To study the effects of interleukin-10 on hepatic stellate cells and liver tissue in experimental rats hepatic fibrosis.
Rat hepatic fibrosis model induced by carbon tetrachloride was established. ...Liver tissues were harvested from the rats administered CCl4 with or without IL-10 treatment and the animals of the control group. The expression of TGF-beta1, MMP-2 and TIMP-1 in the liver tissues was measured by S-P immunohistochemistry. In addition, another model was established; HSCs in rats in each group were isolated. RT-PCR was employed to analyze TGF-beta1, MMP-2 and TIMP-1 mRNA expression in cells and immunocytochemistry was performed to detect protein expression of alpha-SMA, NF-kappaB, TGF-beta1, MMP-2 and TIMP-1 in HSCs.
Rat hepatic fibrosis was developed successfully. The fibrosis changes were partially reversed by simultaneous administration of IL-10. The positive signals of TGF-beta1, MMP-2 and TIMP-1 were observed more frequently (P<0.05) in the CCl4-treated group compared to those in the IL-10-treated group and the control group. HSCs were successfully isolated. TGF-beta1, MMP-2 and TIMP-1 mRNA in HSCs increased obviously during the course of hepatic fibrosis, and their levels were decreased after the treatment with IL-10 (P<0.05). The immunocytochemistry positive levels for TGF-beta1, MMP-2, TIMP-1, alpha-SMA and NF-kappaB in the fibrogenesis group were increased significantly compared to the normal group (P<0.01). The positive signals decreased significantly (P<0.05) after the treatment with IL-10.
The expression of TGF-beta1, MMP-2 and TIMP-1 increased in liver or in HSC of hepatic fibrosis rats and decreased after treatment with IL-10. The IL-10 could inhibit the activation of HSCs and make an antifibrogenic process come into effect in this way.
12-Lipoxygenase (12-LOX) is over-expressed in a variety of human tumors, but its exact effect on the tumorogenesis of gastric cancer remains largely obscure. To investigate the effect of 12-LOX and ...its inhibitor baicalein on proliferation and apoptosis of human gastric cancer, AGS cells were separately treated with 12-hydroxyeicosatetraenoic acid (12-HETE, a metabolite of 12-LOX) and baicalein. MTT assay revealed that the absorbance of the 12-HETE-treated group was significantly (
P
< 0.01) higher than that of control group and that the absorbance of baicalein-treated group was significantly (
P
< 0.01) less than that of the control group, and that 48 h after treatment the apoptosis index of the baicalein-treated group was significantly (
P
< 0.01) higher than that of the untreated group and was significantly (
P
< 0.01) lower in the 12-HETE-treated group. Western blotting analysis was used to investigate the mechanism of these effects. The results revealed that the concentration of phosphorylated ERK in cells treated with 100 nmol L
−1
12-HETE was significantly (
P
< 0.05) higher than in the untreated group and that the concentration of phosphorylated ERK1/2 in cells treated with 40 μmol L
−1
baicalein was significantly (
P
< 0.05) lower than in the untreated group. The expression level of
bcl-2
was up-regulated and down-regulated after separate treatment with 12-HETE and baicalein, respectively, and both of these effects could be blocked by PD98059. Protein kinase C (PKC) activity was increased by treatment with 12-HETE and reduced by treatment with baicalein (
P
< 0.05). The PKC inhibitor BIM (bisindolymaleimide-I) blocked the phosphorylation of ERK1/2 and activation of PKC induced by 12-LOX. When pretreated with BIM, the concentration of phospho-ERK1/2 or
bcl-2
in the BIM + 12-HETE-treated group was significantly (
P
< 0.05) lower than in that treated with 12-HETE only, and the concentration in the BIM + baicalein-treated group was significantly (
P
< 0.05) higher than in that treated with baicalein only. On the basis of these data we conclude that, via its metabolite 12-HETE, 12-LOX abolishes proliferation of AGS cells and protect cells from apoptosis by activating the ERK1/2 pathway and, eventually, enhances expression of
bcl-2
. Because PKC is also involved in the activation of ERK1/2 induced by 12-LOX, 12-LOX inhibitors would be potentially powerful anticancer agents for prevention and cure of human gastric cancer.
A novel Mn4+ activated Ca2LaSbO6 (CLS) far-red phosphor was synthesized by high temperature solid state reaction. The samples were characterized by X-ray diffraction (XRD), field emission scanning ...electron microscopy (FE-SEM), photoluminescence spectra, ultraviolet-visible spectra, luminescence decay times, emission-temperature relationship and internal quantum efficiency (IQE). It is found that CLS:Mn4+ phosphor has a strong broad excitation band in the range of 200–550 nm. The samples can be excited by ultraviolet and blue light. There is a wide emission band centered at 685 nm between 600 nm and 760 nm. The optimum doping concentration of Mn4+ is approximately 0.5 mol%. In addition, all the CIE chromaticity coordinates of CLS:0.005Mn4+ located at far-red region. The concentration quenching mechanism is the dipole-dipole interaction of Mn4+ activator. Importantly, the CLS:0.005 Mn4+ sample has an IQE of up to 52.2%. Finally, a 365 nm ultraviolet light emitting diode (LED) chip combined with 0.5 mol% Mn4+ far-red phosphor was used to fabricate the LED device. All the results indicated that CLS:Mn4+ phosphors have potential applications in indoor plant cultivation.
The effect of smelting processes on mechanical properties and microstructure of reduced activation ferritic steels was studied.Creep properties and impact toughness of reduced activation ferritic ...steels were obviously improved by vacuum induction melting followed by consumable electrode remelting process in comparison with the conventional vacuum induction melting process.The difference of impact toughness and creep properties between both steels mainly depended on the aspect ratio and mean size of precipitates.Decreasing the aspect ratio of carbides makes development of a shear band more difficult , which could increase impact energy and creep resistance.
To explore the clinical efficacy and prognosis of the patients with intermediate risk gastric stromal tumor (GST).
The data of intermediate risk GST patients confirmed by pathology and ...immunohistochemistry at Center of Gastrointestinal Surgery, West China Hospital, Sichuan University, between January 2007 and July 2011 were collected and retrospectively analyzed. And univariate and multivariate analyses were performed to assess the efficacy of imatinib mesylate (IM) according to recurrence-free survival (RFS).
A total of 46 intermediate risk GST patients were enrolled with a median follow-up period of 31 (9-64) months. Among them, 20 patients received IM treatment and 1 had hepatic metastasis at 16 months after withdrawal. In contrast, 5 of 26 patients refusing IM treatment recurred or had a distant metastasis. The 1-, 2-, 3-year recurrence-free survival in IM treatment group and no IM treatment group were: 20/20 vs 25/26, 14/14 vs 17/20 and 6/7 vs 10/14, respectively. Cox proportion hazards regression: hazar
Better pregnancy outcomes can be obtained by human mature oocyte vitrification, but many problems remain to be resolved in human mature oocyte vitrification. Since mature oocyte development possesses ...its own maturity cycle, there should be the optimal timing for mature oocyte vitrification. The purpose of this study was to observe the effects of frozen timing on the spindle density, the angle between the polar body and spindle, and embryo development of intracytoplasmic sperm injection (ICSI) in vitrified mouse mature oocytes and explore its possible mechanism. Mouse oocytes were randomly divided into three groups according to different frozen timing including Groups A, B, and C in which oocytes were vitrified within 2 h after ovum pick‐up, and 3–4 and 5–6 h after ovum pick‐up, respectively. Spindle‐related parameters were measured, ICSI was performed. The spindle occurrence rate of vitrified‐thawed oocytes was 98.4% in Group A, 82.3% in Group B, and 75.8% in Group C, without statistical differences between pre‐vitrification and post‐thawing and among the three groups (P > 0.05). The angles between the polar body and spindle were larger after thawing than before vitrification (P < 0.01). The spindle retardance values were lower after thawing than before vitrification in Groups B and C (P < 0.05), but higher in Group A (P < 0.05). The spindle retardance values before vitrification were higher in Group B than in Groups A and C (P < 0.05), but the spindle retardance value, oocyte survival and two‐cell rate after thawing were higher in Group A than in Groups B and C (P < 0.05). There were no statistical differences in ICSI fertility rate between the three groups (P > 0.05). The damage on the spindle is the slightest and embryo quality is the highest in the mouse oocytes vitrified within 2 h after ovum pick‐up. The spindle retardance value is more valuable than the spindle occurrence rate in the evaluation of vitrified‐thawed oocyte quality, and is positively correlated with embryo quality.
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