While spatial proteomics by fluorescence imaging has quickly become an essential discovery tool for researchers, fast and scalable methods to classify and embed single-cell protein distributions in ...such images are lacking. Here, we present the design and analysis of the results from the competition Human Protein Atlas - Single-Cell Classification hosted on the Kaggle platform. This represents a crowd-sourced competition to develop machine learning models trained on limited annotations to label single-cell protein patterns in fluorescent images. The particular challenges of this competition include class imbalance, weak labels and multi-label classification, prompting competitors to apply a wide range of approaches in their solutions. The winning models serve as the first subcellular omics tools that can annotate single-cell locations, extract single-cell features and capture cellular dynamics.
U posljednjih se desetak godina u medijima često govori o povećanom broju dijagnosticiranih slučajeva autizma u djetinjstvu. U ovom sustavnom pregledu razmatramo razvoj razumijevanja percepcije lica, ...jedne od specifičnijih značajki autizma, u svjetlu komorbiditeta s aleksitimijom i uznapredovalih statističkih metodologija istraživanja. Navodimo literaturu koja se bavi percepcijom lica u autizmu u različitim kognitivnim zadacima i upućuje na važnost često komorbidne aleksitimije, kao i literaturu koja koristi neuroslikovne metode istraživanja i potvrđuje važnost različitih uzoraka gledanja u autizmu pri analizi rezultata. U svrhu poboljšane obrazovne i socijalne integracije osoba s autizmom i/ili aleksitimijom predlažemo teme za daljnje istraživanje.
In the last few decades, the media have continuously reported on the increased number of diagnosis of Autism Spectrum Disorder (ASD). This systematic review looks at the evolution of research on face perception, one of the characteristics of ASD, in the light of recently reported prevalence of alexythimia comorbidity, as well as improved statistical methods in cognitive research. We review the literature on behavioural face perception testing in autism, showing the importance of a prevalent comorbidity of alexythimia, as well as neural evidence showing the importance of differential eye gaze patterns in autism for data analysis. We suggest outstanding questions in the field in order to improve the integration of people with ASD and alexithymia into the educational and social contexts.
Background: Carotid-femoral pulse wave velocity (cfPWV), acknowledged as a reliable proxy of arterial stiffness, is an independent predictor of cardiovascular (CV) events. Carotid-femoral PWV is ...considered the gold standard for the estimation of arterial stiffness. cfPWV is a demanding, time consuming and expensive method, and an estimated PWV (ePWV) has been suggested as an alternative method when cfPWV is not available. Our aim was to analyze the predictive role of ePWV for CV and all-cause mortality in the general population. Methods: In a stratified random sample of 1086 subjects from the general Croatian adult population (EH-UH study) (men 42.4%, average age 53 ± 16), subjects were followed for 17 years. ePWV was calculated using the following formula: ePWV = 9.587 − 0.402 × age + 4.560 × 10−3 × age2 − 2.621 × 10−5 × age2 × MBP + 3.176 × 10−3 × age × MBP − 1.832 × 10−2 × MBP. MBP= (DBP) + 0.4(SBP − DBP). Results: At the end of the follow-up period, there were 228 deaths (CV, stroke, cancer, dementia and degenerative diseases, COLD, and others 43.4%, 10.5%, 28.5%, 5.2%, 3.1%, 9.3%, respectively). In the third ePWV tercile, we observed more deaths due to CV disease than to cancer (20.5% vs. 51.04%). In a Cox regression analysis, for each increase in ePWV of 1 m/s, there was a 14% increase risk for CV death. In the subgroup of subjects with higher CV risk, we found ePWV to be a significant predictor of CV deaths (ePWV (m/s) CI 1.108; p < 0.029; HR 3.03, 95% CI 1.118–8.211). Conclusions: In subjects with high CV risk, ePWV was a significant and independent predictor of CV mortality.
In the last few decades, the media have continuously reported on the increased number of diagnosis of Autism Spectrum Disorder (ASD). This systematic review looks at the evolution of research on face ...perception, one of the characteristics of ASD, in the light of recently reported prevalence of alexythimia comorbidity, as well as improved statistical methods in cognitive research. We review the literature on behavioural face perception testing in autism, showing the importance of a prevalent comorbidity of alexythimia, as well as neural evidence showing the importance of differential eye gaze patterns in autism for data analysis. We suggest outstanding questions in the field in order to improve the integration of people with ASD and alexithymia into the educational and social contexts.
CAGAR syndrome Bošnjak, Vito; Miličević, Ana; Prkačin, Ingrid
Liječnički vjesnik,
04/2019, Letnik:
141, Številka:
suppl.1
Web Resource
Odprti dostop
We present the patient with the combination of different genetic and autoimmune diseases:Congenitally corrected transposition of great arteries(CCTGA),Gitelman syndrome,resistant arterial ...hypertension and urinary retention that was the first sign of multiple sclerosis. A 54-year old female patient presented to the department of internal medicine due to hypokalaemia and hypomagnesemia when Gitelman syndrome was diagnosed.Concentrations of potassium in blood measured from 2014 to 2019 were:2.9-3.1-3.2-3.3-3.7mmol/L.She also had resistant arterial hypertension which is uncommon in Gitelman sy.,but now is successfully controlled with antihypertensive therapy.The patient also suffers from urinary retention(lower than 3 L of urine)which is an atypical clinical presentation,but it was also the first sign of multiple sclerosis.Because of urinary retention she undergo miction cystography after which low residual volume(80mL)was detected and potential diverticula were suspected.She often develops fevers up to 42°C(due to sinusitis, otitis, UTI)during witch oedema of soft tissue is observed(she gains on her weight up to 15kg),that condition is considered and treated as hyperpyrexia syndrome.A positive history of hypersensitivity to many different drugs such as penicillin,ciprofloxacin,tramadol, metoclopramide,trospium chloride,ezetimib,ivabradine, clarithromycin,pregabalin was identifited.Statin and fibrates-induced myopathy and urinary retention while taking amitriptyline and carbamazepine were also observed. Gitelman syndrome has been considered as a benign variant of salt- losing nephropathies presenting asymptomatic or with mild symptoms.This view has since been challenged by recent reports emphasizing the phenotype variability and the potential severity of the disease.This is the first case where Gitelman sy. is associated with CCTGA,resistant arterial hypertension and urinary retention which all together can be described as a CAGAR syndrome.
We present a patient with ACEI induced angioedema after 5 years of therapy that caused not only swelling of mouth and upper airways but the whole body,which is an unusual clinical presentation. A ...73-year old male patient was admitted to the emergency care unit with breathing difficulties due to the upper airway swelling that lasted for 2 hours.He had hypertension,diabetes mellitus, hypothyroidism,chronic renal disease and multiple myeloma.He had ACEI in his therapy for the last 5 years.The patient also had had swelling of the tongue 5 and 2 years ago.He was given corticosteroids and antihistamines which successfully resolved the edema,so allergic angioedema was diagnosed. This time he did not react to corticosteroids and antihistamines.Intubation was not possible due to tongue edema.Acute asphyxia occurred after 8.5 hours and emergency tracheotomy was performed.The swelling of upper airways and body continued to develop without the stabilization of clinical state.Because of suspected hereditary angioedema,a subcutaneous injection of icatibant was given as a lifesaving procedure.It caused the regression of the edema. ACEI were excluded from the therapy.After a week, blood analysis showed normal C1-inhibitor and C4 levels. Bradykinin mediated angioedema,including hereditary and ACEI induced forms,does not respond to conventional antihistamine and corticosteroid therapy. Also,they are not associated with urticaria. ACEI inhibit bradykinin degradation because angiotensin II is a key factor for the inactivation of bradykinin. Hereditary angioedema was suspected due to these severe symptoms and unusual clinical presentation. Type III hereditary angioedema with the normal level of C1-inhibitor is very rare but is considered as a differential diagnosis in our case.
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