Endometriosis is a chronic, estrogen-dependent gynecological condition affecting approximately 10% of reproductive age women. The most widely accepted theory of its etiology includes retrograde ...menstruation. Recent reports suggest the uterus is not sterile. Thus, the refluxed menstrual effluent may carry bacteria, and contribute to inflammation, the establishment and growth of endometriotic lesions. Here, we compared and contrasted uterine bacteria (endometrial microbiota) in people with surgically confirmed presence (N = 12) or absence of endometriosis (N = 9) using next-generation 16S rRNA gene sequencing. We obtained an average of > 9000 sequence reads per endometrial biopsy, and found the endometrial microbiota of people with endometriosis was more diverse (greater Shannon Diversity Index and proportion of 'Other' taxa) than symptomatic controls (with pelvic pain, surgically confirmed absence of endometriosis; diagnosed with other benign gynecological conditions). The relative abundance of bacterial taxa enriched in the endometrial microbiota of people with endometriosis belonged to the Actinobacteria phylum (Gram-positive), Oxalobacteraceae (Gram-negative) and Streptococcaceae (Gram-positive) families, and Tepidimonas (Gram-negative) genus, while those enriched in the symptomatic controls belonged to the Burkholderiaceae (Gram-negative) family, and Ralstonia (Gram-negative) genus. Taken together, results suggest the endometrial microbiota is perturbed in people with endometriosis.
The fourth catalog of active galactic nuclei (AGNs) detected by the Fermi Gamma-ray Space Telescope Large Area Telescope (4LAC) between 2008 August 4 and 2016 August 2 contains objects located at ...high Galactic latitudes ( ). It includes 85% more sources than the previous 3LAC catalog based on 4 yr of data. AGNs represent at least 79% of the high-latitude sources in the fourth Fermi-Large Area Telescope Source Catalog (4FGL), which covers the energy range from 50 MeV to 1 TeV. In addition, gamma-ray AGNs are found at low Galactic latitudes. Most of the 4LAC AGNs are blazars (98%), while the remainder are other types of AGNs. The blazar population consists of 24% Flat Spectrum Radio Quasars (FSRQs), 38% BL Lac-type objects, and 38% blazar candidates of unknown types (BCUs). On average, FSRQs display softer spectra and stronger variability in the gamma-ray band than BL Lacs do, confirming previous findings. All AGNs detected by ground-based atmospheric Cerenkov telescopes are also found in the 4LAC.
Abstract
As a follow-up to the optical spectroscopic campaign aimed at achieving completeness in the Third Catalog of Hard Fermi-LAT Sources (3FHL), we present here the results of a sample of 28 ...blazars of an uncertain type observed using the 4 m telescope at Cerro Tololo Inter-American Observatory in Chile. Out of these 28 sources, we find that 25 are BL Lacertae objects (BL Lacs) and 3 are flat-spectrum radio quasars (FSRQs). We measure redshifts or lower limits for 16 of these blazars, and it is observed that the 12 remaining blazars have featureless optical spectra. These results are part of a more extended optical spectroscopy follow-up campaign for 3FHL blazars, where, until now, 51 blazars of an uncertain type have been classified into BL Lac and FSRQ categories. Furthermore, this campaign has resulted in redshift measurements and lower limits for 15 of these sources. Our results contribute toward attaining a complete sample of blazars above 10 GeV, which then will be crucial in extending our knowledge on blazar emission mechanisms and the extragalactic background light.
We present a likelihood profile stacking technique based on the Fermi-Large Area Telescope (LAT) data to explore the γ-ray characteristics of Fermi-LAT undetected astrophysical populations. The ...pipeline is applied to a sample of γ-ray unresolved extreme blazars, i.e., sources with the highest synchrotron peak frequencies ( ), and we report a cumulative γ-ray detection with more than 32 confidence for 2 degrees of freedom. Comparing the generated stacked γ-ray spectrum with the sensitivity limits of the upcoming Cherenkov Telescope Array (CTA), we find that the Fermi-LAT undetected population of such extreme blazars, on average, may remain well below the CTA detection threshold due to their faintness and extragalactic background light (EBL) absorption. However, γ-ray detected blazars belonging to the same class are promising candidates for CTA observations. The EBL-corrected stacked spectra of these sources do not show any softening up to 1 TeV. This finding suggests the inverse Compton peak of extreme blazars lies above 1 TeV, thus indicating a hard intrinsic TeV spectrum. Our analysis also predicts that at 100 GeV, at least ∼10% of the diffuse extragalactic γ-ray background originates from the γ-ray undetected extreme blazars. These results highlight the effectiveness of the developed stacking technique to explore the uncharted territory of γ-ray undetected astrophysical objects.
Thallium (Tl) and its compounds are non-essential and highly toxic for living organisms, even at low concentrations. In this paper, we analyzed the presence and geochemical distribution of Tl in ...different mining-metallurgical and sediment samples collected from several mining zones of Mexico. A modified BCR sequential extraction procedure was also applied to the samples to investigate the geochemical behavior and potential environmental risk of Tl according to types of ore deposit and mineral processing method applied. Results revealed the presence of Tl in the majority of the mining-metallurgical samples, with labile concentrations reaching up to values of 184.4 mg kg−1, well above the environmental standards. A comparison of Tl partitioning in different samples showed that Tl was usually found associated with labile fractions instead of entrapped in the environmentally-passive residual fraction. Specifically, high levels of Tl were extracted from the exchangeable/acid-extractable and poorly-crystalline reducible fractions, suggesting its association with both soluble and amorphous Fe-Mn oxyhydroxides, respectively. Besides, Tl was also frequently found associated with the crystalline reducible fraction, presumably bonded to manganese oxides and jarosite-like minerals. Lastly, little amounts of Tl were extracted from the oxidizable fraction. Considering the fractionation of Tl in these mining-metallurgical samples, they may pose a significant environmental hazard. This study provides useful insights into the potential sources of Tl pollution in Mexico and emphasizes the need for further research to determine the extent of its impact and to develop effective remediation protocols to protect the environment from Tl toxicity.
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•Mining-metallurgical residues are potential Tl sources in mining districts of Mexico.•A relationship between wastes and Tl-impacted streambed sediments was established.•A four-step sequential extraction procedure was carried out to assess Tl distribution.•Very high concentrations of weakly-bound Tl were associated within the labile fraction.•Weathering of residues triggered Tl dispersion by solubilization and colloidal transport.
Large concentrations of labile Tl were found in hazardous mining-metallurgical residues, affecting sediments and ephemeral streams that eventually feed local aquifers.
Growing evidence demonstrates a strong correlation between obesity and an increased risk of breast cancer, although the mechanisms involved have not been completely elucidated. Some reports have ...described a crosstalk between adipocytes, cancer cells, and immune cells within the tumor microenvironment, however, it is currently unknown whether obesity can promote tumor growth by inducing systemic alterations of the immune cell homeostasis in peripheral lymphoid organs and adipose tissue. Here, we used the E0771 breast cancer cell line in a mouse model of diet-induced obesity to analyze the immune subpopulations present in the tumors, visceral adipose tissue (VAT), and spleen of lean and obese mice. Our results showed a significant reduction in the frequency of infiltrating CD8+ T cells and a decreased M1/M2 macrophage ratio, indicative of the compromised anti-tumoral immune response reported in obesity. Despite not finding differences in the percentage or numbers of intratumoral Tregs, phenotypic analysis showed that they were enriched in CD39+, PD-1+ and CCR8+ cells, compared to the draining lymph nodes, confirming the highly immunosuppressive profile of infiltrating Tregs reported in established tumors. Analysis of peripheral T lymphocytes showed that tumor development in obese mice was associated to a significant increase in the percentage of peripheral Tregs, which supports the systemic immunosuppressive effect caused by the tumor. Interestingly, evaluation of immune subpopulations in the VAT showed that the characteristic increase in the M1/M2 macrophage ratio reported in obesity, was completely reversed in tumor-bearing mice, resembling the M2-polarized profile found in the microenvironment of the growing tumor. Importantly, VAT Tregs, which are commonly decreased in obese mice, were significantly increased in the presence of breast tumors and displayed significantly higher levels of Foxp3, indicating a regulatory feedback mechanism triggered by tumor growth. Altogether, our results identify a complex reciprocal relationship between adipocytes, immune cells, and the tumor, which may modulate the immune macroenvironment that promotes breast cancer development in obesity.
The recent spatial and temporal coincidence of the blazar TXS 0506+056 with the IceCube-detected neutrino event IC-170922A has opened up a realm of multimessenger astronomy with blazar jets as a ...plausible site of cosmic-ray acceleration. After TXS 0506+056, a second blazar, BZB J0955+3551, was recently found to be spatially coincident with the IceCube-detected neutrino event IC-200107A and undergoing its brightest X-ray flare measured so far. Here we present the results of our multifrequency campaign to study this peculiar event that includes observations with the NuSTAR, Swift, Neutron star Interior Composition Explorer (NICER), and 10.4 m Gran Telescopio Canarias (GTC). The optical spectroscopic observation from GTC secured its redshift as and the central black hole mass as . Both NuSTAR and NICER data reveal a rapid flux variability, albeit at low significance ( ). We explore the origin of the target photon field needed for the photopion production using analytical calculations and considering the observed optical-to-X-ray flux level. We conclude that seed photons may originate from outside the jet, similar to that reported for TXS 0506+056, although a scenario invoking a comoving target photon field (e.g., electron synchrotron) cannot be ruled out. The electromagnetic output from the neutrino-producing photohadronic processes are likely to make only a subdominant contribution to the observed spectral energy distribution, suggesting that the X-ray flaring event may not be directly connected with IC-200107A.
• The evolution of plant defenses has traditionally been studied at single plant ontogenetic stages, overlooking the fact that natural selection acts continuously on organisms along their ...development, and that the adaptive value of phenotypes can change along ontogeny.
• We exposed 20 replicated genotypes of Turnera velutina to field conditions to evaluate whether the targets of natural selection on different defenses and their adaptative value change across plant development.
• We found that low chemical defense was favored in seedlings, which seems to be explained by the assimilation efficiency and the ability of the specialist herbivore to sequester cyanogenic glycosides. Whereas trichome density was unfavored in juvenile plants, it increased relative plant fitness in reproductive plants. At this stage we also found a positive correlative gradient between cyanogenic potential and sugar content in extrafloral nectar.
• We visualize this complex multi-trait combination as an ontogenetic defensive strategy. The inclusion of whole-plant ontogeny as a key source of variation in plant defense revealed that the targets and intensity of selection change along the development of plants, indicating that the influence of natural selection cannot be inferred without the assessment of ontogenetic strategies in the expression of multiple defenses.
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