Using two-phase flow boiling heat transfer theory, the RPI subcooling boiling heat transfer model was established to study the temperature rise characteristics of the permanent magnet synchronous ...motor (PMSM) of electric vehicles under peak operating conditions, and the effects of coolant inlet temperature, altitude and inlet flow rate on the motor temperature rise were analyzed. The results showed that: the temperature rise characteristics of the motor are closer to the test results when boiling heat transfer is considered after the motor is warmed up, so the effect of boiling heat transfer of the cooling system should be considered when studying the temperature rise characteristics of the motor; The temperature rise characteristic of the motor increases with the increase of coolant inlet temperature at peak working condition. The short time required for the motor winding to reach 150 °C indicates that the motor temperature rises quickly. In the plateau environment, the temperature growth rate of the motor at peak working conditions increases with the increase of cooling water inlet temperature, while the motor temperature decreases with the decrease of atmospheric pressure. Thus, due to the boiling heat transfer phenomenon of cooling water two-phase flow, the temperature rise characteristic of the motor at high altitude is better than that in plain area.
p-Aminophenol was synthesized by catalytic hydrogenation of
p-nitrophenol on nano-sized nickel catalysts prepared by a chemical reduction method from aqueous solutions. The catalysts were ...characterized by XRD, EDS, SEM, HRTEM and Mastersizer 2000. Analysis results show that as-prepared catalysts are pure f.c.c. nickel and are prone to aggregation; the average particle size of nickel catalysts is 57
nm and there are high-density defects on particle surfaces. In hydrogenation reactions of
p-nitrophenol, the hydrogenation rate is zero-order dependent on nitro aromatics and increases with increasing of hydrogen pressure. Compared with commercial Raney Ni, catalytic properties (activity, selectivity, and stability) of the as-prepared nickel are superior. The reason proposed for higher catalytic activity of nano-sized nickel is a combination effect of the small particle size and high-density surface defects. The partial sintering of nano-sized nickel might lead to the deactivation of the catalytic activity of nano-sized nickel.
Ewing's sarcoma (ES) is a rare and highly aggressive malignant tumor arising from bone and soft tissue. Suffering from intractable or recurrent diseases, the patients' therapy options are very ...limited. It is extremely urgent to identify novel potential therapeutic targets for ES and put them into use in clinical settings. In the present study, high-throughput screening of a small molecular pharmacy library was performed. The killing effect of the Aurora kinase A (AURKA) inhibitor TCS7010 in ES cells was identified, and AURKA was selected as the research object for further study. Disparate suppressants were adopted to study the cell death manner of TCS7010. TCS7010 and RNA silencing were used to evaluate the functions of AURKA in the apoptosis and ferroptosis of ES cells. Co-immunoprecipitation assay was used to investigate the correlation of AURKA and nucleophosmin1 (NPM1) in ES. Nude-mice transplanted tumor model was used for investigating the role of AURKA in ES in vivo. Investigations into the protein activities of AURKA were conducted using ES cell lines and xenograft models. AURKA was found to be prominently upregulated in ES. The AURKA expression level was remarkably connected to ES patients' shorter overall survival (OS) and event-free survival (EFS). Furthermore, AURKA inhibition markedly induced the apoptosis and ferroptosis of ES cells and attenuated tumorigenesis in vivo. On the part of potential mechanisms, it was found that AURKA inhibition triggered the apoptosis and ferroptosis of ES cells through the NPM1/Yes1 associated transcriptional regulator (YAP1) axis, which provides new insights into the tumorigenesis of ES. AURKA may be a prospective target for clinical intervention in ES patients.
Transparent superhydrophobic coatings, having high water contact angle (>160°), low sliding angle (<5°) and high transparency (transmittance closes to 90%), were prepared by using ...poly(methylhydrosiloxane) (PMHS) and tetraethoxysilane (TEOS) as precursors based on a simple sol–gel process. The high volume-fraction porosity structure and low surface energy are responsible for the superhydrophobicity of coating in this paper.
•Transparent superhydrophobic coatings were prepared by a simple sol–gel method.•High SSA and low surface energy built the superhydrophobicity of coating.•Appropriate surface area (SSA) achieves superior superhydrophobicity coating.•The nanocoating could switch from superhydrophobic to superhydrophilic by heating.
Transparent superhydrophobic coatings, having high water contact angle (>160°) and low sliding angle (<5°), were prepared by using poly(methylhydrosiloxane) (PMHS) and tetraethoxysilane (TEOS) as precursors based on a simple sol–gel process. The influence of different mass ratios of PMHS to TEOS on the transparency and superhydrophobicity of resulting coatings was investigated herein to get the optimum performance coating. The structure, composition and morphology of optimum performance coating were characterized by various technologies including Fourier transform infrared (FT-IR) spectroscopy, thermal analysis, BET, 29Si CP MAS NMR, X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). The optimum performance coating exhibits superhydrophobicity (CA, 164.7° and SA, 2.7°), a high transparency (transmittance closes to 90%) and a good thermal stability (up to 400°C). Note that the optimum coating directly from sol–gel process exhibits poor moisture resistance. The low surface energy and high volume-fraction porosity structure are responsible for the superhydrophobicity, transparency and thermal stability of the as-prepared coating, while the poor moisture is attributed to the untreated hydroxyl groups on the surface of coating. The moisture resistance of coating can be improved by further treated by cetyltrimethoxylsilane (CTMS), and the coating could switch from superhydrophobic (164.7°) to superhydrophilic (0°) after heat-treating at 600°C.
Glucosamine 6-phosphate N-acetyltransferase (GNPNAT1) is a crucial enzyme involving hexosamine biosynthesis pathway and is upregulated in breast cancer (BRCA). However, its biological function and ...mechanism on patients in BRCA have not been investigated.
In this study, the differential expression of GNPNAT1 was analyzed between BRCA tissues and normal breast tissues using the Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) database, which was validated by quantitative real-time polymerase chain reaction, Western blot and immunohistochemistry. Then, the potential clinical value of GNPNAT1 in BRCA was investigated based on TCGA database. Functional enrichment analyses, including Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, Gene Set Variation Analysis, were performed to explore the potential signaling pathways and biological functions involved in GNPNAT1 in BRCA. Tumor immune infiltration was analyzed using ESTIMATE, CIBERSORT and TISIDB database; and immune therapy response scores were assessed using TIDE. Finally, Western blot, Cell counting kit-8 and Transwell assay were used to determine the proliferation and invasion abilities of breast cancer cells with GNPNAT1 knockdown.
GNPNAT1 was up-regulated in BRCA tissues compared with normal tissues which was subsequently verified in different cell lines and clinical tissue samples. Based on TCGA and GEO, the overexpression of GNPNAT1 in BRCA contributed to a significant decline in overall survive and disease specific survive. Functional enrichment analyses indicated that the enriched pathways in high GNPNAT1 expression group included citrate cycle, N-glycan biosynthesis, DNA repair, and basal transcription factors. Moreover, the overexpression of GNPNAT1 was negatively correlated with immunotherapy response and the levels of immune cell infiltration of CD8+ T cells, B cells, natural killer cells, dendritic cells and macrophages. Knockdown of GNPNAT1 impairs the proliferation and invasion abilities of breast cancer cells.
GNPNAT1 is a potential diagnostic, prognostic biomarker and novel target for intervention in BRCA.
Seismic blind high-resolution inversion (BHRI) aims at retrieving the high-resolution data to characterize the stratigraphic structures in the case of an unknown seismic wavelet. However, the unknown ...wavelet and ill-posedness pose a great challenge to the high-resolution inversion. Regularization-based BHRI is an effective approach. However, it is sensitive to the sets of initial values, regularization terms, and regularization parameters and suffers from computational burden problems. To address these issues, we propose a sequential iterative deep learning method (SIDLM) to implement a BHRI in a fast computational speed, which incorporates three learned components to sequentially invert initial high-resolution data, seismic wavelet, and final high-resolution data in an end-to-end fashion. Specifically, to mitigate the influence of initial values, a data-driven network U-Net is adopted to learn an initial high-resolution data. Furthermore, the architecture makes use of prior information encoded in the forward operator to build a new general alternating direction method of multipliers (ADMM)-like iterative deep neural network, instead of the traditional alternating iterative inversion. The proposed ADMM-like network utilizes the convolutional neural networks to learn the proximal operators to solve each subproblems in alternating iterative inversion. Therefore, all parameters of BHRI, such as the regularization parameters and transform operator, can be implicitly learned from the training datasets in an end-to-end fashion, not limited to the form of the penalty function. Finally, the synthetic and field data examples are conducted to demonstrate the effectiveness of the proposed SIDLM.
Abstract Ewing’s sarcoma (ES) represents a rare yet exceedingly aggressive neoplasm that poses a significant health risk to the pediatric and adolescent population. The clinical outcomes for ...individuals with relapsed or refractory ES are notably adverse, primarily attributed to the constrained therapeutic alternatives available. Despite significant advancements in the field, molecular pathology-driven therapeutic strategies have yet to achieve a definitive reduction in the mortality rates associated with ES. Consequently, there exists an imperative need to discover innovative therapeutic targets to effectively combat ES. To reveal the mechanism of the SETD8 (also known as lysine methyltransferase 5A) inhibitor UNC0379, cell death manners were analyzed with different inhibitors. The contributions of SETD8 to the processes of apoptosis and ferroptosis in ES cells were evaluated employing the histone methyltransferase inhibitor UNC0379 in conjunction with RNA interference techniques. The molecular regulatory mechanisms of SETD8 in ES were examined through the application of RNA sequencing (RNA-seq) and mass spectrometry-based proteomic analysis. Moreover, nude mouse xenograft models were established to explore the role of SETD8 in ES in vivo. SETD8, a sole nucleosome-specific methyltransferase that catalyzes mono-methylation of histone H4 at lysine 20 (H4K20me1), was found to be upregulated in ES, and its overexpression was associated with dismal outcomes of patients. SETD8 knockdown dramatically induced the apoptosis and ferroptosis of ES cells in vitro and suppressed tumorigenesis in vivo. Mechanistic investigations revealed that SETD8 facilitated the nuclear translocation of YBX1 through post-transcriptional regulatory mechanisms, which subsequently culminated in the transcriptional upregulation of RAC3 . In summary, SETD8 inhibits the apoptosis and ferroptosis of ES cells through the YBX1/RAC3 axis, which provides new insights into the mechanism of tumorigenesis of ES. SETD8 may be a potential target for clinical intervention in ES patients.
Open-globe injuries (OGIs) remain the important cause of visual impairment and loss in all ages. Computed Tomography (CT) is a useful and common tool in the evaluation of the injuries of the eyeball. ...Prognostic value of CT scan in OGIs has been evaluated in many studies. However, there is no published consistent systematic scoring method for CT scan in OGIs. The purpose of this study was to evaluate the CT characteristics of OGIs and build a scoring method according to the CT scans which may aid the clinicians in management of OGIs.
Retrospective chart review of inpatients with clinical diagnosis of OGIs between 2017 and 2021 at Department of Ophthalmology, Henan Eye Institute, Henan Eye Hospital, Henan provincial People's Hospital (Zhengzhou, China).
There were 1120 eyes from 1117 patients included in our study. The mean age was 35.7 ± 21.9 years with the range from 1 to 91 years. Significant male predominance was noted (889, 79.6%). CT scans of the OGIs were evaluated. Abnormality of anterior segment, posterior segment, and globe contour and volume were graded respectively. The most serious abnormality of anterior segment, posterior segment, and globe contour and volume were grade 3, 4 and 3 respectively and score 3, 4 and 3 respectively. Score of the CT scans of an open-injured globe ranged from 0 to 10. The correlation coefficient between the score and wound length was 0.798. The correlation coefficient between the score and final visual acuity was 0.799. In 78 eyes with 0 score, 70 eyes (89.7%) gained final visual acuity of 0.3 or better. In 31 eyes with 10 score, 20 eyes (64.5%) underwent evisceration of the eye globe and 10 eyes got visual acuity of no light perception and 1 eye lost to follow-up.
CT scans is a useful tool in evaluating the severity of an open-injured globe. Scoring of the CT scans of an open-injured globe is a meaningful attempt and it may provide useful prognostic information regarding the outcome of an open-injured globe.
•Higher step count is inversely associated with the risk of premature death and cardiovascular events.•As measured by accelerometers, 8959 steps/day (Q3) had a 40.36% lower risk of all-cause ...mortality than 4183 steps/day (Q1).•As measured by accelerometers, 9500 steps/day (Q3) had a 35.05% lower risk of cardiovascular events than 3500 steps/day(Q1).•These associations were in nonlinear dose–response patterns.
A goal of 10,000 steps per day is widely advocated, but there is little evidence to support that goal. Our purpose was to examine the dose–response relationships between step count and all-cause mortality and cardiovascular disease risk.
Cochrane Central Register of Controlled Trials, EMBASE, OVID, PubMed, Scopus, and Web of Science databases were systematically searched for studies published before July 9, 2021, that evaluated the association between daily steps and at least 1 outcome.
Sixteen publications (12 related to all-cause mortality, 5 related to cardiovascular disease; and 1 article contained 2 outcomes: both all-cause death and cardiovascular events) were eligible for inclusion in the meta-analysis. There was evidence of a nonlinear dose–response relationship between step count and risk of all-cause mortality or cardiovascular disease (p = 0.002 and p = 0.014 for nonlinearity, respectively). When we restricted the analyses to accelerometer-based studies, the third quartile had a 40.36% lower risk of all-cause mortality and a 35.05% lower risk of cardiovascular event than the first quartile (all-cause mortality: Q1 = 4183 steps/day, Q3 = 8959 steps/day; cardiovascular event: Q1 = 3500 steps/day, Q3 = 9500 steps/day; respectively).
Our meta-analysis suggests inverse associations between higher step count and risk of premature death and cardiovascular events in middle-aged and older adults, with nonlinear dose–response patterns.
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