The role of HPV in the carcinogenesis of intraepithelial and invasive anogenital lesions is currently well established. E6 and E7 oncoproteins of high-risk HPV genotypes are known to inactivate p53 ...and pRb pathways. Several studies have described an increased prevalence and recurrence of both cervical HPV infection and invasive cervical cancer among HIV-1 positive women compared to HIV-1 negative cases. For these reasons, cervical cancer is considered an AIDS-defining neoplasm. Unlike other AIDS-associated neoplasms, the occurrence of cervical cancer is independent of immune suppression. HIV-1 infection in patients with high grade precancerous lesions and invasive cervical cancers results in a therapy refractory and more aggressive disease phenotype, which is not yet well understood at the molecular level. An upregulation of HPV E6 and E7 gene expressions by HIV-1 proteins such as Tat has been documented by some authors. However, the role of HIV-1 in cervical carcinomas is still unclear. It is already known that HIV-1 Tat protein is able to influence cell cycle progression. Altogether, these facts led us to investigate the effects of Tat on the expression of cell cycle regulator genes. After transfection of HeLa cells with Tat, we analyzed the expression of cell cycle regulators from these cells by IHC and Real-time PCR. A significant reduction in the expression of cell cycle inhibitors of transcription and an increase in the levels of proliferation markers were observed. These results suggest that HIV-1 may enhance cervical carcinogenesis by promoting cell cycle progression. We also found that this HIV-1 Tat-induced cell proliferation was not dependent on the E2F family of transcription factors, and therefore postulate that Sp factors may be involved.
This study was designed to estimate the dietary intake of arsenic (As), cadmium (Cd), mercury (Hg), and lead (Pb) by the general population of Catalonia, Spain. The concentrations of these elements ...were determined in food samples randomly acquired in seven cities of Catalonia between June and August 2000. A total of 11 food groups were included in the study. As, Cd, Hg, and Pb levels were measured by ICP-MS and AAS. The dietary intake of these elements was determined by a total diet study. Calculations were carried out on the basis of recent data on the consumption of the selected food items. Trace element intake was estimated for five population groups: children, adolescents, male and female adults, and seniors. The highest dietary intakes of As (223.6 microg/day), Cd (15.7 microg/day), Hg (21.2 microg/day), and Pb (28.4 microg/day) corresponded to male adults. For all analyzed elements, fish and shellfish was the group showing the highest contribution to the respective intakes. In comparison with previous results, a general decrease in As, Cd, Hg, and Pb intake has occurred. The dietary intake of these elements was also compared with the provisional tolerable weekly intake (PTWI). Dietary intakes of As, Cd, Hg, and Pb by the population of Catalonia are currently well below the respective PTWIs.
The electric-field assisted deposition is successfully proposed as a method for the manufacturing of carbon nanostructured films with tunable properties, benefiting from the superimposition of ...electric fields on the thermophoretic deposition. Morphology, optical, and thermo-resistive properties of the carbon nanoparticle (CNP) films have been studied by UV–vis Absorption Spectroscopy, Scanning Electron Microscopy, Atomic Force Microscopy, and Current-Voltage analysis. In comparison to thermophoresis alone, the introduction of an electric field results in a six-fold increase in the deposition rate characterized by a non-linear film growth influenced by a three-fold augmentation in surface roughness and polarization effects. Notably, the surface morphology of the CNP films undergoes modification, exhibiting larger grains and a reduced optical band gap energy. Moreover, while maintaining a non-ohmic behaviour, the electric field plays a crucial role in increasing by about two orders of magnitude the electrical conductance of CNP films at ambient temperature. This effect is accompanied by a decrease in temperature sensitivity, attributed to the low and nearly temperature-independent activation energy for the tunneling of electrons in the percolative network. In summary, electric-field assisted deposition is a promising approach to tailor the thermal response of CNP films, which could be beneficial for the development of next-generation sensors.
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•Electric field-assisted thermophoretic deposition of flame-made CNP films is proposed.•Film morphology and electrical conductivity can be controlled.•Thermal response of CNP films can be tailored by electric field-assisted deposition.
Nitric Oxide (NO) has been linked to several cardiovascular, neurological and immunological physiological and pathological functions. Several studies have shown that the eNOS, nNOS and iNOS effects ...on cancer cell growth and proliferation are related to the upregulation of the Wnt pathway and have a central role during metastasis development. Recent studies suggest that cancer cells undergo metabolic reprogramming, which drives cancer cell growth and progression. The aim of this study was to observe the NOS activity in the pathogenesis of oral precancerous and cancerous lesions. The results showed changes in eNOS activity levels, which increased from healthy oral mucosa to oral squamous cell carcinoma SCC, through different dysplasia levels. The iNOS activity levels increased in precancerous lesions compared to healthy mucosa, where iNOS was absent, while it decreased in SCC lesions. Moreover, a gradual increase of nNOS activity together with the progression of the lesions was also found. These results may suggest how NO could play a critical role during pathogenesis, growth and development of precancerous lesions to cancer degeneration.
We depict the relative sea-level rise scenarios for the year 2100 from four areas of the Italian peninsula. Our estimates are based on the Rahmstorf (2007) and IPCC-AR5 reports 2013 for the RCP-8.5 ...scenarios (www.ipcc.ch) of climate change, adjusted for the rates of vertical land movements (isostasy and tectonics). These latter are inferred from the elevation of MIS 5.5 deposits and from late Holocene sea-level indicators, matched against sea-level predictions for the same periods using the glacio-hydro-isostatic model of Lambeck et al. (2011). We focus on a variety of tectonic settings: the subsiding North Adriatic coast (including the Venice lagoon), two tectonically stable Sardinia coastal plains (Oristano and Cagliari), and the slightly uplifting Taranto coastal plain, in Apulia. Maps of flooding scenarios are shown on high-resolution Digital Terrain Models mostly based on Lidar data. The expected relative sea-level rise by 2100 will change dramatically the present-day morphology, potentially flooding up to about 5500 km2 of coastal plains at elevations close to present-day sea level.
The subsequent loss of land will impact the environment and local infrastructures, suggesting land planners and decision makers to take into account these scenarios for a cognizant coastal management. Our method developed for the Italian coast can be applied worldwide in other coastal areas expected to be affected by marine ingression due to global climate change.
Flame synthesis of Carbon NanoParticle (CNP) films is gaining strong interest for novel industrial applications because of the easy tuning of the operating conditions that enables accurate control of ...the chemical and physical properties of the produced CNPs. This work proposes a novelty in the synthesis of CNP films, namely the possibility of applying electric fields in flame to trigger electrophoretic deposition phenomena. In this way, it is possible to add another degree of freedom to the harvesting process and potentially modify the properties of the CNP films, without changing the operating flame conditions. To investigate the physical mechanisms governing the thermo-electrophoretic deposition of CNPs, a numerical model to simulate the particle dynamics close to the collecting substrate has been developed, and experiments have been carried out to provide highly controlled test conditions that can be used to support model validation. The experimental results consist of Atomic Force Microscopy (AFM) measurements to determine the number of particles deposited after a controlled harvesting condition as a function of the applied electrophoretic force, imposed on the substrate by means of a DC voltage varied from 0 to -3kV. The AFM shows that the amount of deposited material increases up to six times when passing from an uncharged to the -3kV charged case. The model predictions are highly consistent with the AFM measurements and pointed out that the electric field in flame significantly alters the CNP deposition velocities and impact angles, which are likely to affect the properties of the film.
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•Proposed model is able to reproduce AFM results for particle deposition.•Electrophoresis is effective in harvesting naturally charged particles in flame.•Electrophoresis is the dominant phenomenon when active for particle deposition.•Electrophoresis increases up to 6 times the collection rate of charged particles.•Electrophoresis increases impact velocity of collected particles on substrate.
Urocortin (UCN) gene expression and synthesis have been reported in epithelial and stromal cells of the human endometrium. In this study we evaluated (i) UCN messenger RNA (mRNA) expression and ...peptide production in uterine specimens collected throughout the endometrial cycle, (ii) UCN secretion after decidualization of cultured human endometrial stromal cells (HESCs) and (iii) the effect of UCN on endometrial decidualization. METHODS: HESCs were isolated from samples of human endometrium collected from healthy patients with normal menstrual cycle and cultured in presence of cAMP, 17-beta-estradiol (E2) + medroxyprogesterone acetate (MPA) and UCN. UCN levels were measured in endometrial extracts by an enzyme immunoassay, and changes of endometrial UCN mRNA expression were measured by RT–PCR analysis. RESULTS: UCN peptide concentrations and mRNA expression were highest in the secretory phase of the menstrual cycle (P < 0.001, late secretory versus early and late proliferative phase) and higher in the late than the early secretory phase (P < 0.01). After decidualization of HESC with cAMP or E2 + MPA, UCN levels rose in parallel with prolactin concentrations by days 6 (P < 0.01, for all). Finally, the addition of UCN to HESCs, with or without E2 + MPA, induced the release of prolactin. CONCLUSIONS: The evidence that (i) UCN is highly expressed in the secretory phase of the endometrial cycle; (ii) cAMP and E2 + MPA modulate secretion of UCN and (iii) UCN induces HESCs decidualization together suggest a possible role for UCN in endometrial physiology.
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