Skeletal muscle from embryonic day 20 (E20) was combined with E15 rat spinal cord in the anterior chamber of the eye of adult albino rats. The two grafts were either transplanted concomitantly or ...sequentially, in which case muscle tissue was added 4 months after the spinal cord. Control groups received a single graft of either spinal cord or skeletal muscle. Survival and intraocular growth were observed through the cornea. After maturation in oculo, the double grafts were examined immunohistologically utilizing antisera to neurofilament (NF) and acetylcholinesterase (AChE). The grafts were also evaluated using electrical stimulation to determine functional connectivity. The spinal cord and skeletal muscle grafts were found to exert reciprocal trophic effects on each other, evidenced as a larger muscle mass in skeletal muscle grafts allowed to develop in the presence of spinal cord tissue, and a larger volume of spinal cord grafts allowed to develop together with a skeletal muscle graft, respectively. Immunohistochemistry revealed NF-positive nerve fibers leaving the spinal cord graft and entering the muscle tissue. AChE-positive endplates developed in the muscle grafts. Electrical stimulation of the spinal cord part of double-graft combinations generally elicited contractile responses in specific areas of the muscle cograft. These results demonstrate both structural and functional connections between grafts of spinal cord and skeletal muscle tissue in vivo. The fact that such connections were also established between a mature (adult) spinal cord graft and fetal skeletal muscle tissue suggests that some alpha-motoneurons are able to survive for many months in the intraocular grafts without an appropriate target, and that they are able to subsequently innervate skeletal muscle targets.
Biochemical studies indicate that ethanol (EtOH) will facilitate the activation of the GABAA/Cl- channel, and behavioral studies demonstrate that EtOH-induced sedative and incoordinating effects can ...be potentiated by GABA mimetics and blocked by GABA antagonists. It has been difficult, however, to demonstrate an EtOH-induced potentiation of the depressant electrophysiological effects of locally applied GABA in mammalian brain in vivo. Similarly, in this study, local EtOH applications only infrequently caused potentiations of the depressant effects of microiontophoretically applied GABA on cerebellar Purkinje neurons, and this interaction was modest when present. The predominant interaction of locally applied EtOH was an antagonism of GABA-induced depressions of neuronal activity. However, the GABAA receptor antagonist bicuculline reversibly and apparently competitively blocked the depressant effects of locally applied EtOH on single cerebellar Purkinje neurons. Our data suggest that EtOH potentiation of GABA responses alone is insufficient to account for EtOH-induced depressions of cerebellar Purkinje neurons. However, these data clearly imply that activation of a GABAA receptor is required for the expression of EtOH-induced depressions of neuronal activity in this brain area. It is less clear how lower, nondepressant doses of EtOH interact with GABA mechanisms. We hypothesize that either the GABAA receptor mechanism must be sensitized to the potentiative effects of EtOH through the influences of neuromodulatory and/or hormonal regulation, or that EtOH interacts directly with these regulatory processes.
A successful migration from current C-band based optical networks to a multiband scenario primarily depends on the development of solutions that can reliably measure physical properties of optical ...links over broad spectral transmission windows. Additionally, these solutions must be capable of delivering wavelength-dependent and spatially-resolved indicators that can empower network operators to identify faults before they lead to severe service disruptions. Recently, the exploitation of receiver based digital signal processing as a tool for optical performance monitoring has gained tremendous popularity. One successful example is the so-called in-situ power profile estimator, which can reconstruct the per-channel longitudinal power profile along the optical fiber link solely processing the received signal samples. In this work, we propose a novel application for the in-situ power profile estimator by harnessing it on multiple wavelengths to accurately estimate the spectral gain profile of C+L-band in-line Erbium-doped fiber amplifiers deployed in a 280-km single mode fiber link. Furthermore, we show how this scheme can be efficiently used to detect amplification-related anomalies, such as gain tilt and narrowband gain compression. In our measurements, we achieved a sub-dB estimation accuracy by comparing the proposed gain extraction approach with the back-to-back characterization obtained from an optical spectrum analyzer.
Previous data indicated that bath-application of nicotine to mouse hippocampal slices resulted in a concentration-dependent increase in the amplitude of the orthodromic population spike and the ...appearance of multiple population spikes in the CA1 pyramidal cell layer. d-Tubocurarine (4-100 microM), alpha-bungarotoxin (10-160 microM), and atropine (40-200 microM) had similar effects, although for alpha-bungarotoxin these excitatory effects were transient. Mecamylamine (1.6-3.2 mM) inhibited the population spike, while hexamethonium (3.2 mM) had no effect. These cholinergic antagonists were tested for their ability to block excitatory effects of nicotine (800 microM) at antagonist concentrations which were at or near threshold for intrinsic effects. Of the 5 antagonists tested, only mecamylamine (400 microM) effectively inhibited the nicotine-induced increase of the population spike amplitude and the appearance of multiple population spikes. These results suggest that nicotine exerts electrophysiological effects via a subclass of nicotinic cholinergic receptors that is neither neuromuscular nor ganglionic in the classical sense; these brain nicotinic receptors are sensitive to mecamylamine, but not to hexamethonium, alpha-bungarotoxin, or D-tubocurarine.
Next-generation optical communication networks aim to vastly increase capacity by exploiting a larger optical transmission window covering the S-C-L-band. Simultaneously, the clear market trend is to ...maximize capacity per wavelength to reduce operational costs. This approach requires an increase in spectral efficiency, resulting in stringent requirements on the transceivers, which may not be satisfied in a multi-band (MB) scenario by current commercial components designed for operation in C-band. Transceiver specifications for MB operation can be relaxed through additional digital signal processing (DSP), at the cost of additional complexity, and by more resource-intensive calibration procedures. In this context, we experimentally characterize the wavelength-dependent frequency-resolved in-phase/quadrature (I/Q) imbalance of a standard C-band IQ-modulator and coherent receiver operating in an S-C-L-band system utilizing receiver-side DSP. This operation allows us to understand the nature of the wavelength-dependency of I/Q imbalance in MB systems. In the considered scenario, we validate the effectiveness of a cost-effective strategy for transceiver impairments mitigation and monitoring based on standard wavelength-independent calibration and reduced-complexity DSP.
The development of efficient anomaly detection schemes is a key element for the implementation of autonomous optical networks as they can help telecom operators to automate the location of defective ...devices and track the overall performance of the network infrastructure. In that regard, the exploitation of receiver based digital signal processing (DSP) for optical performance monitoring has shown to be a promising enabler for detection of spatially resolved and wavelength-dependent properties and anomalies in optical fiber links. In this work, we study the benefits of applying DSP-based longitudinal power estimation on multiple wavelength division multiplexing (WDM) channels allocated in the optical grid to infer wavelength-wise characteristics of a C+L-band optical line system. In that context, we show that the applied scheme can successfully recreate a visualization of the spatial evolution of the gain tilt imposed by in-line optical amplifiers. Additionally, we propose the utilization of advanced DSP tools based on wavelet-denoising to enhance the performance of an anomaly detection approach. The proposed method not only can improve accuracy of the fault location, by reducing positioning uncertainty, but it also delivers more uniform readings of the anomaly signatures.
Bath-application of nicotine (800 microM) to mouse hippocampal slices resulted in an increase in the amplitude of the population spike and the appearance of multiple population spikes in the CA1 ...pyramidal cell layer. Similar effects were observed after perfusion of the GABAA antagonist bicuculline methiodide (2 microM) and the glutamate decarboxylase inhibitor L-C-allylglycine (4 mM). These apparently excitatory effects of nicotine (800 microM) could be reversed by bath-application of gamma-aminobutyric acid (GABA; 400 microM), as well as by the GABA uptake inhibitor nipecotic acid (5 mM) and the benzodiazepine flurazepam (4 microM). Nicotine did not alter binding of 3HGABA or 3Hflunitrazepam to whole brain plasma membranes. The results are consistent with the hypothesis that the electrophysiological effects of nicotine on CA1 pyramidal cell excitability is mediated by disruption of GABAergic transmission.
Previous studies indicated that the excitatory effects of nicotine may be mediated via interference with GABAergic transmission. Here, several variants of the paired-pulse paradigm were employed to ...ascertain whether nicotine interferes with endogenous inhibitory circuits in the hippocampus. Nicotine attenuated the inhibition evoked by antidromic (alvear) stimulation in the CA1 region in a concentration-dependent manner (EC50 = 60-75 microM). This same phenomenon was also observed for the GABAA receptor antagonist bicuculline (0.1 microM). Orthodromic-orthodromic paired-pulse paradigms were found to be unsuitable for investigating the effects of epileptogenic agents such as nicotine and bicuculline on endogenous inhibition.
The nitrogen excretory metabolism of the myxomycete Physarum polycephalum was studied. When cultured in partially defined broth medium or on agar, the principal excretory product was ammonia ...nitrogen. A small, variable quantity of urea was excreted in liquid culture. No uric acid or other purines were detected in the cultures. When microplasmodia were incubated with sodium 14Cbicarbonate, radioisotope was incorporated into citrulline, arginine, and urea. Incubation with L-carbamoyl-14Ccitrulline yielded labelled arginine, urea, and CO2. Substantial urease activity was found in extracts of the microplasmodia. These results, in conjunction with the lack of an absolute nutritional requirement for arginine, provide evidence that Physarum has a functional arginine biosynthetic pathway, an arginase, and a urease.
We present a new, accurate, low complexity channel modelling methodology for LiFi in realistic indoor scenarios. A LIDAR scanner is used to capture the 3D environment in which the LiFi system is to ...be deployed. Next, the generated 3D point cloud data is pre-processed to determine the reflectance parameters of the walls and objects in the room. This is easier and more realistic than the manual definition of the environment, which is the current state of the art. As an additional innovation, the complexity of the channel modelling is reduced by: 1) modelling the line-of-sight and initial reflections precisely in the frequency-domain; 2) using a well-established analytical model based on the integrating sphere for all higher-order diffuse reflections. All steps together yield a substantially simplified channel modelling approach and model the links between multiple optical frontends and multiple mobile devices realistically. As a validation of our new approach, we compare measurements and simulations in two indoor scenarios: an empty room and a conference room with furniture. Simulations and measurements show excellent agreement with a mean square error below 3 percent. Moreover, we evaluate the performance of a distributed multiuser multiple-input multiple-output (MIMO) link and found excellent agreement between the model and measurements. Finally, we discuss the fundamental trade-off between complexity and model error, which depends on the scenario.
PDF
