Allergic contact dermatitis (ACD) is an inflammatory skin reaction whose incidence has increased and has been associated with a dietary pattern rich in saturated fats and refined sugars. Considering ...the increased incidence of ACD and the lack of research about the influence of a short-term high-sugar diet on dermatitis, our aim is to improve understanding of the influence of a high-sugar diet on ACD. We introduced a diet rich in sugar fifteen days before inducing contact dermatitis with oxazolone, in mice, and maintained it until the end of the experiment, which lasted three weeks in total. The dermatitis model increased cholesterol and triglycerides in the liver, and the combination of diet and dermatitis increased weight and worsened liver cholesterol measurements. Furthermore, the high-sugar diet increased the production of IL-6, IFN-γ and TNF-α in the skin, which may be involved in the increase in epithelial skin thickness observed in experimental ACD.
The phenotypes of allergic airway diseases are influenced by the interplay between host genetics and the gut microbiota, which may be modulated by probiotics. We investigated the probiotic effects on ...allergic inflammation in A/J and C57BL/6 mice. C57BL/6 mice had increased gut microbiota diversity compared to A/J mice at baseline. Acetate producer probiotics differentially modulated and altered the genus abundance of specific bacteria, such as Akkermansia and Allistipes, in mouse strains. We induced airway inflammation followed by probiotic treatment and found that only A/J mice exhibited decreased inflammation, and the beneficial effects of probiotics in A/J mice were partially due to acetate production. To understand the relevance of microbial composition colonization in the development of allergic diseases, we implanted female C57BL/6 mice with A/J embryos to naturally modulate the microbial composition of A/J mice, which increased gut microbiota diversity and reduced eosinophilic inflammation in A/J. These data demonstrate the central importance of microbiota to allergic phenotype severity. Video Abstract.
RAW_REF_TEXT Correction /RAW_REF_TEXT RAW_REF_TEXT Open Access /RAW_REF_TEXT RAW_REF_TEXT Published:14 July 2021 /RAW_REF_TEXT Correction to: A probiotic has differential effects on allergic airway ...inflammation in A/J and C57BL/6 mice and is correlated with the gut microbiome RAW_REF_TEXT Mateus B. Casaro1, /RAW_REF_TEXT RAW_REF_TEXT Andrew M. Thomas2,3,4, /RAW_REF_TEXT RAW_REF_TEXT Eduardo Mendes1, /RAW_REF_TEXT RAW_REF_TEXT Claudio Fukumori1, /RAW_REF_TEXT RAW_REF_TEXT Willian R. Ribeiro1, /RAW_REF_TEXT RAW_REF_TEXT Fernando A. Oliveira5, /RAW_REF_TEXT RAW_REF_TEXT Amanda R. Crisma6, /RAW_REF_TEXT RAW_REF_TEXT Gilson M. Murata7, /RAW_REF_TEXT RAW_REF_TEXT Bruna Bizzarro8, /RAW_REF_TEXT RAW_REF_TEXT Anderson Sá-Nunes8, /RAW_REF_TEXT RAW_REF_TEXT Joao C. Setubal4, /RAW_REF_TEXT RAW_REF_TEXT Marcia P. A. Mayer9, /RAW_REF_TEXT RAW_REF_TEXT Flaviano S. Martins10, /RAW_REF_TEXT RAW_REF_TEXT Angélica T. Vieira11, /RAW_REF_TEXT RAW_REF_TEXT Ana T. F. B. Antiorio12, /RAW_REF_TEXT RAW_REF_TEXT Wothan Tavares-de-Lima13, /RAW_REF_TEXT RAW_REF_TEXT Niels O. S. Camara8, /RAW_REF_TEXT RAW_REF_TEXT Rui Curi14, /RAW_REF_TEXT RAW_REF_TEXT Emmanuel Dias-Neto3,15 & /RAW_REF_TEXT RAW_REF_TEXT Caroline M. Ferreira 1 /RAW_REF_TEXT Microbiome volume 9, Article number: 159 (2021) Cite this article RAW_REF_TEXT 89 Accesses /RAW_REF_TEXT RAW_REF_TEXT 1 Altmetric /RAW_REF_TEXT RAW_REF_TEXT Metrics details /RAW_REF_TEXT The Original Article was published on 10 June 2021 Correction to: Fig. 4 figure1 A/J embryo implantation in C57BL/6 mothers resulted in experimental allergic airway inflammation similar to C57BL/6 offspring. a Schematic representation of the embryo transfer and OVA-inducing airway inflammation protocol. b Phylogenetic diversity in mice. All values were calculated using Adonis with 999 permutations on unweighted UniFrac distances. d Total and differential (Eos, eosinophils; Neut, neutrophils; Mono, mononuclear) number of cells in the bronchoalveolar lavage (BALF) of the A/J, C57BL/6, and A/J transplanted OVA groups (n = 3–5). e Levels of (pg/ml) of interleukin (IL)-4 and interferon (INF)-γ in the BALF of the A/J, C57BL/6, and A/J transplanted OVA groups (n = 3–5). Correction Open Access Published:14 July 2021 /RAW_REF_TEXT Correction to: A probiotic has differential effects on allergic airway inflammation in A/J and C57BL/6 mice and is correlated with the gut microbiome RAW_REF_TEXT Mateus B. Casaro1, Andrew M. Thomas2,3,4, Eduardo Mendes1, Claudio Fukumori1, Willian R. Ribeiro1, Fernando A. Oliveira5, Amanda R. Crisma6, Gilson M. Murata7, Bruna Bizzarro8, Anderson Sá-Nunes8, Joao C. Setubal4, Marcia P. A. Mayer9, Flaviano S. Martins10, Angélica T. Vieira11, Ana T. F. B. Antiorio12, Wothan Tavares-de-Lima13, Niels O. S. Camara8, Rui Curi14, Emmanuel Dias-Neto3,15 & Caroline M. Ferreira 1 /RAW_REF_TEXT Microbiome volume 9, Article number: 159 (2021) Cite this article RAW_REF_TEXT 89 Accesses 1 Altmetric Metrics details
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•Maternal synbiotics modulate gut microbiota depending on host genetics.•Maternal synbiotics affect allergic outcomes in A/J and B6 offspring differently.•Microbiota diversity was not ...enough to regulate airway eosinophilia in allergic A/J mice.
Emerging evidence has shown that the maternal diet influences the microbiota. Our objective was to elucidate whether synbiotic consumption during the perinatal period equally modulates offspring gut microbiota in the A/J and C57BL/6 and affects airway inflammatory cell infiltration. We administered the synbiotic to the dam throughout the whole perinatal period. To analyze the influence of gut microbiota changes on offspring health, we induced allergic airway inflammation on offspring. Twenty-four hours after the last ovalbumin challenge, pulmonary inflammation was analyzed. Perinatal synbiotic supplementation reduced eosinophilic inflammation and mucus production only in the C57BL/6 offspring. Synbiotic supplementation during the perinatal period increased the Lactobacillus genera, which could be associated with the immune regulatory effects observed in the C57BL/6 offspring. Interestingly, synbiotic supplementation increased the diversity of gut microbiota in the A/J offspring without improving airway inflammation, indicating that diversity itself was not sufficient to regulate experimental lung inflammation.
Description of Ovariectomy Protocol in Mice Souza, Vanessa R; Mendes, Eduardo; Casaro, Mateus ...
Methods in molecular biology (Clifton, N.J.),
2019, Letnik:
1916
Journal Article
Estrogen and ovarian function decline are relevant characteristics of menopause period. Numerous physiological, metabolic and immunological alterations in the female body occur in the menopause ...period and some of these changes remain uncertain. The animal model that mimics menopause phase is an important approach to better comprehend the biological process involved in this period of women life. Ovariectomy is a procedure where ovaries are surgically excised and have been a valuable tool for understanding estrogen deficiency through animal experiments. Despite the diversity of ovariectomy protocols, the aim of this chapter is to provide a comprehensive guideline in performing ovariectomy in mice. Furthermore, isoflurane anesthesia system, postoperative care and surgery success evaluation will be described. We highlight that all procedures must be carried out by a qualified and trained professional, respecting ethical and safety principles.
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