The current automatic scallion-transplanting machine is a complicated mechanism composed of two linkage mechanisms and two band carriers. It delivers seedlings inefficiently because of the movement ...limitations of the linkage mechanism. This paper proposes a new high-order non-circular gear train for an automatic scallion-seedling feeding mechanism. The proposed gear train has an asymmetrical transmission ratio; i.e., its transmission ratio varies. This allows the mechanism’s execution component to move in a long displacement and rotate in a large rotation angle. The long displacement enables the execution component to reach the designed working position, and the large rotation angle allows it to feed a scallion in the required pose. A mathematical model for calculating the asymmetrical transmission ratio was established according to the closure requirements and the full-cycle motion of the driven gear pitch curve. Then, the parameter-design model of the new seedling-feeding mechanism was established, based on precise pose points and trajectory-shape control points. Moreover, an aided-design program was developed to obtain the parameter-solution domain of the scallion-seedling feeding mechanism. The mechanism parameters, which met the seedling-feeding function, were optimized to determine the transmission ratio, using a program and a kinematic simulation. Finally, a prototype of the mechanism was produced, and a seedling-feeding experiment was carried out. One-thousand seedlings were tested at a rate of 100 seedlings per minute, and the statistical success rate was 93.4%. Thus, the automatic scallion-seedling feeding mechanism significantly improves the efficiency of automatically transplanting scallions.
Among all kinds of transplanting mechanisms which are the important parts of transplanters, the planetary gear train transplanting mechanism is widely used for its excellent transmission performance, ...but it is difficult to design the gear pitch curves and structural parameters according to the trajectory and pose requirements of transplanting. The current design method of the non-circular planetary gear train transplanting mechanism cannot ensure the precise position and posture in the crucial points of transplanting trajectory, nor take both the anticipated trajectory and the smoothness of the gear pitch curves into account, it will only get one solution which is available for mechanism design. In order to solve those problems, the non-circular planetary gear train transplanting mechanism is analyzed as a combination of the bar-group and gear train system. According to the transplanting requirements, three points with precise position and posture which called pose points in the trajectory are given to obtain the solution domain of the parameters of bar-group, and then trajectory shape control points are inlet to design the desired shape of the transplanting trajectory and obtain the transmission ratio curve which can be used to get the gear pitch curve. The most suitable parameters of bar-group in solution domain are selected based on the smoothness of the gear pitch curves. This article establishes three-dimensional model of the mechanism and utilizes the ADAMS to carry out a motion simulation; all simulation results are consistent with theoretical design results, which confirm that the design method based on prescribed pose points and trajectory control points is adequate for the transplanting mechanism. With the proposed method, the selectivity of the mechanism’s parameters solution is increased, and transmission performance benefits from the integrated design of trajectory control and the smoothness of the pitch curves.
The current automatic scallion-transplanting machine is a complicated mechanism composed of two linkage mecha-nisms and two band carriers. It delivers seedlings inefficiently because of the movement ...limitations of the linkage mechanism. This paper proposes a new high-order non-circular gear train for an automatic scallion-seedling feed-ing mechanism. The proposed gear train has an asymmetrical transmission ratio; i.e., its transmission ratio varies. This allows the mechanism's execution component to move in a long displacement and rotate in a large rotation angle.The long displacement enables the execution component to reach the designed working position, and the large rotation angle allows it to feed a scallion in the required pose. A mathematical model for calculating the asymmetrical transmission ratio was established according to the closure requirements and the full-cycle motion of the driven gear pitch curve. Then, the parameter-design model of the new seedling-feeding mechanism was established, based on precise pose points and trajectory-shape control points. Moreover, an aided-design program was developed to obtain the parameter-solution domain of the scallion-seedling feeding mechanism. The mechanism parameters, which met the seedling-feeding function, were optimized to determine the transmission ratio, using a program and a kinematic simulation. Finally, a prototype of the mechanism was produced, and a seedling-feeding experiment was carried out. One-thousand seedlings were tested at a rate of 100 seedlings per minute, and the statistical success rate was 93.4％. Thus, the automatic scallion-seedling feeding mechanism significantly improves the efficiency of automatically trans-planting scallions.
Abstract
Male breast cancer (MBC) is a rare but aggressive malignancy with cellular and immunological characteristics that remain unclear. Here, we perform transcriptomic analysis for 111,038 single ...cells from tumor tissues of six MBC and thirteen female breast cancer (FBC) patients. We find that that MBC has significantly lower infiltration of T cells relative to FBC. Metastasis-related programs are more active in cancer cells from MBC. The activated fatty acid metabolism involved with
FASN
is related to cancer cell metastasis and low immune infiltration of MBC. T cells in MBC show activation of p38 MAPK and lipid oxidation pathways, indicating a dysfunctional state. In contrast, T cells in FBC exhibit higher expression of cytotoxic markers and immune activation pathways mediated by immune-modulatory cytokines. Moreover, we identify the inhibitory interactions between cancer cells and T cells in MBC. Our study provides important information for understanding the tumor immunology and metabolism of MBC.
Systemic chemotherapy with multiple drug regimens is the main therapy option for advanced gastric cancer (GC) patients. However, many patients develop relapse soon. Here, we evaluated the therapeutic ...potential of targeting interleukin-8 (IL8) to overcome resistance to chemotherapy in advanced GC. RNA sequencing revealed crucial molecular changes after chemotherapy resistance, in which the expression of IL8 was significantly activated with the increase in drug resistance. Subsequently, the clinical significance of IL8 expression was determined in GC population specimens. IL8-targeted by RNA interference or reparixin reversed chemotherapy resistance with limited toxicity in vivo and vitro experiments. Sequential treatment with first-line, second-line chemotherapy and reparixin inhibited GC growth, reduced toxicity and prolonged survival. Collectively, our study provides a therapeutic strategy that targeting IL8 as a sequential therapy after chemotherapy resistance in advanced GC.
Introduction:
Urban spatial planning is critical for the development of sustainable and livable cities. However, traditional planning methods often face challenges in handling complex planning ...scenarios and large-scale data.
Methods:
This paper introduces UrbanGenoGAN, a novel algorithm that integrates generative adversarial networks (GANs), genetic optimization algorithms (GOAs), and geographic information system (GIS) to address these challenges. Leveraging the generative power of GANs, the optimization capabilities of genetic algorithms, and the spatial analysis capabilities of GIS, UrbanGenoGAN is designed to generate optimized urban plans that cater to various urban planning challenges. Our methodology details the algorithm’s design and integration of its components, data collection and preprocessing, and the training and implementation processes.
Results:
Through rigorous evaluation metrics, comparative analysis with existing methodologies, and case studies, the proposed algorithm demonstrates significant improvement in urban planning outcomes. The research also explores the technical and practical considerations for implementing UrbanGenoGAN, including scalability, computational efficiency, data privacy, and ethical considerations.
Discussion:
The findings suggest that the integration of advanced machine learning and optimization techniques with spatial analysis offers a promising approach to enhancing decision-making in urban spatial planning. This work contributes to the growing field of AI applications in urban planning and paves the way for more efficient and sustainable urban development.
Previous studies have revealed an increased risk of second primary malignancies (SPMs) after colorectal cancer (CRC); however, no previous investigation has quantified differences in the risk of SPMs ...based on the histological subtypes of first primary CRC.
Patients diagnosed with first primary CRC between 2000 and 2011 were identified from the Surveillance, Epidemiology, and End Results cancer registries. The patients were divided into three cohorts: classical adenocarcinoma (CA), mucinous adenocarcinoma (MA), and signet-ring cell carcinoma (SRCC). Standardized incidence ratios were calculated to assess the risk of SPMs among the patients.
Overall risk of SPMs was significantly higher among patients with three histological subtypes of CRC than in the general population. The risk of esophagus cancer was significantly increased in SRCC. The risk of small intestine, colon and rectum, and corpus uteri cancers was high in three histological subtypes, with the highest risk observed in SRCC, followed by MA. Increased risks of second stomach, uterus, urinary bladder, kidney, and thyroid cancers were only observed in CA patients, while increased risk of second renal pelvis cancer was limited to MA patients. Furthermore, the high overall risk of SPMs in CA patients persisted regardless of clinicopathological factors. After surgery combined with chemotherapy treatment, CA patients were more prone to developing second small intestine, colon and rectum cancers than those treated with surgery only. A lower second prostate cancer risk was observed in rectal CA patients treated with surgery combined with radiotherapy than in patients treated with surgery only.
The present study revealed that the risk of developing SPMs after CRC varied based on the histological subtypes of the first primary CRC. Although the mechanisms underlying the observed patterns of SPM risk remain unknown, the study provided insights into future cancer surveillance based on the histological subtypes of CRC.
Microtubules are a favorable target for development of anticancer agents. In this study, the anti-proliferative activities of plinabulin and six diketopiperazine derivatives were evaluated against ...human lung cancer cell line NCI-H460 and human pancreatic cancer cell line BxPC-3. The inhibition activities on these microtubules were assessed by tubulin polymerization and immunofluorescence assays. To gain insight into the interaction mechanism of the derivatives and tubulin, a molecular dynamics simulation was performed. We discovered that the diketopiperazine derivatives could prevent tubulin assembly through conformational changes. Molecular Mechanics/Poisson-Boltzmann Surface Area (MM-PBSA) calculations showed that the trend of the binding free energies of these inhibitors was in agreement with the trend of their biological activities. Introducing hydrophobic groups into the A-ring was favorable for binding. Energy decomposition indicated that van der Waals interaction played an essential role in the binding affinity of tubulin polymerization inhibitors. In addition, the key residues responsible for inhibitor binding were identified. In summary, this study provided valuable information for development of novel tubulin polymerization inhibitors as anticancer agents.
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•Co-crystal structure guided rational design.•Novel compounds with higher activity against human BxPC-3 cell line.•Interaction explained by molecular docking studies.
Based on the ...co-crystal structures of tubulin with plinabulin and Compound 1 (a derivative of plinabulin), a total of 18 novel plinabulin derivatives were designed and synthesized. Their biological activities were evaluated against human pancreatic cancer BxPC-3 cell lines. Two novel Compounds 13d and 13e exhibited potent activities with IC50 at 1.56 and 1.72 nM, respectively. The tubulin polymerization assay indicated that these derivatives could inhibit microtubule polymerization. Furthermore, the interaction between tubulin and these compounds were elucidated by molecular docking. The binding modes of Compounds 13d and 13e were similar to the co-crystal structure of Compound 1. H-π interaction was observed between the aromatic hydrogen of thiophene moiety with Phe20, which could enhance their binding affinities.
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