From strengthening the construction of an innovation system, adhering to the green development model of the industry, advancing the rural revitalization strategy, strengthening the protection and ...inheritance of cultural heritage, accelerating the development of social undertakings, and expanding the space for regional cooperation, this paper discusses the strategies for the construction of sustainable development experimental zone in Fumian District, Yulin City. It is intended to build Fumian District into a vibrant, economically prosperous, urban and rural integrated, characteristic cultural, safe and harmonious, inclusive and open district.
This paper first analyzes the main opportunities for sustainable development of Fumian District, Yulin City, and then discusses the challenges faced by Fumian District in its sustainable development. ...Finally, it proposes safeguard measures for sustainable development of Fumian District: (i) strengthening leadership and implementing responsibilities; (ii) enhancing cooperation and gathering forces; (iii) reinforcing supervision and ensuring implementation; (iv) increasing input and integrating resources; (v) strengthening publicity and creating an atmosphere.
Obesity is a strong risk factor for many diseases, with controversy regarding the cause(s) of tuberculosis (TB) reflected by contradictory findings. Therefore, a larger sample population is required ...to determine the relationship between obesity and TB, which may further inform treatment.
Obesity-related indicators and TB mutation data were obtained from a genome-wide association study database, while representative instrumental variables (IVs) were obtained by screening and merging. Causal relationships between exposure factors and outcomes were determined using two-sample Mendelian randomization (MR) analysis. Three tests were used to determine the representativeness and stability of the IVs, supported by sensitivity analysis.
Initially, 191 single nucleotide polymorphisms were designated as IVs by screening, followed by two-sample MR analysis, which revealed the causal relationship between waist circumference odds ratio (OR): 2.13 (95% confidence interval (CI): 1.19-3.80); p = 0.011 and TB. Sensitivity analysis verified the credibility of the IVs, none of which were heterogeneous or horizontally pleiotropic.
The present study determined the causal effect between waist circumference and TB by two-sample MR analysis and found both to be likely to be potential risk factors.
Background and Aims
Coronavirus disease 2019 (COVID‐19) is a new infectious disease. To reveal the hepatic injury related to this disease and its clinical significance, we conducted a multicenter ...retrospective cohort study that included 5,771 adult patients with COVID‐19 pneumonia in Hubei Province.
Approach and Results
We reported the distributional and temporal patterns of liver injury indicators in these patients and determined their associated factors and death risk. Longitudinal liver function tests were retrospectively analyzed and correlated with the risk factors and death. Liver injury dynamic patterns differed in alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (TBIL). AST elevated first, followed by ALT, in severe patients. ALP modestly increased during hospitalization and largely remained in the normal range. The fluctuation in TBIL levels was mild in the non‐severe and the severe groups. AST abnormality was associated with the highest mortality risk compared with the other indicators of liver injury during hospitalization. Common factors associated with elevated liver injury indicators were lymphocyte count decrease, neutrophil count increase, and male gender.
Conclusion
The dynamic patterns of liver injury indicators and their potential risk factors may provide an important explanation for the COVID‐19‐associated liver injury. Because elevated liver injury indicators, particularly AST, are strongly associated with the mortality risk, our study indicates that these parameters should be monitored during hospitalization.
The flavonoid extract from Erigeron breviscapus, breviscapine, has increasingly been used to treat cardio- and cerebrovascular diseases in China for more than 30 years, and plant supply of E. ...breviscapus is becoming insufficient to satisfy the growing market demand. Here we report an alternative strategy for the supply of breviscapine by building a yeast cell factory using synthetic biology. We identify two key enzymes in the biosynthetic pathway (flavonoid-7-O-glucuronosyltransferase and flavone-6-hydroxylase) from E. breviscapus genome and engineer yeast to produce breviscapine from glucose. After metabolic engineering and optimization of fed-batch fermentation, scutellarin and apigenin-7-O-glucuronide, two major active ingredients of breviscapine, reach to 108 and 185 mg l
, respectively. Our study not only introduces an alternative source of these valuable compounds, but also provides an example of integrating genomics and synthetic biology knowledge for metabolic engineering of natural compounds.
•Two mechanisms are responsible for keyhole-induced bubble formation.•The pressure balance equation is proposed to analyze the breaking of molten bridge by laser beam.•Large bubble and small bubble ...have high coalescence efficiency.
Laser welding experiment with the aid of glass, and numerical simulation are carried out to study the keyhole behavior and keyhole-induced bubble formation. Two mechanisms are responsible for keyhole-induced bubble formation. The first mechanism is the strong fluid flow inside the weld pool, and the capillary instability of the whole keyhole, causing the collapse between rear keyhole wall and front keyhole wall. This mechanism contributes to most of keyhole-induced bubble formation. The second mechanism is the instability of the rear keyhole wall caused by the increase absorption of laser energy reflected by the bulge at front keyhole wall. The breaking of molten bridge is analyzed based on static pressure balance. The molten bridge with large curvature and low temperature is difficult to be broken. Bubble coalescence can be clearly observed at the bottom of the weld pool. Large bubble and small bubble have high coalescence efficiency. The bubbles at keyhole bottom have more time to escape without broken by laser beam, and the bottom part of rear keyhole wall is more easily depressed, so bubbles are easily formed at the keyhole bottom.
Kaempferol is a flavonol with broad bioactivity of anti-oxidant, anti-cancer, anti-diabetic, anti-microbial, cardio-protective and anti-asthma. Microbial synthesis of kaempferol is a promising ...strategy because of the low content in primary plant source.
In this study, the biosynthesis pathway of kaempferol was constructed in the budding yeast Saccharomyces cerevisiae to produce kaempferol de novo, and several biological measures were taken for high production.
Firstly, a high efficient flavonol synthases (FLS) from Populus deltoides was introduced into the biosynthetic pathway of kaempferol. Secondly, a S. cerevisiae recombinant was constructed for de novo synthesis of kaempferol, which generated about 6.97 mg/L kaempferol from glucose. To further promote kaempferol production, the acetyl-CoA biosynthetic pathway was overexpressed and p-coumarate was supplied as substrate, which improved kaempferol titer by about 23 and 120%, respectively. Finally, a fed-batch process was developed for better kaempferol fermentation performance, and the production reached 66.29 mg/L in 40 h.
The titer of kaempferol in our engineered yeast is 2.5 times of the highest reported titer. Our study provides a possible strategy to produce kaempferol using microbial cell factory.
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