The current research aimed to estimate the effect of dietary supplementation with glycinin isolated from soybeans on the growth performance, carcass traits, and selected blood metabolites of broiler ...chicks. A total of 200 1-wk-old broiler chicks were administered diets without glycinin (control treatment) or diets supplemented with 3 concentrations of soy glycinin (0.5, 1.0, or 1.5 g/kg of feed) for 6 wk. At the end of the feeding period, body weight was significantly higher in broiler chicks with glycinin supplementation (P < 0.05 or 0.01). The best values for body weight and body weight gain were recorded in the groups fed diets supplemented with 0.5 and 1.0 g glycinin/kg feed. Feed conversion was significantly (P < 0.05) improved in broilers in the glycinin-supplemented groups during the 1 to 6 and 3 to 6 wk growth periods. The highest value of breast yield was observed in broiler chicks supplemented with glycinin at a concentration of 1.0 g/kg of feed. Water-holding capacity increased with increasing concentrations of glycinin in the feed, up to 1.0%. Serum creatinine and urea concentrations decreased gradually (P < 0.01) as the concentration of glycinin in the feed increased. Broiler chicks receiving increasing concentrations of glycinin exhibited significantly (P < 0.01) lower levels of serum triglycerides, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol. All meat samples from broiler chicks supplemented with glycinin had significantly higher catalase activities. These data suggest that feeding broiler chicks diets supplemented with soy glycinin (0.5 to 1.5 g/kg of feed) can improve feed conversion, enhance body weight gain, and lower abdominal fat.
Introduction:
Purslane (
Portulaca oleracea
L.) seeds oil are a non-traditional alpha-linolenic acid source (ALA), which is an omega-3 fatty acid. This study aimed to evaluate the physicochemical and ...sensory properties of mango juice fortified with purslane seed oil (PSO) microcapsules.
Materials and Methods:
Gum Arabic (GA) and maltodextrin, as wall-materials, were used in the microencapsulation of PSO by spray drying technique. The spray-dried microcapsules were added to the mango juice (200 mL) at the levels of 0.5, 1 and 1.5 g, ALA. Physicochemical properties such as viscosity, total soluble solids (TSS), pH and titratable acidity were measured, as well as sensory evaluation, during 28 days' storage at 4.0 ±0.5°C.
Results:
Our study indicated that the microencapsulation of PSO by spray drying resulted in the best microencapsulation yield (85.17%) as well as the microencapsulation efficiency (77.40%). The pH and TSS of four juice samples ranged from 3.0 to 3.6 and from 18.8 to 19.1 Brix°, respectively. In addition to that, storage periods had no significant effect on them.
Conclusion:
According to the findings presented in this paper, it has been concluded that the nutritional value of mango juices was enhanced by the addition of microencapsulated PSO as a source of ω-3 fatty acids.
Hepatocellular carcinoma (HCC) is a highly prevalent form of liver cancer that necessitates accurate prediction models for early diagnosis and effective treatment. Machine learning algorithms have ...demonstrated promising results in various medical domains, including cancer prediction. In this study, we propose a comprehensive approach for HCC prediction by comparing the performance of different machine learning algorithms before and after applying feature reduction methods. We employ popular feature reduction techniques, such as weighting features, hidden features correlation, feature selection, and optimized selection, to extract a reduced feature subset that captures the most relevant information related to HCC. Subsequently, we apply multiple algorithms, including Naive Bayes, support vector machines (SVM), Neural Networks, Decision Tree, and K nearest neighbors (KNN), to both the original high-dimensional dataset and the reduced feature set. By comparing the predictive accuracy, precision, F Score, recall, and execution time of each algorithm, we assess the effectiveness of feature reduction in enhancing the performance of HCC prediction models. Our experimental results, obtained using a comprehensive dataset comprising clinical features of HCC patients, demonstrate that feature reduction significantly improves the performance of all examined algorithms. Notably, the reduced feature set consistently outperforms the original high-dimensional dataset in terms of prediction accuracy and execution time. After applying feature reduction techniques, the employed algorithms, namely decision trees, Naive Bayes, KNN, neural networks, and SVM achieved accuracies of 96%, 97.33%, 94.67%, 96%, and 96.00%, respectively.
Background
Aplastic anemia (AA) is a bone marrow disorder characterized by peripheral pancytopenia and marrow hypoplasia which can lead to life-threatening complications. Our objective was to study ...the telomerase genes (
TERT
and
TERC
) variants, explore their relationship to telomere shortening and
TERT
gene expression, and to identify variants in the
MPL
gene within Egyptian AA patients.
Methods
Forty AA patients and 40 sex- and age-matched healthy individuals as the control group were studied through sequencing of
TERT
,
TERC
, and
MPL
genes. Quantitative real-time PCR (qRT-PCR) was used for measuring
TERT
gene expression. Telomere length (TL) was measured using the Quantitative Fluorescence In Situ Hybridization (Q-FISH) technique. In silico analysis was performed for the prediction of the pathogenicity of resultant variants.
Results
Sequencing of
MPL
,
TERT
, and
TERC
genes identified 26 variants. Eleven variants were identified in the
MPL
gene. Three of them are pathogenic: two missense c.305 G>A, c.1589 C>T and one splice site g.9130T>G.
TERT
gene sequencing showed thirteen variants, among them, four novel c.484G>A, c.499G>A, c.512G>A, c.3164C>G and two previously reported c.835G>A, c.2031C>T were predicted to be pathogenic. Two variants were characterized within the
TERC
gene; n.514A>G and n.463 C>T.
TERT
gene expression was downregulated in 70% of studied patients and the Q-FISH technique detected telomere shortening in 82.5% of patients.
Conclusions
Twenty-six pathogenic and benign variants within the
TERC
,
TERT
, and
MPL
genes were identified among the studied AA patients that were in several cases associated with shortened telomeres and/or lower
TERT
gene expression. Genotype/phenotype correlation in AA patients is of great importance in explaining the disease severity and guiding therapeutic decisions.
PDF
