This study deals with an extensive taxonomic reevaluation focusing on phylogenetic relationships and morphological characterization of Tubeufiales, especially those helicosporous hyphomycetes which ...are difficult to identify. Based on evidence from DNA sequence data and morphology, we introduce 13 new genera in the family Tubeufiaceae,
viz. Acanthotubeufia
,
Dematiohelicoma
,
Dematiohelicomyces
,
Dematiohelicosporum
,
Dematiotubeufia
,
Helicoarctatus
,
Helicohyalinum
,
Helicotruncatum
,
Neochlamydotubeufia
,
Neohelicoma
,
Pleurohelicosporium
,
Pseudohelicomyces
and
Pseudohelicoon
; transfer
Chaetosphaerulina
from Dothideomycetes genera
incertae sedis
, and
Artocarpomyces
and
Helicodochium
from Ascomycetes genera
incertae sedis
into Tubeufiaceae; introduce 52 new species,
viz. Berkleasmium fusiforme
,
B. longisporum
,
Chlamydotubeufia cylindrica
,
Dematiohelicosporum guttulatum
,
Helicoarctatus aquaticus
,
Helicodochium aquaticum
,
Helicohyalinum infundibulum
,
Helicoma aquaticum
,
H. brunneisporum
,
H. cocois
,
H. rufum
,
H. fusiforme
,
H. longisporum
,
H. multiseptatum
,
H. rubriappendiculatum
,
H. septoconstrictum
,
H. tectonae
,
Helicomyces hyalosporus
,
Helicosporium aquaticum
,
H. flavisporum
,
H. setiferum
,
H. vesicarium
,
H. viridiflavum
,
Neochlamydotubeufia fusiformis
,
Neohelicomyces hyalosporus
,
Neohelicosporium acrogenisporum
,
N. astrictum
,
N. ellipsoideum
,
N. irregulare
,
N. krabiense
,
N. laxisporum
,
N. ovoideum
,
Pleurohelicosporium parvisporum
,
Pseudohelicomyces aquaticus
,
P. hyalosporus
,
Tubeufia abundata
,
T. bambusicola
,
T. brevis
,
T. brunnea
,
T. chlamydospora
,
T. dictyospora
,
T. eccentrica
,
T. fangchengensis
,
T. hechiensis
,
T. inaequalis
,
T. krabiensis
,
T. rubra
,
T. sessilis
,
T. sympodihylospora
,
T. sympodilaxispora
,
T. taiwanensis
and
T. tratensis
; provide 43 new combinations,
viz. Acanthohelicospora guianensis
,
Acanthotubeufia filiforme
,
Berkleasmium aquatica
,
B. guangxiense
,
B. latisporum
,
B. thailandicum
,
Dematiohelicoma perelegans
,
D. pulchrum
,
Dematiohelicomyces helicosporus
,
Dematiotubeufia chiangraiensis
,
Helicohyalinum aquaticum
,
Helicoma elinorae
,
H. gigasporum
,
H. hongkongense
,
H. linderi
,
H. nematosporum
,
H. pannosum
,
H. serpentinum
,
Helicomyces chiayiensis
,
Helicotruncatum palmigenum
,
Neochlamydotubeufia khunkornensis
,
Neohelicoma fagacearum
,
Neohelicomyces pallidus
,
Neohelicosporium abuense
,
N. aurantiellum
,
N. griseum
,
N. morganii
,
N. myrtacearum
,
N. nizamabadense
,
N. sympodiophorum
,
N. taiwanense
,
N. vesiculiferum
,
Pseudohelicomyces indicus
,
P. paludosus
,
P. talbotii
,
Pseudohelicoon gigantisporum
,
P. subglobosum
,
Tubeufia dentophora
,
T. geniculata
,
T. lilliputea
,
T. machaerinae
,
T. sympodiophora
and
T. xylophila
; introduce 16 new records,
viz. Dictyospora thailandica
,
Helicomyces colligatus
,
H. torquatus
,
Neohelicosporium guangxiense
,
N. hyalosporum
,
N. parvisporum
,
Thaxteriellopsis lignicola
,
Tubeufia aquatica
,
T. chiangmaiensis
,
T. cylindrothecia
,
T. filiformis
,
T. guangxiensis
,
T. laxispora
,
T. parvispora
,
T. roseohelicospora
and
T. tectonae
. The taxonomy of
Helicoma
,
Helicomyces
and
Helicosporium
is revisited based on phylogenetic analyses and morphological evidence.
Neorhamphoria
is transferred to Bezerromycetaceae. Three species are excluded from the genus
Chlamydotubeufia
, twelve species from
Helicoma
, four species from
Helicomyces
, 25 species from
Helicosporium
, six species from
Neoacanthostigma
and one species from
Tubeufia
. A multi-gene phylogenetic tree based on maximum likelihood and Bayesian analyses of ITS, LSU, RPB2 and TEF1α sequence data of species of Tubeufiales is provided. Detailed descriptions and illustrations are provided, as well as the morphological comparison with similar taxa are explored. The checklist of accepted Tubeufiales species and re-organised Tubeufiales species are provided.
During the investigations of discomycetes in Yunnan, China, five species of
were discovered on decayed, decorticated oak trees or unidentified wood. All species have typical disc-like, large fruiting ...bodies with grey, brown or greyish-green colors. The ITS sequence analysis showed that they belong to
(Helotiaceae, Helotiales) and the LSU and ITS combination revealed a different topology within the genus. Four species,
,
,
and
were established as new species, and
was collected and described on oak woods. The pairwise homoplasy index (PHI) test results indicated that there is no significant genetic recombination (Φ
= 1.0) between all related species pairs. All the species described here are supported by descriptions, illustrations and multi-gene analyses.
Jatropha seeds can be used to produce high-quality biodiesel due to their high oil content. However, Jatropha produces low numbers of female flowers, which limits seed yield. Paclobutrazol (PCB), a ...plant growth retardant, can increase number of Jatropha female flowers and seed yield. However, the underlying mechanisms of flower development after PCB treatment are not well understood. To identify the critical genes associated with flower development, the transcriptome of flower buds following PCB treatment was analyzed. Scanning Electron Microscope (SEM) analysis revealed that the flower developmental stage between PCB-treated and control flower buds was similar. Based on the presence of sex organs, flower buds at 0, 4, and 24 h after treatment were chosen for global transcriptome analysis. In total, 100,597 unigenes were obtained, 174 of which were deemed as interesting based on their response to PCB treatment. Our analysis showed that the JcCKX5 and JcTSO1 genes were up-regulated at 4 h, suggesting roles in promoting organogenic capacity and ovule primordia formation in Jatropha. The JcNPGR2, JcMGP2-3, and JcHUA1 genes were down-regulated indicating that they may contribute to increased number of female flowers and amount of seed yield. Expression of cell division and cellulose biosynthesis-related genes, including JcGASA3, JcCycB3;1, JcCycP2;1, JcKNAT7, and JcCSLG3 was decreased, which might have caused the compacted inflorescences. This study represents the first report combining SEM-based morphology, qRT-PCR and transcriptome analysis of PCB-treated Jatropha flower buds at different stages of flower development.
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•First study examining effects of paclobutrazol (PCB) on Jatropha flower buds using RNA-seq, qRT-PCR, and SEM.•PCB application significantly increased female flower number and seed yield in Jatropha.•PCB affected the expression of genes involved in phytohormone biosynthesis, flower development, and cell division.•Up-regulation of JcCKX5 and JcTSO1 may promote total flower number in Jatropha.•Down-regulation of JcNPGR2, JcMGP2-3, and JcHUA1 may contribute to increased female flowers and seed yield.
Helicosporous hyphomycetes are a morphologically allied group of Tubeufiales. We introduce a new helicosporous genus,
Neohelicosporium
, with five new species,
Neohelicosporium aquaticum
,
N. ...guangxiense
,
N. hyalosporum
,
N. parvisporum
, and
N. thailandicum
, based on morphological and phylogenetic evidence. The
RPB2
protein gene data are provided to analyze their phylogeny in Tubeufiales. Phylogenetic analyses of combined ITS, LSU,
RPB2
, and
TEF1α
sequence data from 13 new isolates of
Neohelicosporium
provided evidence to support the establishment of the new taxa. The morphological characters of
Neohelicosporium
that differentiate it from other helicosporous species are compared and discussed.
Relationships between some Thai cultivars of pineapple (Ananas comosus) revealed by RAPD analysis Siam Popluechai, Mae Fah Luang University, Chiang Rai (Thailand). School of Science; Somrudee Onto, Mae Fah Luang University, Chiang Rai (Thailand). School of Science; Prapassorn Damrongkool Eungwanichayapant, Mae Fah Luang University, Chiang Rai (Thailand). School of Science
Wārasān Songkhlā Nakharin,
Nov-Dec 2007
Journal Article
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