Human neurocysticercosis (NCC) is a worldwide neglected disease caused by Taenia solium metacestode and responsible for various complications and neurological disorders. This study aimed to evaluate ...the use of specific immunoglobulin Y (IgY) produced by laying hens immunized with a hydrophobic fraction of Taenia crassiceps metacestodes (hFTc) in NCC diagnosis. Egg yolk IgY antibodies were fractionated, purified and characterized. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the production kinetics and avidity maturation of anti-hFTc IgY antibodies throughout the IgY obtention process. Antigen recognition tests were carried out by Western blotting and immunofluorescence antibody test using purified and specific anti-hFTc IgY antibodies for detection of parasitic antigens of T. crassiceps and T. solium metacestodes. Sandwich ELISA was performed to detect circulating immune complexes formed by IgG and parasitic antigens in human sera. The results showed high diagnostic values (93.2% sensitivity and 94.3% specificity) for immune complexes detection in human sera with confirmed NCC. In conclusion, specific IgY antibodies produced from immunized hens with hFTc antigens were efficient to detect T. solium immune complexes in human sera, being an innovative and potential tool for NCC immunodiagnosis.
The expected global sea level rise by the year 2100 will determine adaptation of the whole coastal system and the land retreat of the shoreline. Future scenarios coupled with the improvement of ...mining technologies will favour increased exploitation of sand deposits for nourishment, especially for urban beaches and sandy coasts with lowlands behind them. The objective of the work is to provide useful tools to support planning in the management of sand deposits located on the continental shelf of Western Sardinia (western Mediterranean Sea). The work has been realised through the integration of data and information collected during several projects. Available data consist of morpho-bathymetric data (multibeam) associated with morphoacoustic (backscatter) data, collected in the depth range -25 to -700 m. Extensive coverage of high-resolution seismic profiles (Chirp 3.5 kHz) has been acquired along the continental shelf. Also, surface sediment samples (Van Veen grab and box corer) and vibrocorers have been collected. These data allow mapping of the submerged sand deposits with the determination of their thickness and volume and their sedimentological characteristics. Furthermore, it is possible to map the seabed geomorphological features of the continental shelf of Western Sardinia. All the available data (https://doi.org/10.1594/PANGAEA.895430) have been integrated and organised in a geodatabase implemented through a GIS and the software suite Geoinformation Enabling ToolkIT StarterKit.sup.® (GET-IT), developed by researchers of the Italian National Research Council for RITMARE project. GET-IT facilitates the creation of distributed nodes of an interoperable spatial data infrastructure (SDI) and enables unskilled researchers from various scientific domains to create their own Open Geospatial Consortium (OGC) standard services for distributing geospatial data, observations and metadata of sensors and data sets.
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