In Italy 1.5 Tg dry matter of residues are estimated to be produced by the agri-food sector. Approximately 30% of them are represented by residues of wine industry sector: grape marc. Referring to ...its production, it is possible to evaluate about 10 Mg of grapes from each vineyard hectare that generate, as wine industry residue, 2.7 Mg of grape marc, corresponding to about 19 GJ in terms of energy content. This kind of biomass is heterogenous and composed of stalks, grape skins and seeds. In this paper, in order to investigate the possibility of an energy and industrial utilization, the physical-chemical characteristics of each single component of grape marc are examined. In addition, a mechanical extraction test on the seed was performed to evaluate the vegetable oil production and the characteristics of the cake. Results on grape marc components put in evidence some difference in terms of ash and chemical elements content, which represent, specifically for these materials, the most critical aspects to take into account in combustion heating systems.
► Physical-chemical characteristics of the single component of grape marc have been studied. ► We carried out analyzes on stalks, grape skins, seeds, oikcakes and extraction meals. ► We also evaluated the grapeseed oil production. ► There are some differences in terms of ash and some chemical element among grape marc components. ► The separation in different components can be useful for grape marc valorization.
During the past four years, the European wood pellet market is currently continuing to increase. Total European consumption was about 9.8 Tg and Italian market reaches almost 1 Tg mainly for domestic ...supply. The present investigation provides an evaluation of pellet quality of Italian market by analysis of 88 wood pellet samples acquired randomly and directly from the sale points. Particular attention has been dedicated to relationship between ash and some elements. Results highlight that only half of samples fulfil the A1 class (best quality) requirements established in the EN 14961-2. Statistical analysis pointed out that ash content is mainly linked to sulphur, potassium and chlorine. The results show that low limit threshold values for ash content ensure low chlorine and sulphur contents.
•Several pellet samples were collected directly in the market and analysed.•EN 14961-2 standard defines pellet quality.•Pellet quality is important for small domestic applications like boilers and stoves.•Only a half of the samples fulfil the better quality class requirements.•Ash content is mainly linked to sulphur, potassium and chlorine.
Acute large-bowel obstruction represents a surgical challenge whose management is controversial, usually requiring emergent surgery in adverse circumstances with increased morbidity and mortality ...compared with elective situations.
We report our experience with self-expanding metal stents in the initial management of acute neoplastic colorectal obstruction. From December 2000 to February 2003, insertion of self-expanding metal stents under endoscopic and fluoroscopic guidance was attempted in 22 patients, in 9 as primary palliative measure (group A) whereas in 13 as a bridge to surgery (group B).
Stenting was technically successful in 20 patients. There were 2 perforations, and one patient died as a consequence. Nine of the 11 patients in the group B underwent elective colonic resection while in two patients with advanced malignancy the stent was considered as a definitive palliative treatment. In all 10 patients with ultimate prosthesis (8 group A, 2 group B) two dislocations and one recurrent obstruction were noted in the mean follow-up of 12 months.
Self expanding metal stents represent a good option in the treatment of large bowel obstruction, providing time for a complete preoperative evaluation and mechanical bowel preparation as well as for restoring patient's general conditions thus avoiding emergency surgery with considerably lower morbidity and mortality rates. In patients with advanced cancer, they provide an alternative to surgery with satisfactory results.
In the treatment of gastric cancer total gastrectomy (TG) is one of the most important operation and the esophagojejunal anastomotic leakage is the most important early complication. In our series of ...172 consecutive TGs (all controlled with Gastrografin-Rx in fifth postoperative day) we did not have any anastomotic fistula, independently from age, stage, type of limphadenectomy and visceral demolition. We believe that the correct technical performance is the most important factor in the esophagojejunal anastomosis.
The extracellular DNA pool in marine sediments is the largest reservoir of DNA of the world oceans and it potentially represents an archive of genetic information and gene sequences involved in ...natural transformation processes. However, no information is at present available for the gene sequences contained in the extracellular DNA and for the factors that influence their preservation. In the present study, we investigated the depurination and degradation rates of extracellular DNA in a variety of marine sediment samples characterized by different ages (up to 10 000 years) and environmental conditions according to the presence, abundance and diversity of prokaryotic gene sequences. We provide evidence that depurination of extracellular DNA in these sediments depends upon the different environmental factors that act synergistically and proceeds at much slower rates than those theoretically predicted or estimated for terrestrial ecosystems. These findings suggest that depurination in marine sediments is not the main process that limits extracellular DNA survival. Conversely, DNase activities were high suggesting a more relevant role of biologically driven processes. Amplifiable prokaryotic 16S rDNA sequences were present in most benthic systems analysed, independent of depurination and degradation rates and of the ages of the sediment samples. Additional molecular analyses revealed that the extracellular DNA pool is characterized by relatively low-copy numbers of prokaryotic 16S rDNA sequences that are highly diversified. Overall, our results suggest that the extracellular DNA pool in marine sediments represents a repository of genetic information, which can be used for improving our understanding of the biodiversity, functioning and evolution of ecosystems over different timescales.
The authors have conducted a retrospective study of surgical therapy by bilio-digestive anastomosis of benign and malignant diseases of the common bile duct. They conclude that this procedure should ...only be used when the anatomical and clinical conditions do not allow other surgical techniques to be employed.
Environmental DNA (eDNA) metabarcoding (parallel sequencing of DNA/RNA for identification of whole communities within a targeted group) is revolutionizing the field of aquatic biomonitoring. To date, ...most metabarcoding studies aiming to assess the ecological status of aquatic ecosystems have focused on water eDNA and macroinvertebrate bulk samples. However, the eDNA metabarcoding has also been applied to soft sediment samples, mainly for assessing microbial or meiofaunal biota. Compared to classical methodologies based on manual sorting and morphological identification of benthic taxa, eDNA metabarcoding offers potentially important advantages for assessing the environmental quality of sediments. The methods and protocols utilized for sediment eDNA metabarcoding can vary considerably among studies, and standardization efforts are needed to improve their robustness, comparability and use within regulatory frameworks. Here, we review the available information on eDNA metabarcoding applied to sediment samples, with a focus on sampling, preservation, and DNA extraction steps. We discuss challenges specific to sediment eDNA analysis, including the variety of different sources and states of eDNA and its persistence in the sediment. This paper aims to identify good-practice strategies and facilitate method harmonization for routine use of sediment eDNA in future benthic monitoring.
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•eDNA metabarcoding is revolutionizing the field of aquatic biomonitoring.•The methods and protocols for sediment eDNA metabarcoding can vary considerably.•Available information on metabarcoding applied to sediment samples were reviewed.•Challenges specific to sediment eDNA analysis were discussed.•The aim was to define best-practices and recommend potential standard procedures.
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