The main difficulty of ovarian tissue autograft (OTA) is to preserve as many follicles as possible because the ovarian tissue undergoes warm ischemia during grafting until revascularisation, ...resulting in significant follicular loss. We describe a two-stage grafting technique to stimulate new vascularisation in order to enhance the revascularization process to reduce the ischemic injuries. Furthermore we performed ovarian patchwork in the laboratory and then grafting with robotic laparoscopy to facilitate surgery and increase precision. This technique is used in the DATOR study with promising results, such as a 40% delivery rate.
Tyrosine kinase inhibitors are now major actors for the treatment of non-small-cell metastatic lung cancers where ROS 1 gene rearrangement is present. Because of the rapid development of these new ...therapies, developing information about their monitoring and knowledge about their potential toxicities is essential. We describe the case of a patient who was treated with ceritinib as a third line approach for a metastatic lung adenocarcinoma with ROS1 rearrangement. After two months, the patient developed acute respiratory distress with pericarditis and pleurisy. A hypersensitivity reaction was suggested and supported by favorable clinical and radiological outcomes within three days following ceritinib discontinuation and systemic corticosteroid introduction. Pleural effusion, pericarditis and diffuse pulmonary infiltration associated to ceritinib have not often been described previously. Despite few data of pulmonary toxicity related to ceritinib, the current observation highlights the need for caution and regular monitoring when using these inhibitors.
Acquired hemophilia A (AHA) is a rare disease, defined by the production of anti-factor VIII antibodies causing disordered hemostasis. It is idiopathic in 50% of cases, but sometimes associated with ...solid tumors. We report a case where AHA led to the diagnosis of lung cancer.
An 82-year-old man with spontaneous hematomas on his trunk and extremities, and isolated prolongation of activated partial thromboplastin time was admitted to the emergency room. A severely reduced factor VIII level and a high factor VIII inhibitor title confirmed the diagnosis of AHA. Thoracic computed tomography scan found a suspect lung nodule and biopsy was consistent with a primary lung adenocarcinoma. The patient received recombinant factor VIII, immunosuppressive therapies, and finally lung stereotactic radiotherapy. Thirty months after diagnosis, the patient is in complete remission both from AHA and from his lung cancer.
Acquired hemophilia A is a rare but potentially severe disease, which may be idiopathic or linked to a solid tumor. The severity of AHA depends on both the volume of hemorrhage and the presence of associated diseases.
A novel passive microfluidic silicon mixer has been designed, optimized and fabricated. The architecture of the mixer consists of a simple "T" junction, made up by a 20 microm wide by 82 microm deep ...channel, followed by three repeats of an alcove, each with a triangular obstruction, arranged in a zigzag fashion. Numerical simulations were employed to optimize the geometry, particularly the dimensions of the alcoves, the relative orientation and the spacing between them, and the degree of intrusion associated with them. The simulation results demonstrate that chaotic flow due to recirculation within the alcoves results in transverse velocity that promotes effective fluid mixing. The microfluidic mixer with the simulation-optimized geometry was fabricated with photolithographic techniques and characterized by optical imaging, fluorescence, and Raman microscope spectroscopy. At a sample flow rate of 20 microL/s, the mixer exhibits a short mixing deadtime of approximately 22 micros and a high mixing efficiency under both low and high viscosity conditions. The alcove-based microfluidic silicon mixer offers unique advantages for its short deadtime and slow sample consumption rate. In addition, it provides a valuable component for laboratory-on-a-chip applications for its ease of development into multiple networks for massively parallel analytical processes.
An in-line coagulation (without settling)/UF process has been studied to improve membrane performance and water quality for surface water treatment. Using coagulation before UF increases permeate ...quality; the extent of dissolved organic matter removal is controlled by the coagulation step. Efficient coagulation conditions for a coagulation/settling process can be applied for the in-line coagulation/UF process and membrane fouling is reduced. Floc cake resistance is lower than resistance due to the unsettled floc and the uncoagulated organics. For an inside-out hollow-fiber system, the impact of coagulation dose depends on filtration mode: for instance, in cross-flow with a feed-and-bleed configuration, a reduction of coagulant dose induces an increase of the mass transfer resistance even in quasi-stable hydrodynamic operating conditions.
Describes a new approach based on the time-frequency representation of transient nonlinear chirp signals for modeling the aortic (A2) and the pulmonary (P2) components of the second heart sound (S2). ...It is demonstrated that each component is a narrow-band signal with decreasing instantaneous frequency defined by its instantaneous amplitude and its instantaneous phase. Each component Is also a polynomial phase signal, the instantaneous phase of which can be accurately represented by a polynomial having an order of thirty. A dechirping approach is used to obtain the instantaneous amplitude of each component while reducing the effect of the background noise. The analysis-synthesis procedure is applied to 32 isolated A2 and 32 isolated P2 components recorded in four pigs with pulmonary hypertension. The mean /spl plusmn/ standard deviation of the normalized root-mean-squared error (NRMSE) and the correlation coefficient (/spl rho/) between the original and the synthesized signal components were: NRMSE=2.1/spl plusmn/0.3% and /spl rho/=0.97/spl plusmn/0.02 for A2 and NRMSE=2.52/spl plusmn/0.5% and /spl rho/=0.96/spl plusmn/0.02 for P2. These results confirm that each component can be modeled as mono-component nonlinear chirp signals of short duration with energy distributions concentrated along its decreasing instantaneous frequency.
The aim of this study was to encapsulate ethylhexyl methoxycinnamate (EMC), a commonly used UVB filter, in a solid lipid matrix in order to obtain microparticles and then nanoparticles to reduce its ...photo-instability under UV light exposure. Glyceryl behenate, rice bran wax and ozokerite were investigated for encapsulating EMC. The suspensions of nanoparticles contained 70% encapsulated EMC (relative to the lipid mass). The absorbance level at 310 nm of suspensions containing nanoparticles was more than twice that of those containing microparticles. So, decreasing the size of particles improved the efficiency of light protection, regardless of the lipid material used. Moreover, free EMC presented a 30% loss of its efficiency after 2 h of irradiation, whereas the three NLC formulations showed a loss of absorbency between 10% and 21%. The in vitro cutaneous penetration test did not show a higher potential penetration for EMC contained in nanosuspensions compared to free EMC.
To determine the influence of topical vancomycin prophylaxis on the incidence of bacterial endophthalmitis in eyes with a Boston Keratoprosthesis (KPro).
A retrospective chart review was performed ...for 255 eyes of 231 patients who received a KPro between March 1990 and December 2006. Preoperative diagnoses were burn, ocular cicatricial pemphigoid (OCP), Stevens-Johnson Syndrome (SJS), and graft failure/other. Patients used topical antibiotic prophylaxis for the duration of the KPro: polymyxin-trimethoprim or a quinolone in the 1990s, or a quinolone with or without vancomycin beginning in late 1999. For each KPro eye, the follow-up interval was divided into months on or off vancomycin (vancomycin versus no-vancomycin group). The incidence of endophthalmitis was calculated with Kaplan-Meier survival curves.
The 255 eyes were followed for 673.6 patient-years (mean, 2.64 years; range, 1 week to 13 years). There were 18 cases of bacterial endophthalmitis; 17 occurred at least 6 weeks postoperatively (range, 1.5 to 46 months). Gram-positive cocci caused over 80% of cases. Only 1 case, due to an atypical mycobacterium, occurred in a patient using vancomycin.The incidence of bacterial endophthalmitis was lower in the vancomycin group than in the no-vancomycin group: 0.35% versus 4.13% per patient-year (P = 0.001). It was also lower in SJS eyes using vancomycin versus no vancomycin: 1.76% versus 18.39% per patient-year (P = 0.009). In eyes with preoperative diagnoses of burn, OCP, or graft failure/other, the incidence in the vancomycin group was zero.
Topical vancomycin plus a quinolone is effective in preventing bacterial endophthalmitis in KPro eyes.
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