We have begun to examine the biological and toxic effects of ozone on Jurkat T cells incubated thereafter for 24, 48 and 72 h. Tissue culture medium was strengthened by adding 20% fetal calf serum ...with an albumin content of about 6 mg/ml. Ozonization was performed by exposing for 10 min a volume of cell suspension (4 x 105/ml) to an equal volume of a gas mixture composed of oxygen-ozone with precise ozone concentrations ranging from 1.5 up to 72 µg/ml (31.5-1512 µM). The proliferation index declined progressively and was ozone dose-dependent. The response of enzymatic activities varied depending upon the enzyme and ozone concentrations: glucose-6-phosphate dehydrogenase begins to increase at an ozone dose of 6 µg/ml (126 µM), reached a peak at 12 µg/ml (252 µM) and rapidly declined thereafter. On the other hand activities of superoxide dismutase, glutathione peroxidase and glutathione reductase increased progressively from the ozone concentration of 12 µg/ml. Thus, as we have observed in blood, the biological response is linked to the ozone dose that must reach a threshold to be effective.
A radio-guided surgery technique exploiting β− emitters is under development. It aims at a higher target-to-background activity ratio implying both a smaller radiopharmaceutical activity and the ...possibility of extending the technique to cases with a large uptake of surrounding healthy organs. Such technique requires a dedicated intraoperative probe detecting β- radiation. A first prototype has been developed relying on the low density and high light yield of the diphenylbutadiene doped para-therphenyl organic scintillator. The scintillation light produced in a cylindrical crystal, 5 mm in diameter and 3 mm in height, is guided to a photo-multiplier tube by optical fibres. The custom readout electronics is designed to optimize its usage in terms of feedback to the surgeon, portability and remote monitoring of the signal. Tests show that with a radiotracer activity comparable to those administered for diagnostic purposes the developed probe can detect a 0.1 ml cancerous residual of meningioma in a few seconds.
The disinfectant activity of ozone is well recognized and ozone is used worldwide for sterilization of water. The use of ozone as a complementary medical approach is less known, because it has mostly ...been used in an empirical fashion without a rational basis and appropriate controls. In spite of this drawback, the use of judicious and standardized ozone dosages can elicit the formation of ROS acting as natural physiological activators of several biological functions. There is now a reasonable understanding of a few mechanisms of action and, using classical pharmacological concepts, it appears possible to formulate a rationale for optimizing clinical applications. A further exciting development is that ozone, being an oxidizer, can upregulate the intracellular anti-oxidant enzymes eventually inhibiting the constant, life-long oxidative stress responsible for degenerative diseases and aging. Among various routes for the administration of ozone, the autohemotransfusion procedure, consisting in exposing blood to ozone, i.e. to a calculated and brief oxidative stress, appears safe, simple, inexpensive and amenable to be adjusted to different pathological states It is hoped that this review will help to dispel prejudices, to clarify that ozone toxicity can be tamed, to show that ozone can act as a bioregulator and to encourage controlled clinical investigations to evaluate definitely the validity of ozonetherapy.
Background: Empirical observations have shown that ozonated autohemotherapy markedly improves the symptoms of chronic limb ischemia (muscular pain at rest, intermittent claudication, etc ) in ...atherosclerotic patients, but me chanisms of action remain uncle ar. Aims : Human endothelial cells (HUVECs ) are known to release nitrogen monoxide (NO) and we investigated the biologic al effects of human ozonated serum on HUVECs in culture. Methods: We assessed the relevance of peroxidation, the release of NO as nitrite and of three classical cytokines. Results: The treatment of HUVECs with ozonated serum yields a dose dependent increase of thiobarbituric acid reactive substances (TBARS) and of hydrogen peroxide (H_2O_2) and a decrease of protein thiol groups (PTG). Concomitantly, in comparison to either the control or the oxygenated sample, there is a significant and steady increase of nitric oxide (NO) production; this is markedly enhanced by the addition of L-arginine (20 μM) and inhibited in the presence of the NO inhibitor, L-NAME (20 mM). The main media to r of ozone action is H_2O_2 as it has been shown either after its dire ct measurement or by the addition of 20, 40 and 100 μM. Moreover, during 24 hours incubation we have investigated the production of endothelin 1 (ET-1), E-selectin and Interleukin 8 (IL-8) and it appears that ozonation enhances IL-8, in hibits E-selectin and hardly modifies ET-1 production. Conclusions: It appears that reinfusion of ozonated blood, by enhancing re lease of NO, may induce vasodilation in ischemic areas and reduce hypoxia.
THERE is some anecdotal evidence that oxygen-ozone therapy may be beneficial in some human diseases. However so far only a few biochemical and pharmacodynamic mechanisms have been elucidated. On the ...basis of preliminary data we postulated that controlled ozone administration would promote an oxidative preconditioning preventing the hepatocellular damage mediated by free radicals. Six groups of rats were classified as follows: (1) negative control, using intraperitoneal sunflower oil; (2) positive control using carbon tetrachloride (CCl_4) as an oxidative challenge; (3) oxygen-ozone, pretreatment via rectal insufflation (15 sessions ) and after it, CCl_4; (4) oxygen, as group 3 but using oxygen only; (5) control oxygen-ozone, as group 3, but without CCl_4 ; group (6) control oxygen, as group 5, but using oxygen only. We have evaluated critical biochemical parameters such as levels of transaminase , cholinesterase , super - oxide dismutase, catalase , phospholipase A, calcium dependent ATPase, reduced glutathione , glucose 6 phosphate dehydrogenase and lipid peroxidation. Interestingly, in spite of CCl_4 adminis tration, group 3 did not differ from group 1, while groups 2 and 4 showed significant differences from groups 1 and 3 and displayed hepatic damage . To our knowledge these are the first experimental results showing that repeated administration of ozone in atoxic doses is able to induce an adaptation to oxidative stress thus enabling the animals to maintain hepatocellular integrity after CCl_4 poisoning.
4-Hydroxynonenal (HNE) is one of the major end products of lipid peroxidation and may have either physiological or pathological significance regulating cell proliferation. We studied some biochemical ...effects of HNE, at various concentrations (0.1-100 M), on Jurkat T cells incubated thereafter for 24, 48 and 72 h. HNE at low concentrations significantly enhanced the proliferation index, whereas at higher concentrations progressively blocked cell proliferation. Caspase 3 activity increased significantly at HNE concentrations between 1 and 10 M and decreased at higher concentrations. Superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione reductase (GSH-Rd) increased progressively with HNE concentrations, particularly GSH-Px. Glucose-6-phosphate dehydrogenase (G6PDH) showed a different pattern, increasing at low HNE (1-5 M) concentrations and rapidly declined thereafter. These results show that HNE may induce growth inhibition of Jurkat T cells and regulate the activity of typical antioxidant enzymes. Furthermore, the protective effect of doubling the foetal calf serum still points out the risk that cultured cells undergo oxidative stress during incubation.
The aim of this review is to elucidate the biochemical, molecular, immunological, and pharmaceutical mechanisms of action of ozone dissolved in biological fluids. Studies performed during the last ...two decades allow the drawing of a comprehensive framework for understanding and recommending the integration of ozone therapy for spinal pain.
An in-depth screening of primary sources of information online - via SciFinder Scholar, Google Scholar, and Scopus databases as well as Embase, PubMed, and the Cochrane Database of Systemic Reviews - was performed. In this review, the most significant papers of the last 25 years are presented and their proposals critically evaluated, regardless of the bibliometric impact of the journals.
The efficacy of standard treatments combined with the unique capacity of ozone therapy to reactivate the innate antioxidant system is the key to correcting the oxidative stress typical of chronic inflammatory diseases. Pain pathways and control systems of algesic signals after ozone administration are described.
This paper finds favors the full insertion of ozone therapy into pharmaceutical sciences, rather than as either an alternative or an esoteric approach.
The trigger system of the KLOE experiment Adinolfi, M; Ambrosino, F; Antonelli, M ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
10/2002, Letnik:
492, Številka:
1
Journal Article
Recenzirano
Odprti dostop
We present the design of the trigger system for the KLOE experiment at the Frascati
φ-factory DAΦNE. The detector consists of a large-volume drift chamber and a calorimeter both immersed in a
0.52
T
...solenoidal field. The trigger, structured with a first- and a second-decision level, is based on the multiplicity of energy deposits in the calorimeter and of hits in the drift chamber. The selection criteria are described and the efficiency for detecting
φ decays is evaluated using data.
OZONE (O_3) is a controversial gas because, owing to its potent oxidant properties, it exerts damaging effects on the respiratory tract and yet it has been used for four decades as a therapy. While ...the disinfectant activity of O_3 is understandable, it is less clear how other biological effects can be elicited in human blood with practically no toxicity. On the other hand plasma and cells are endowed with a powerful antioxidant system so that a fairly wide range of O_3 concentrations between 40 and 80μ g/ml per gram of blood (˜0.83-1.66 mM) are effective but not deleterious. After blood ozonation total antioxidant status (TAS) and plasma protein thiol groups (PTG) decrease by 20% and 25%, respectively, while thiobarbituric acid reactive substances (TBARS) increases up to fivefold. The increase of haemolysis is negligible suggesting that the erythrocyte membrane is spared at the expense of other sacrificial substrates. While there is a clear relationship between the ozone dose and IL-8 levels, we have noticed that high TAS and PTG values inhibit the cytokine production. This is in line with the current idea that hydrogen peroxide, as a byproduct of O_3 decomposition, acts as a messenger for the cytokine induction.
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