2M-Kallikrein-13 (KLK13) interaction may lead to cell proliferation and metastasis through the destructive effect of KLK13 on the extracellular matrix.
Prediction of protein-protein interactions through structural matching can enrich the available cellular pathways. In addition, the structural data of protein complexes suggest how oncogenic mutations influence the interactions and explain their potential impact on IL-10 signaling in cancer and inflammation.
Objective: The aim of the current study is to investigate the strongest and weakest points of the three different structures of prosthodontic restorations constituting the coronal structure when ...considered as a whole: the remaining tooth, composite resin, and computer-aided design and computer-aided manufacturing (CAD/CAM) restorative materials. Methods: Seventy extracted caries-free molars, CAD/CAM blocks Lava Ultimate (LU), Vita Enamic (VE), IPS e.max CAD (IPS), and composite resin materials Clearfil Majesty Posterior CMP, Light Core LC, Filtek Bulk Fill Posterior FBP, EverX Posterior EP were used for this study. Dentin and CAD/CAM sections were embedded in acrylic. Clearfil SE Bond was used as adhesive material. Composite resin materials were applied to the dentin surface using a Teflon mold. LU and VE were sandblasted with 50-μm Al2O3 for 10-sec. IPS was etched with HF for 20-sec. Ceramic Primer-2 was applied to the surfaces. Composite bars (2.3x3 mm) were adhered to CAD/CAM blocks using RelyX-U200. In addition, CAD/CAM bars were also adhered to dentin. The shear bond strength test was performed. Failure modes were examined using a stereomicroscope. Differences were analysed using one-way ANOVA and Tukey Post Hoc test. Results: The highest shear bond strength values of the composite resin materials to dentin tissue were observed in EP (p<0.05). Shear bond strength values of composite resin materials to IPS were found higher than to LU and VE. The shear bond strengths of the three different CAD/CAM restorative materials to the dentin tissue were statistically similar. Conclusion: The type of composite resin materials affects the shear bond strength to dentin tissue and CAD/CAM restorative materials. However, the type of CAD/CAM restorative material does not affect the shear bond strength to dentin tissue.
The PRISM web server enables fast and accurate prediction of protein-protein interactions (PPIs). The prediction algorithm is knowledge-based. It combines structural similarity and accounts for ...evolutionary conservation in the template interfaces. The predicted models are stored in its repository. Given two protein structures, PRISM will provide a structural model of their complex if a matching template interface is available. Users can download the complex structure, retrieve the interface residues and visualize the complex model. The PRISM web server is user friendly, free and open to all users at http://cosbi.ku.edu.tr/prism.
To investigate the effects of preparation order and sandblasting procedures (different particle sizes and sandblasting times) on the wear, surface properties, and shear bond strength of zirconia ...material. A total of 130 test specimens were fabricated from Katana-Zirconia (n = 10). 70 test samples were sintered. Control, pre-sintered, and sintered groups were formed. The initial weights of the samples were measured. Sandblasting treatments were performed from a distance of 10-mm at 2-bar pressure for 10, 15, and 20-s with 29-µm and 50-µm Al
2
O
3
. The weight measurement was repeated. Subsequently, pre-sintered groups were sintered. Samples were scanned using a Nanovea-PS50. Ra, Rz, and Sa values were recorded. Then, samples were embedded in acrylic. Composite bars were fabricated using a Teflon mold and bonded to the samples with G-CEM Link-Force. The shear bond strength test was performed. The data were analyzed using the Shapiro-Wilk test and two-way analysis ANOVA (p < 0.05). The highest Ra values were 1.198 ± 0.105 (Group-6) for PSZ and 0.267 ± 0.015 (Group-6) for SZ. Sa data demonstrated the highest values of 4.628 ± 1.541 (Group-6) for PSZ. The highest SBS values were 15.35 ± 2.75 (Group-4) for PSZ and 12.76 ± 2.39 (Group-1) for SZ. PSZ showed higher wear amount, Ra, Rz, Sa, and SBS in Groups 4 and 5 (p < 0.05). Pre-sintered and sintered zirconia showed different dependencies on sandblasting parameters regarding weight loss, Ra, Rz, Sa, and SBS. Sandblasting zirconia materials with 50-µm Al
2
O
3
for 10 or 15-s before sintering may have beneficial effects on surface roughness and SBS.
Resin Matrix CAD/CAM Materials BAŞPINAR ALPER, Seval
Journal of International Dental Sciences,
04/2023, Letnik:
9, Številka:
1
Journal Article
CAD/CAM stands for computer-aided design and computer-aided manufacturing. CAD/CAM systems are used to speed up the design and production process in dentistry. With three-dimensional design and ...milling, various restorations can be fabricated without the need for a physical model. It is possible to scan the oral cavity and take digital impressions. The restoration can be designed with the software's help and fabricated in a shorter time without laboratory stages. In addition, the mechanical strength and edge compatibility of the restorations are improved and the fabrication of aesthetic restorations is aimed. CAD/CAM technology offers advantages such as time savings, elimination of potential errors, ease of use, and treatment quality. With the increasing demand for CAD/CAM restorations, various materials have been developed. Nowadays, CAD/CAM materials are widely available on the market and restorations can be produced from prefabricated blocks and discs. In this review, resin matrix CAD/CAM materials, which are relatively new on the market and claimed to have some positive properties for dental restorations, are described.
CAD/CAM, bilgisayar destekli tasarım ve bilgisayar destekli üretim anlamına gelmektedir. Diş hekimliğinde tasarım ve üretim sürecini hızlandırmak için CAD/CAM sistemleri kullanılmaktadır. Üç boyutlu tasarım ve frezeleme ile fiziksel bir modele ihtiyaç duymadan çeşitli restorasyonlar üretilebilmektedir. Ağız boşluğunu taramak ve dijital ölçü almak mümkündür. Restorasyon, yazılım vasıtasıyla tasarlanır ve laboratuvar aşaması olmadan daha kısa sürede üretilebilir. Ayrıca restorasyonların mekanik dayanımı ve kenar uyumluluğu iyileştirilir ve estetik restorasyonların üretilmesi amaçlanır. CAD/CAM teknolojisi zaman tasarrufu, olası hataların ortadan kaldırılması, kullanım kolaylığı ve tedavi kalitesi gibi avantajlar sunmaktadır. CAD/CAM restorasyonlarına yönelik artan talep ile birlikte çeşitli materyaller geliştirilmiştir. Günümüzde CAD/CAM malzemeleri piyasada yaygın olarak bulunmaktadır ve prefabrike bloklardan ve disklerden restorasyonlar üretilebilmektedir. Bu derlemede piyasada nispeten yeni olan ve dental restorasyonlar için bazı olumlu özelliklere sahip olduğu iddia edilen rezin matriks CAD/CAM materyaller anlatılmaktadır.
The objective of this study was to evaluate the safety and efficacy of transcatheter closure of ventricular septal defects (VSD) using the LifeTech™ multifunctional occluder device (MF-Konar).
...Clinical features and demographic characteristics and follow-up findings were evaluated retrospectively from three centers.
MF-Konar was used in 98 patients. The median age and weight of the patients were 3.8 years (range 5.4 months–50 years) and 15.3 kg (range 5.5–80 kg), respectively. The mean fluoroscopy time was 13.7 ± 8.2 min (range 3.4–42.6 min). Procedural success was obtained for 96 out of 98 patients (98%). In 54 out of 98 patients, closure was performed via the antegrade route. Major complications occurred in four patients (embolization in two, complete heart block in one, and device dislocation needing surgical treatment in one). All of the complications were treated successfully, and there was no mortality. Mild residual flow in eight patients (8%), new onset tricuspid valve insufficiency in one (moderate), and new onset aortic valve insufficiency in one (mild) were observed during a mean follow-up duration of 224 ± 149 (10–515) days. Minor rhythm disturbances were observed in eight patients.
Transcatheter closure of VSDs in selected patients using the LifeTech MF-Konar device seems effective. Its advantages are softer design, use of both an antegrade and retrograde approach, and an advanced smaller delivery system. Increasing the number of usage and the experience will provide more accurate data and low complication rates.
•MF-Konar is feasible, safe and efficacious For VSD closure for selected patients.•Success was 98% and major complications were 4% for VSD closure with MF-Konar.•MF-Konar can be used both antegrade and retrograde approach, smaller delivery system.•Do not close VSD if complete AV block occurred during the procedure.
Background
Current evidence about the low-acuity ED visits of old patients has been generated in various healthcare systems; however, this is not a well-researched area in middle-income countries by ...patients’ perspectives.
Methods
A study of 231 patients attending the ED of a tertiary public hospital was conducted using a structured questionnaire from June to August 2021. Eligible patients were community-dwelling, aged > 65 years, who presented with lower clinical urgency. The questionnaire tool was developed using questions from available studies on health service utilization. Additional commentary was obtained from the patients regarding on the reasons for attending the ED and was administered on weekdays between 8:00 am and 5:30 pm.
Results
The study included 221 patients over 65 years of age, with a mean age of 72.21 ± 4.2. A total of 58.4% of the patients thought that they should be examined urgently, 15.4% had symptoms that had lasted more than one month, and 59% requested emergency imaging. Patients preferred the ED via treatment, imaging, transportation opportunities of emergency department, past negative experiences, and access problems with family medicines or appointment problems with specialist outpatient clinics with expectations of continuity of care.
Conclusion
Old individuals have unique healthcare needs, and the motives behind this group of patients with low-acuity problems to apply to the ED is complex. As one of the key objectives of healthcare policies is to make sure that patients are assessed at proper time and place, studies that consider patient perspectives might lead to accurate conclusions for this aim.
To evaluate the usefulness of intestinal biomarkers in determining the presence of intestinal epithelial damage in neonatal calves with diarrhea caused by 4 etiologic agents.
40 neonatal calves that ...were healthy (n = 10) or had diarrhea (30).
The study was a cross-sectional study. Results of hematologic analyses and serum concentrations of intestinal fatty acid-binding protein (I-FABP), liver fatty acid-binding protein (L-FABP), trefoil factor 3 (TFF-3), Claudin-3 (CLDN-3), γ-enteric smooth muscle actin (ACTG2), intestinal alkaline phosphatase (IAP), interleukin-8 (IL-8), platelet-activating factor (PAF), and leptin (LP) were compared among calves grouped according to whether they were healthy (control group; G-1) or had diarrhea caused by K99
(G-2; n = 10), bovine rota- or coronavirus (G-3; 5 each), or
spp (G-4; 10).
Across the 3 time points at which blood samples were obtained and evaluated, the groups of calves with diarrhea generally had markedly higher mean serum concentrations of L-FABP, TFF-3, IAP, IL-8, and LP, compared with the control group. In addition, G-2 also consistently had markedly higher mean serum concentrations of I-FAB and ACTG2 and lower mean serum concentrations of CLDN-3, compared with the control group.
Results indicated that degree of intestinal epithelial damage differed among calves grouped by the etiologic agent of diarrhea and that such damage might have been more severe in calves with diarrhea caused by K99
. Additionally, our results indicated that serum concentrations of I-FABP, L-FABP, TFF-3, IAP, IL-8, ACTG2, LP, and CLDN-3 were useful biomarkers of intestinal epithelial damage in calves of the present study.
The purpose of the present study was to determine hypoxic brain damage in calves with perinatal asphyxia using brain-specific damage biomarkers. Ten healthy and 25 calves with perinatal asphyxia were ...enrolled in the study. Clinical examination, neurological status score, and laboratory analysis were performed at admission, 24, 48, and 72 h. Serum concentrations of ubiquitin carboxy-terminal hydrolysis 1 (UCHL1), calcium-binding protein B (S100B), adrenomodullin (ADM), activitin A (ACTA), neuron-specific enolase (NSE), glial fibrillary acidic protein (GFAP) and creatine kinase-brain (CK-B) were measured. Histopathological and immunohistochemical examinations of the brain tissue were performed in 13 nonsurvivor calves. The neurological status score of the calves with asphyxia was significantly (p < 0.05) lower. Mix metabolic-respiratory acidosis and hypoxemia were detected in calves with asphyxia. Serum UCHL1 and S100B were significantly (p < 0.05) increased, and NSE, ACTA, ADM, and CK-B were decreased (p < 0.05) in calves with asphyxia. Histopathological and immunohistochemical examinations confirmed the development of mild to severe hypoxic-ischemic encephalopathy. In conclusion, asphyxia and hypoxemia caused hypoxic-ischemic encephalopathy in perinatal calves. UCHL1 and S100B concentrations were found to be useful markers for the determination of hypoxic-ischemic encephalopathy in calves with perinatal asphyxia. Neurological status scores and some blood gas parameters were helpful in mortality prediction.
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