Fluorobenzene probes for protein profiling through selective cysteine labeling have been developed by rational reactivity tuning. Tuning was achieved by selecting an electron‐withdrawing para ...substituent in combination with variation of the number of fluorine substituents. Optimized probes chemoselectively arylated cysteine residues in proteins under aqueous conditions. Probes linked to azide, biotin, or a fluorophore were applicable to labeling of eGFP and albumin. Selective inhibition of cysteine proteases was also demonstrated with the probes. Additionally, probes were tuned for site‐selective labeling of cysteine residues and for activity‐based protein profiling in cell lysates.
Tuning in to cysteine: The reactivity of chemical probes for selective cysteine S‐arylation in proteins was tuned in a two‐dimensional fashion according to the number of fluorine substituents (see scheme) and the nature of an additional substituent. The optimized probes were suitable for protein labeling, the selective inhibition of cysteine proteases, and activity‐based protein profiling in cell lysates.
•Chemical composition and nutritional evaluation of Acacia tortilis seeds were studied.•The results indicated that the seeds are useful as a new source of food ingredients.•Antinutrients including ...phytic acid, tannins and trypsin inhibitor were detected.•The seed flour had functional properties make it suitable for several food formulations.
Chemical composition and nutritional evaluation as well as physicochemical and functional properties of seed flour of Acacia tortilis (Forssk.) Hayne ssp. raddiana were studied. The results indicated that seeds contained 5.30% moisture, 3.99% ash, 9.19% fat, 14.31% fiber, 27.21% protein and 45.30% carbohydrates. Potassium was the predominant element followed by calcium and then phosphorous. Phytic acid, tannins and trypsin inhibitor as antinutrients were detected. The amino acid profile compared well with FAO/WHO recommended pattern except for cystine/methionine, isoleucine, tyrosine/phenylalanine, lysine and threonine. Also, the first limiting amino acid was lysine. Fatty acid composition showed that linoleic acid was the major fatty acid, followed by palmitic, stearic, oleic and arachidic acids. The seed oil showed absorbance in the ultraviolet ranges, thus it can be used as a broad spectrum UV protectant. For physicochemical and functional properties, acacia seeds flour had excellent water holding index, swelling index, foaming capacity and foam stability.
Hepatocyte injury is assessed by serum aspartate transaminase and alanine transaminase estimation. In psychiatric populations, antipsychotic drugs (AD) are culprit in hepatic dysfunction. To assess ...transaminitis among psychiatric patients treated by AD. This cross-sectional study was conducted in Zagazig University Hospitals in Egypt, from December 2022 to February 2023. A total of 135 adult patients aged ≥ 18 years, were diagnosed with psychiatric disorders after exclusion of patients receiving any hepatotoxic drugs, viral hepatitis, having chronic liver or kidney diseases, diabetes mellitus, mental retardation, and pregnant females. Among the 135 patients, 104 (77.0%) were males. Their age was 32 ± 9, The most popular used class of AD was atypical AD 84 (62.2%). The overall incidence of transaminitis among patients receiving AD was 23/135 (17.04%) of patients; 13 (56.5%) were on atypical AD compared to 10 (43.5%) patients receiving combined AD, without any statistically significant difference. The use of AD in patients with psychiatric disorders is potentially safe with minimal transaminitis (<one-fold elevation). However, baseline and regular monitoring of serum aminotransferase is recommended during treatment.
In this report, we solved the advection–diffusion equation under pollutants deposition on the ground surface, taking wind speed and vertical diffusion depend on the vertical height. Also, we ...estimated a simple diffusion model from point source in an urban atmosphere and the conservative material with downwind was evaluated. Then, we calculated the extreme ground-level concentration as a function of stack height and plume rise in two cases. Comparison between the proposed models and the emission from the Egyptian Atomic Research Reactor at Inshas had been done. Lastly, we discussed the results in this report.
Abstract
Background
There are many surgical approaches which described extent of resection of the colon for adequate surgical management of splenic flexure cancer, but up till now there is no ...established surgical procedure, this is because the presence of double lymphatic drainage of the mesenteric vessels. Segmental resection of the colon for the management of splenic flexure cancer was a recently accepted surgical procedure.
Objective
In the present study, we aimed to compare three surgical management techniques to clarify the best management approach of Egyptian patients with splenic flexure cancer regarding operative, clinical, and oncological outcomes: segmental resection, and extended left or right hemicolectomy,.
Materials and Methods
In the present study, we included 90 patients with splenic flexure cancer. Cases were divided into 3 groups. Each group included 30 patients in order to compare three surgical techniques: segmental resection, extended left hemicolectomy, and extended right hemicolectomy.
Results
We have found no statistically significant differences between the three included groups regarding operative findings, postoperative complications, local recurrence, distant recurrence, disease progression, recurrence-free survival rate, progression-free survival rate, and overall survival rate. The operative time was longer, and the number of lymph nodes was higher in the extended right hemicolectomy group (
p
< 0.001).
Conclusion
We have shown that segmental resection of the splenic flexure is surgically and clinically suitable for the adequate management of operable cases of carcinoma of the splenic flexure.
Metallopeptides that show efficiency and selectivity in peptide bond cleavage in water at room temperature and neutral conditions are presented. These small and versatile organozymes take advantage ...of metal‐coordinating building blocks that are strategically positioned centrally in a peptide backbone or in a peptide macrocycle. This approach provided peptide–metal complexes with scaffolds capable of utilizing the peptide functionality for productive binding of fluorogenic FRET peptide substrates, subsequently leading to highly selective peptide bond cleavage. The ligand chemistry has been optimized to provide an easy access to new metallo‐peptides with the ability to cleave previously inaccessible peptide bonds. Evolutionary principles of stepwise selection and variation offered by combinatorial methods were used and were guided by molecular modeling to develop catalytic metallo‐peptides that mimic metalloproteases.
Metallopeptides that cleave other peptides were identified by combinatorial on‐resin selection. These “organozymes”, containing phenanthroline metal binding sites, like proteases, cleave FRET peptide substrates with high substrate specificity. The cleavage involves formation of a tight binding transition state of hydrolysis. The principles outlined for organozymes design is a platform for development of catalytic drugs, for processing pathogens.
The composition and physicochemical properties of defatted acacia flour (DFAF), acacia protein concentrate (APC) and acacia protein isolate (API) were evaluated. The results indicated that API had ...lower, ash and fat content, than DFAF and APC. Also, significant difference in protein content was noticed among DFAF, APC and API (37.5, 63.7 and 91.8%, respectively). Acacia protein concentrate and isolates were good sources of essential amino acids except cystine and methionine. The physicochemical and functional properties of acacia protein improved with the processing of acacia into protein concentrate and protein isolate. The results of scanning electron micrographs showed that DFAF had a compact structure; protein concentrate were, flaky, and porous type, and protein isolate had intact flakes morphology.
Pelvic organ prolapse (POP) is the descent of the pelvic organs into or through the vaginal walls. Females who have prolapse have symptoms that interfere with their daily lives, sexual function, and ...exercise. POP can have a negative effect on one's sexuality and body image. This study attempted to assess the significance of core stability exercises vs. interferential therapy on the power of the pelvic floor muscles in females with prolapsed pelvic organs.
A randomized control trial was conducted on forty participants (aged between 40-60 years, diagnosed with mild degree pelvic organ prolapse participated in the research. Participants were randomly divided into two groups: (group A; n = 20) and (group B; n = 20). The participants were tested twice, before and after 12 weeks, during which group (A) performed core stability exercises while group (B) received interferential therapy. A modified Oxford grading scale and the perineometer were used to assess how the vaginal squeeze pressure changed.
The study's findings demonstrated that the modified Oxford grading scale values and vaginal squeeze pressure had a non-statistically significant difference (p-value ≥ 0.05) between both groups pre-treatment while had a statistically significant difference (p-value ≤ 0.05) between both groups post-treatment in favor of group A.
It was concluded that both training programs are efficient at strengthening the pelvic floor muscles, but the core stability exercises were more effective.
Egyptian Siwi dates dried using the open sun drying is exposed to different contaminants. So, the current study aims to use the closed solar dryer to improve Siwi date safety. The impact of washing ...and closed solar drying on the levels of microbial load, aflatoxins and heavy metals in Egyptian Siwi dates (ESD), in comparison to traditional open sun drying methods were examined. Two different drying techniques were employed to dry 300 kg of ESD. The microbial load was assessed following the two drying procedures. The levels of aflatoxins and heavy metals were analyzed using High-performance liquid chromatography (HPLC) and Inductively Coupled Plasma (ICP) techniques, respectively, after both drying methods. Additionally, the influence of storage time on the microbial load of the ESD was also evaluated using standard methods. The findings of the current study demonstrated that the closed solar drying significantly reduced the total bacterial and fungal counts by 96 % and 93 %, respectively, when compared to open sun-drying. No aflatoxins were detected in both fresh Siwi dates and Siwi dates dried using closed solar drying. However, after open sun drying, two aflatoxins; aflatoxin B1 (AFB1) and aflatoxin G1 (AFG1), were detected in the ESD, with concentrations of 0.95 and 0.23 μg kg−1, respectively. The closed solar drying significantly decreased the levels of lead (Pb), cadmium (Cd), copper (Cu), nickel (Ni), chromium (Cr), zinc (Zn), manganese (Mn), and iron (Fe) in the dried dates by 96 %, 94 %, 48 %, 71 %, 64 %, 4 %, 26 %, and 7 %, respectively, when compared to open sun drying. The stored Siwi dates that was exposed to the open sun drying showed a higher increase in bacterial (4.86 log CFU/g) and fungal (4.46 log CFU/g) counts. However, the stored Siwi dates that was exposed to the closed solar dryer showed a lower increase in bacterial (3.21 log CFU/g) and fungal (2.51 log CFU/g) counts. So, the duration of storage significantly impacted the microbial loads of the closed solar dried dates as compared to open sun drying. Overall, closed solar drying reduced the levels of investigated contaminants and extended the shelf life of ESD, thereby enhancing their safety for human consumption.
Fluorobenzene probes for protein profiling through selective cysteine labeling have been developed by rational reactivity tuning. Tuning was achieved by selecting an electron‐withdrawing para ...substituent in combination with variation of the number of fluorine substituents. Optimized probes chemoselectively arylated cysteine residues in proteins under aqueous conditions. Probes linked to azide, biotin, or a fluorophore were applicable to labeling of eGFP and albumin. Selective inhibition of cysteine proteases was also demonstrated with the probes. Additionally, probes were tuned for site‐selective labeling of cysteine residues and for activity‐based protein profiling in cell lysates.
Auf Cystein abstimmen: Die Reaktivität von chemischen Sonden für die selektive Arylierung am Cystein‐Schwefel in Proteinen wurde in zweidimensionaler Weise feinjustiert: über die Zahl der Fluorsubstituenten (siehe Schema) und über die Art eines zusätzlichen Substituenten. Die optimierten Sonden waren für die Proteinmarkierung, die selektive Hemmung von Cysteinproteasen und das aktivitätsbasierte Proteinprofilierung von Zelllysaten geeignet.
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