Summary
Eleven thousand, three hundred and seventy enhancer/promoter trap lines in
Arabidopsis
were generated via T‐DNA transformation utilizing the binary vector pD991 that contains a minimal ...promoter fused to the
uidA
reporter gene. Overall 31% of the lines generated exhibit a staining pattern in the inflorescence. Flanking DNA has been cloned from 15 lines exhibiting inflorescence staining patterns by either thermal asymmetric interlaced PCR (TAIL–PCR), inverse PCR (IPCR), or partial library construction. Seeds from these lines are available from the ABRC and NASC
Arabidopsis
stock centers and DNA pools are available from the ABRC.
In yeast and animals, cyclins have been demonstrated to be important regulators of cell cycle progression. In recent years, a large number of A-, B-, and D-class cyclins have been isolated from a ...variety of plant species. One class of cylins, the D-class cyclins, is important for progression through G1 phase of the cell cycle. In Arabidopsis, four D-class cyclins have been isolated and characterized (CYCLIN-D1;1, CYCLIN-D2;1, CYCLIN-D3;1, and CYCLIN-D4;1). In this report we describe the characterization of a fifth D-class cyclin gene, CYCLIN-D3;2 (CYCD3;2), from Arabidopsis. An enhancer trap line, line 5580, contains a T-DNA insertion in CYCD3;2. Enhancer trap line 5580 exhibits expression in young vegetative and floral primordia. In line 5580, T-DNA is inserted in the first exon of the CYCD3;2 gene; in homozygous 5580 plants CYCD3;2 RNA is not detectable. Even though CYCD3;2 gene function is eliminated, homozygous 5580 plants do not exhibit an obvious growth or developmental phenotype. Via in situ hybridization we demonstrate that CYCD3;2 RNA is expressed in developing vegetative and floral primordia. In addition, CYCD3;2 is also capable of rescuing a yeast strain that is deficient in G1 cyclin activity.
Viruses are obligate parasites as they require the machinery of the host cell to replicate. Inhibition of host factors co-opted during active infection is a strategy to suppress viral replication and ...a potential pan antiviral therapy. To define the cellular proteins and processes required for a virus during infection is thus crucial to understanding the mechanisms of virally induced disease. In this report, we generated fully infectious tagged influenza viruses and used infection-based proteomics to identify pivotal arms of cellular signaling required for influenza virus growth and infectivity. Using mathematical modeling, genetic, and pharmacologic approaches, we revealed that modulation of Sec61-mediated cotranslational translocation selectively impaired glycoprotein proteostasis of influenza as well as HIV and dengue viruses, and led to inhibition of viral growth and infectivity. Thus, by studying virus-human protein-protein interactions in the context of active replication we have identified targetable host factors for broad-spectrum antiviral therapies.
In yeast and animals, cyclins have been demonstrated to be
important regulators of cell cycle progression. In recent years, a
large number of A-, B-, and D-class cyclins have been isolated from a
...variety of plant species. One class of cyclins, the D-class cyclins, is
important for progression through G1 phase of the cell cycle. In
Arabidopsis, four D-class cyclins have been isolated and characterized
(
CYCLIN-D1;1
,
CYCLIN-D2;1
,
CYCLIN-D3;1
, and
CYCLIN-D4;1
). In this
report we describe the characterization of a fifth D-class cyclin gene,
CYCLIN-D3;2
(
CYCD3;2
), from Arabidopsis.
An enhancer trap line, line 5580, contains a T-DNA insertion in
CYCD3;2.
Enhancer trap line 5580 exhibits expression in
young vegetative and floral primordia. In line 5580, T-DNA is inserted
in the first exon of the
CYCD3;2
gene; in homozygous
5580 plants
CYCD3;2
RNA is not detectable. Even though
CYCD3;2
gene function is eliminated, homozygous 5580
plants do not exhibit an obvious growth or developmental phenotype. Via
in situ hybridization we demonstrate that
CYCD3;2
RNA is
expressed in developing vegetative and floral primordia. In addition,
CYCD3;2
is also capable of rescuing a yeast strain that
is deficient in G1 cyclin activity.
In yeast and animals, cyclins have been demonstrated to be important regulators of cell cycle progression. In recent years, a large number of A-, B-, and D-class cyclins have been isolated from a ...variety of plant species. One class of cyclins, the D-class cyclins, is important for progression through G1 phase of the cell cycle. In Arabidopsis, four D-class cyclins have been isolated and characterized (CYCLIN-D1; 1, CYCLIN-D2; 1, CYCLIN-D3; 1, and CYCLIN-D4; 1). In this report we describe the characterization of a fifth D-class cyclin gene, CYCLIN-D3; 2 (CYCD3; 2), from Arabidopsis. An enhancer trap line, line 5580, contains a T-DNA insertion in CYCD3; 2. Enhancer trap line 5580 exhibits expression in young vegetative and floral primordia. In line 5580, T-DNA is inserted in the first exon of the CYCD3; 2 gene; in homozygous 5580 plants CYCD3; 2 RNA is not detectable. Even though CYCD3; 2 gene function is eliminated, homozygous 5580 plants do not exhibit an obvious growth or developmental phenotype. Via in situ hybridization we demonstrate that CYCD3; 2 RNA is expressed in developing vegetative and floral primordia. In addition, CYCD3; 2 is also capable of rescuing a yeast strain that is deficient in G1 cyclin activity.
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