In this work we present the results of spore and pollen analysis of forest soils from the Zvenigorod biological station of Moscow State University (Moscow Region, Russia). A comparative analysis of ...forest soils formed on the site of historical fields of the XVIII–XIX centuries and beyond showed that a specific complex of pollen and spores remains in the residual arable horizons, characteristic only of soils that have passed through the stages of plowing and fallow. It includes pollen from cultivated cereals and arable weeds (buckwheat, cornflower blue), spores of the mace-shaped plaunus (Lycopodium clavatum), as well as spores of the mosses Riccia glauca and Anthoceros spp. The latter are exclusive indicators of fallows, since they are practically not found in other habitats. The identified pollen indicators can be used in landscape and archaeological research to interpret the data of spore-pollen analysis of cultural layers, buried soils, gully-ravine sediments. They can also be used to define the boundaries of ancient fields under modern vegetation.
The paper presents the results of spore-pollen analysis of 47 surface soil samples from the Middle Volga region, taken both in territories with vegetation close to zonal one and in heavily disturbed ...vegetation areas and agricultural lands. The analysis showed an ambiguous relationship between subrecent spectra and modern vegetation, both local and regional. This is explained by strong anthropogenic transformation of landscapes and the spread of conifer plantations, which form the basis of the modern “pollen rain”. Comparison of the soil subrecent spectra with the spectra of the buried soils uncovered during archaeological excavations in Bolgar showed that the soils of different archaeological eras differ in the composition of the pollen preserved in them, which reflects significant changes in the landscape over time. These changes are associated mainly with the change of the dominant types of land use. It is shown that the pollen spectra of the trampled open areas (roadsides, wastelands and cattle tracks) have characteristic distinctive features, which can be used in the interpretation of the results of archaeological-soil studies.
Peatlands play an important role in the global carbon cycle but have been exploited over many centuries, which reduces their carbon storage capacity. To investigate peatland development during the ...late Holocene and their restoration after peat extraction, we applied a multi-proxy paleoecological (pollen, plant macrofossils, testate amoebae, loss on ignition, peat humification, etc.) approach to undisturbed and floating vegetation mat deposits of the Gorenki peatland (Meshchera Lowlands, East European Plain). Peatland development started around 2550 before the common era (BCE) as a waterlogged eutrophic birch forest (terrestrial paludification) surrounded by a broadleaf forest. Around 2400 BCE, the peatland turned into an open mire with Sphagnum mosses, sedges, and willows. During 900–800 BCE, the mire transformed into a wet mesotrophic peatland surrounded by a spruce forest. The first human settlements and deforestation around 300–400 CE coincided with oligotrophization of the mire. The growth of the Slavic population in the region in 14th century CE caused transformation of indigenous spruce–broadleaf forests into croplands, and the mire became drier and forested. Since peat extraction was abandoned in the beginning of 20th century CE, the mire has undergone self-restoration starting with the formation of a Sphagnum cuspidatum/obtusum quagmire on the floating peat remains. The Sphagnum mat stabilized during 1960–2000 CE. During the last twenty years, agricultural activity decreased and pine forests were restored in the adjacent area; the floating mat became drier and more oligotrophic, which can lead to the formation of a bog in the absence of considerable anthropogenic impact.
The results of a paleoecological study of a peat deposit in Radomsky Mokh area (Smolensk Oblast, Krasninsky District) made it possible to reconstruct the history of the region's landscapes over the ...last 4 thousand years and identify its 4 contrasting periods. The reservoir was a lake at first, after which the pollen spectrum is dominated by pollen from native tree species. After a series of fires on the boundary of the Bronze and Early Iron Ages (3173–2969 14C calendar years ago) (2σ, 99.7%)) a forested mesotrophic swamp formed at the site of the lake, and pollen from pioneer trees with an admixture of alder started to dominate the pollen spectrum. At the third stage, the swamp became mesotrophic, and the landscapes around it become open, which is evidenced by an increase in the proportion of grasses and anthropogenic indicators in the pollen spectrum. The same period is marked with the appearance of pollen of cultivated grasses (2992–2912 simulated years ago), which coincides with the distribution of the monuments of the Dnieper-Dvinian culture in the study area. At the last stage, the swamp became oligotrophic, and a stable curve of cultivated grasses appeared (mid-1st Millennium AD), indicating the spread of agriculture in the study area. The absence of known archaeological sites in an 8-kilometer radius around Radomsky Mokh most likely indicates an insufficient archaeological study level of the area, whereas the economic development of the vicinity of the tract started at the boundary of the Bronze and Early Iron Ages.
The article presents the results of the study of the hillfort at Bolshie Algashi village (Chuvashia, Russia) conducted in 2016–17. The study provides new data on the cultural and economic dynamics in ...the region during the Bronze and Early Iron Ages. A mixed group of Khula-Syuch and Chirki population can be identified as pastoralists and, probably, also farmers, who developed floodplains and forestless landscapes of the elevated riverbanks. Our research, therefore, provides evidence of almost the oldest farming activity episode in the lower reaches of the Sura River. The Early Iron Age occupation of the site is associated with the Andreevka-Piseraly population, who used the site mostly for its military functions.
This work demonstrates the use of a modified mica to concentrate proteins, which is required for proteomic profiling of blood plasma by mass spectrometry (MS). The surface of mica substrates, which ...are routinely used in atomic force microscopy (AFM), was modified with a photocrosslinker to allow "irreversible" binding of proteins via covalent bond formation. This modified substrate was called the AFM chip. This study aimed to determine the role of the surface and crosslinker in the efficient concentration of various types of proteins in plasma over a wide concentration range. The substrate surface was modified with a 4-benzoylbenzoic acid N-succinimidyl ester (SuccBB) photocrosslinker, activated by UV irradiation. AFM chips were incubated with plasma samples from a healthy volunteer at various dilution ratios (10
X, 10
X, and 10
X). Control experiments were performed without UV irradiation to evaluate the contribution of physical protein adsorption to the concentration efficiency. AFM imaging confirmed the presence of protein layers on the chip surface after incubation with the samples. MS analysis of different samples indicated that the proteomic profile of the AFM-visualized layers contained common and unique proteins. In the working series of experiments, 228 proteins were identified on the chip surface for all samples, and 21 proteins were not identified in the control series. In the control series, a total of 220 proteins were identified on the chip surface, seven of which were not found in the working series. In plasma samples at various dilution ratios, a total of 146 proteins were identified without the concentration step, while 17 proteins were not detected in the series using AFM chips. The introduction of a concentration step using AFM chips allowed us to identify more proteins than in plasma samples without this step. We found that AFM chips with a modified surface facilitate the efficient concentration of proteins owing to the adsorption factor and the formation of covalent bonds between the proteins and the chip surface. The results of our study can be applied in the development of highly sensitive analytical systems for determining the complete composition of the plasma proteome.
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