Many fish encounter hypoxia in their native environment, but the role of mitochondrial physiology in hypoxia acclimation and hypoxia tolerance is poorly understood. We investigated the effects of ...hypoxia acclimation on mitochondrial respiration, O2kinetics, emission of reactive oxygen species (ROS), and antioxidant capacity in the estuarine killifish ( ITALIC! Fundulus heteroclitus). Killifish were acclimated to normoxia, constant hypoxia (5 kPa O2) or intermittent diel cycles of nocturnal hypoxia (12 h:12 h normoxia:hypoxia) for 28-33 days and mitochondria were isolated from liver. Neither pattern of hypoxia acclimation affected the respiratory capacities for oxidative phosphorylation or electron transport, leak respiration, coupling control or phosphorylation efficiency. Hypoxia acclimation also had no effect on mitochondrial O2kinetics, but ITALIC! P50(the O2tension at which hypoxia inhibits respiration by 50%) was lower in the leak state than during maximal respiration, and killifish mitochondria endured anoxia-reoxygenation without any impact on mitochondrial respiration. However, both patterns of hypoxia acclimation reduced the rate of ROS emission from mitochondria when compared at a common O2tension. Hypoxia acclimation also increased the levels of protein carbonyls and the activities of superoxide dismutase and catalase in liver tissue (the latter only occurred in constant hypoxia). Our results suggest that hypoxia acclimation is associated with changes in mitochondrial physiology that decrease ROS production and may help improve hypoxia tolerance.
Although effluent from municipal wastewater treatment plants (WWTPs) is a major stressor in receiving environments, relatively few studies have addressed how its discharge affects natural fish ...communities. Here, we assessed fish community composition over three years along a gradient of effluent exposure from two distinct WWTPs within an International Joint Commission Area of Concern on the Great Lakes (Hamilton Harbour, Canada). We found that fish communities changed with distance from both WWTPs, and were highly dissimilar between sites that were closest to and furthest from the wastewater outfall. Despite differences in the size and treatment technology of the WWTPs and receiving habitats downstream, we found that the sites nearest the outfalls had the highest fish abundances and contained a common set of signature fish species (i.e., round goby Neogobius melanostomus, green sunfish Lepomis cyanellus). Non-native and stress tolerant species were also more abundant near one of the studied WWTPs when compared to the reference site, and the number of young-of-the-year fish collected did not vary along the effluent exposure gradients. Overall, we show that fish are attracted to wastewater outfalls raising the possibility that these sites may act as an ecological trap.
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•Fish communities sampled over multiple years along two WWTP effluent gradients.•Fish communities diverged along both effluent gradients.•Higher abundance of fish near both WWTP outfalls.•“Signature” species collected a both WWTP outfalls.•WWTP effluents affected water quality downstream.
Mitochondrial dysfunction and oxidative stress have been suggested to be possible mechanisms underlying hybrid breakdown, as a result of mito-nuclear incompatibilities in respiratory complexes of the ...electron transport system. However, it remains unclear whether hybridization increases the production of reactive oxygen species (ROS) by mitochondria. We used high-resolution respirometry and fluorometry on isolated liver mitochondria to examine mitochondrial physiology and ROS emission in naturally occurring hybrids of pumpkinseed (Lepomis gibbosus) and bluegill (L. macrochirus). ROS emission was greater in hybrids than in both parent species when respiration was supported by complex I (but not complex II) substrates, and was associated with increases in lipid peroxidation. However, respiratory capacities for oxidative phosphorylation, phosphorylation efficiency, and O₂ kinetics in hybrids were intermediate between those in parental species. Flux control ratios of capacities for electron transport (measured in uncoupled mitochondria) relative to oxidative phosphorylation suggested that the limiting influence of the phosphorylation system is reduced in hybrids. This likely helped offset impairments in electron transport capacity and complex III activity, but contributed to augmenting ROS production. Therefore, hybridization can increase mitochondrial ROS production, in support of previous suggestions that mitochondrial dysfunction can induce oxidative stress and thus contribute to hybrid breakdown.
Effluent from wastewater treatment plants (WWTP) contains a complex mixture of contaminants and is a major worldwide source of aquatic pollution. We examined the effects of exposure to treated ...effluent from a municipal WWTP on the metabolic physiology of bluegill sunfish (Lepomis macrochirus). We studied fish that were wild-caught or experimentally caged (28 d) downstream of the WWTP, and compared them to fish that were caught or caged at clean reference sites. Survival was reduced in fish caged at the effluent-contaminated site compared to those caged at the reference site. Resting rates of O2 consumption (MO2) were higher in fish from the contaminated site, reflecting a metabolic cost of wastewater exposure. The increases in routine MO2 did not reduce aerobic scope (difference or quotient of maximal MO2 and resting MO2), suggesting that physiological compensations accompanied the metabolic costs of wastewater exposure. Fish exposed to wastewater also had larger hearts and livers. The activity of mitochondrial enzymes (cytochrome c oxidase, citrate synthase) per liver mass was unaltered across treatments, so the increased mass of this organ increased its cumulative oxidative capacity in the fish. Wastewater exposure also reduced glycogen content per liver mass. The effects of caging itself, based on comparisons between fish that were wild-caught or caged at clean sites, were generally subtle and not statistically significant. We conclude that exposure to wastewater effluent invokes a metabolic cost that leads to compensatory physiological adjustments that partially offset the detrimental metabolic impacts of exposure.
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•Exposure to treated effluent from a municipal wastewater treatment plant reduced survival in bluegill sunfish.•Exposure also invokes a significant metabolic cost that increased resting metabolic rate in survivors.•Surviving bluegill compensated for this metabolic cost and did not suffer a reduction in aerobic scope.•Exposed bluegill had larger hearts and livers, greater metabolic capacity in the liver, but reduced liver glycogen content.
Abstract
Wastewater from municipal, agricultural and industrial sources is a pervasive contaminant of aquatic environments worldwide. Most studies that have investigated the negative impacts of ...wastewater on organisms have taken place in a laboratory. Here, we tested whether fish behaviour is altered by exposure to environmentally relevant concentrations of wastewater effluent in the field. We caged bluegill sunfish (Lepomis macrochirus) for 28 days at two sites downstream (adjacent to and 870 m) from a wastewater treatment plant and at a reference site without wastewater inputs. We found that exposed fish had a dampened response to simulated predation compared to unexposed fish, suggesting that fish may be at greater risk of predation after exposure to wastewater effluent. Fish held at the different sites did not differ in activity and exploration. Our results suggest that predator avoidance may be impaired in fish exposed to wastewater effluent, which could have detrimental implications for aquatic communities.
•Three week sub-chronic field caging exposure to wastewater effluent.•Tested a non-model invasive fish species.•Reduced survival at highest wastewater exposure sites.•Few behavioural or physiological ...effects in survivors.
Treated effluents from wastewater treatment plants (WWTP) are a significant source of anthropogenic contaminants, such as pharmaceuticals, in the aquatic environment. Although our understanding of how wastewater effluent impacts fish reproduction is growing, we know very little about how effluent affects non-reproductive physiology and behaviours associated with fitness (such as aggression and activity). To better understand how fish cope with chronic exposure to wastewater effluent in the wild, we caged round goby (Neogobius melanostomus) for three weeks at different distances from a wastewater outflow. We evaluated the effects of this exposure on fish survival, behaviour, metabolism, and respiratory traits. Fish caged inside the WWTP and close to the outfall experienced higher mortality than fish from the reference site. Interestingly, those fish that survived the exposure performed similarly to fish caged at the reference site in tests of aggressive behaviour, startle-responses, and dispersal. Moreover, the fish near WWTP outflow displayed similar resting metabolism (O2 consumption rates), hypoxia tolerance, haemoglobin concentration, haematocrit, and blood-oxygen binding affinities as the fish from the more distant reference site. We discuss our findings in relation to exposure site water quality, concentrations of pharmaceutical and personal care product pollutants, and our test species tolerance.
Municipal wastewater treatment plants (WWTPs) are a significant source of anthropogenic pollutants and are a serious environmental stressor in Laurentian Great Lakes ecosystems. In this study, we ...examined whether three freshwater fish species (bluegill sunfish Lepomis macrochirus, green sunfish Lepomis cyanellus, and round goby Neogobius melanostomus) collected near two wastewater effluent outflows in Lake Ontario showed altered measures of somatic investment and thermal tolerance. Fish of all three species collected near the WWTPs were larger with 50–60% heavier body masses compared to those collected at reference sites. Green sunfish had higher body condition and increased haematocrit at wastewater-contaminated sites, and both round goby and bluegill sunfish had larger livers (controlling for body mass) at wastewater-contaminated sites. Thermal tolerance (critical thermal maximum, CTmax) differed between species (green sunfish > bluegill sunfish > round goby), but was similar in fish collected at wastewater-contaminated sites compared to cleaner reference sites. Wastewater-contaminated sites had poorer water quality, higher nutrient loadings, and higher concentrations of anthropogenic contaminants (measured via polar organic chemical integrative samplers, POCIS) than reference sites. Our results suggest that fish in the wild may have some capacity to cope with WWTP effluent and avoid any potential impairments in thermal tolerance. Our findings also suggest that treated wastewater is changing water quality locally in Great Lakes watersheds, and that many fish species may be able to access extra nutrients provided by such effluent outflows. However, if outflow areas become preferred foraging areas this will inadvertently increase exposure to anthropogenic stressors and pollutants.
Failure is hard‐wired into the scientific method and yet teaching students to productively engage with failure is not foundational in most biology curricula. To train successful scientists, it is ...imperative that we teach undergraduate science students to be less fearful of failure and to instead positively accept it as a productive part of the scientific process. In this article, we focus on student perceptions of the stigma of failure and their associated concerns to explore how failure could be better supported within and beyond a university context. Through a survey of first‐year biology students, we found that societal and familial pressures to succeed were the greatest contributing factors to students' fear of failure. In student suggestions on how to reduce the stigma of failure within and beyond the university context, the most common theme identified across both contexts was for increased discussion and open communication about experiences of failure. Importantly, student comments in this study bring attention to the role of factors beyond the classroom in shaping student experiences of failure within their biology courses.
To train successful scientists, we must teach undergraduate students to be less fearful of failure as it is a productive part of the scientific process. An investigation of student perceptions of failure found that societal and familial pressures were the greatest contributing factors to fear of failure. To decrease the stigma of failure, students suggest increased discussion of failure experiences.
De-icing road salt is a persistent emerging pollutant in temperate freshwater systems, where winter salting is necessary for road and pedestrian safety. Experts argue that road salts may increase ...salt-tolerant mosquito populations and, potentially, disease transmission in urban areas. Only adult females consume bloodmeals and may carry zoonotic diseases. While there are some species with naturally occurring male-biased sex ratios, it is unclear whether road salt differentially affects male and female mosquitoes to alter sex ratios. We hypothesized that road salts would masculinize emergence sex ratios and decrease female success because females may face higher exposure to stressors during their lengthy juvenile development compared to males. We measured mosquito emergence sex ratios of control (0 g/L added salt) and salt (4.5 g/L added salt) mesocosms in southern Ontario, Canada across the West Nile Virus season (May to October). We found female-biased sex ratios (i.e., <50% male frequency) in both 0 and 4.5 g/L. While mosquito abundance was significantly higher in 4.5 g/L compared to 0 g/L, road salt significantly increased the proportion of emerging males from 32.8% to 40.8% (Negative Binomial Model; Estimate ± SE = 0.283 ± 0.108; P = 0.009); mosquitoes shift their sex ratios from female-biased towards parity (50:50) in response to salt. Our study illustrates the need to evaluate sex-specific abundance in pollution-related mosquito population studies. By showing a shift toward more male mosquitoes emerging in high salinity compared to control treatments, our results suggest that road salts may have the potential to decrease female mosquito success and indirectly reduce disease transmission in cities.
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•Road salts run off into adjacent freshwater environments.•Urban mosquito populations display female-biased adult sex ratios.•Road salt (4.5 g/L) shifts mosquito sex ratios from 32.9% to 40.8% males.•4.5 g/L salt drives total adult mosquito abundance up by 64.5%.•Changes in sex ratios limit mosquito reproduction and disease spread.
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