Advanced wound scaffolds that integrate active substances to treat chronic wounds have gained significant recent attention. While wound scaffolds and advanced functionalities have previously been ...incorporated into one medical device, the wirelessly triggered release of active substances has remained the focus of many research endeavors. To combine multiple functions including light‐triggered activation, antiseptic, angiogenic, and moisturizing properties, a 3D printed hydrogel patch encapsulating vascular endothelial growth factor (VEGF) decorated with photoactive and antibacterial tetrapodal zinc oxide (t‐ZnO) microparticles is developed. To achieve the smart release of VEGF, t‐ZnO is modified by chemical treatment and activated through ultraviolet/visible light exposure. This process would also make the surface rough and improve protein adhesion. The elastic modulus and degradation behavior of the composite hydrogels, which must match the wound healing process, are adjusted by changing t‐ZnO concentrations. The t‐ZnO‐laden composite hydrogels can be printed with any desired micropattern to potentially create a modular elution of various growth factors. The VEGF‐decorated t‐ZnO‐laden hydrogel patches show low cytotoxicity and improved angiogenic properties while maintaining antibacterial functions in vitro. In vivo tests show promising results for the printed wound patches, with less immunogenicity and enhanced wound healing.
To treat chronic wounds, a smart wound scaffold based on growth factors decorated tetrapodal zinc oxide (t‑ZnO)‐laden 3D printed hydrogel patches, which possess light‐triggered activation, anti‐bacterial, angiogenic, and tissue regeneration properties is reported. Through in vivo tests, the multi‐functional wound scaffolds show less immunogenicity and enhance wound healing, which make them interesting candidates for future smart wound dressing platforms.
Accelerometry is a recent method used to quantify workload in team sports. A rapidly increasing number of studies supports the practical implementation of accelerometry monitoring to regulate and ...optimize training schemes. Therefore, the purposes of this study were: (1) to reflect the current state of knowledge about accelerometry as a method of workload monitoring in invasion team sports according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines, and (2) to conclude recommendations for application and scientific investigations. The Web of Science, PubMed and Scopus databases were searched for relevant published studies according to the following keywords: "accelerometry" or "accelerometer" or "microtechnology" or "inertial devices", and "load" or "workload", and "sport". Of the 1383 studies initially identified, 118 were selected for a full review. The main results indicate that the most frequent findings were (i) devices' body location: scapulae; (b) devices brand: Catapult Sports; (iii) variables: PlayerLoadTM and its variations; (iv) sports: rugby, Australian football, soccer and basketball; (v) sex: male; (vi) competition level: professional and elite; and (vii) context: separate training or competition. A great number of variables and devices from various companies make the comparability between findings difficult; unification is required. Although the most common location is at scapulae because of its optimal signal reception for time-motion analysis, new methods for multi-location skills and locomotion assessment without losing tracking accuracy should be developed.
We present an experimental demonstration of strong coupling between a surface plasmon propagating on a planar silver thin film and the lowest excited state of CdSe nanocrystals. Attenuated total ...reflection measurements demonstrate the formation of plasmon-exciton mixed states, characterized by a Rabi splitting of approximately 112 meV at room temperature. Such a coherent interaction has the potential for the development of nonlinear plasmonic devices, and furthermore, this system is akin to those studied in cavity quantum electrodynamics, thus offering the possibility to study the regime of strong light-matter coupling in semiconductor nanocrystals under easily accessible experimental conditions.
1366 I. 1366 II. 1367 III. 1368 IV. 1368 V. 1369 VI. 1370 VII. 1372 VIII. 1372 IX. 1376 X. 1377 1377 References 1377 SUMMARY: The aim of producing sustainable liquid biofuels and chemicals from ...lignocellulosic biomass remains high on the sustainability agenda, but is challenged by the costs of producing fermentable sugars from these materials. Sugars from plant biomass can be fermented to alcohols or even alkanes, creating a liquid fuel in which carbon released on combustion is balanced by its photosynthetic capture. Large amounts of sugar are present in the woody, nonfood parts of crops and could be used for fuel production without compromising global food security. However, the sugar in woody biomass is locked up in the complex and recalcitrant lignocellulosic plant cell wall, making it difficult and expensive to extract. In this paper, we review what is known about the major polymeric components of woody plant biomass, with an emphasis on the molecular interactions that contribute to its recalcitrance to enzymatic digestion. In addition, we review the extensive research that has been carried out in order to understand and reduce lignocellulose recalcitrance and enable more cost‐effective production of fuel from woody plant biomass.
We present results from a set of numerical simulations aimed at exploring the mechanism of coronal mass ejection (CME) suppression in active stars by an overlying large-scale magnetic field. We use a ...state-of-the-art 3D magnetohydrodynamic code that considers a self-consistent coupling between an Alfvén wave-driven stellar wind solution, and a first-principles CME model based on the eruption of a flux rope anchored to a mixed-polarity region. By replicating the driving conditions used in simulations of strong solar CMEs, we show that a large-scale dipolar magnetic field of 75 G is able to fully confine eruptions within the stellar corona. Our simulations also consider CMEs exceeding the magnetic energy used in solar studies, which are able to escape the large-scale magnetic field confinement. The analysis includes a qualitative and quantitative description of the simulated CMEs and their dynamics, which reveals a drastic reduction of the radial speed caused by the overlying magnetic field. With the aid of recent observational studies, we place our numerical results in the context of solar and stellar flaring events. In this way, we find that this particular large-scale magnetic field configuration establishes a suppression threshold around ∼3 × 1032 erg in the CME kinetic energy. Extending the solar flare-CME relations to other stars, such CME kinetic energies could be typically achieved during erupting flaring events with total energies larger than 6 × 1032 erg (GOES class ∼X70).
We report a methodology to calculate the free energy of a shape transformation in a lipid membrane directly from a molecular dynamics simulation. The bilayer need not be homogeneous or symmetric and ...can be atomically detailed or coarse grained. The method is based on a collective variable that quantifies the similarity between the membrane and a set of predefined density distributions. Enhanced sampling of this “Multi‐Map” variable re‐shapes the bilayer and permits the derivation of the corresponding potential of mean force. Calculated energies thus reflect the dynamic interplay of atoms and molecules, rather than postulated effects. Evaluation of deformations of different shape, amplitude, and range demonstrates that the macroscopic bending modulus assumed by the Helfrich–Canham model is increasingly unsuitable below the 100‐Å scale. In this range of major biological significance, direct free‐energy calculations reveal a much greater plasticity. We also quantify the stiffening effect of cholesterol on bilayers of different composition and compare with experiments. Lastly, we illustrate how this approach facilitates analysis of other solvent reorganization processes, such as hydrophobic hydration. Published 2019. This article is a U.S. Government work and is in the public domain in the USA.
A simulation methodology to calculate the potential of mean force of an arbitrary deformation of a lipid bilayer is presented. The membrane need not be homogeneous or symmetric, and lipids might be atomically detailed or coarse‐grained. The central element is a grid‐based collective variable denominated Multi‐Map, which quantifies the membrane similarity to a set of density fields reflecting the transformation of interest. The proposed approach may be adapted to study other solvent reorganization processes.
We used electron cryotomography of mitochondrial membranes from wild-type and mutant Saccharomyces cerevisiae to investigate the structure and organization of ATP synthase dimers in situ. Subtomogram ...averaging of the dimers to 3.7 nm resolution revealed a V-shaped structure of twofold symmetry, with an angle of 86° between monomers. The central and peripheral stalks are well resolved. The monomers interact within the membrane at the base of the peripheral stalks. In wild-type mitochondria ATP synthase dimers are found in rows along the highly curved cristae ridges, and appear to be crucial for membrane morphology. Strains deficient in the dimer-specific subunits e and g or the first transmembrane helix of subunit 4 lack both dimers and lamellar cristae. Instead, cristae are either absent or balloon-shaped, with ATP synthase monomers distributed randomly in the membrane. Computer simulations indicate that isolated dimers induce a plastic deformation in the lipid bilayer, which is partially relieved by their side-by-side association. We propose that the assembly of ATP synthase dimer rows is driven by the reduction in the membrane elastic energy, rather than by direct protein contacts, and that the dimer rows enable the formation of highly curved ridges in mitochondrial cristae.
Solar coronal mass ejections (CMEs) and flares have a statistically well-defined relationship, with more energetic X-ray flares corresponding to faster and more massive CMEs. How this relationship ...extends to more magnetically active stars is a subject of open research. Here we study the most probable stellar CME candidates associated with flares captured in the literature to date, all of which were observed on magnetically active stars. We use a simple CME model to derive masses and kinetic energies from observed quantities and transform associated flare data to the Geostationary Operational Environmental Satellite 1-8 band. Derived CME masses range from ∼1015 to 1022 g. Associated flare X-ray energies range from 1031 to 1037 erg. Stellar CME masses as a function of associated flare energy generally lie along or below the extrapolated mean for solar events. In contrast, CME kinetic energies lie below the analogous solar extrapolation by roughly 2 orders of magnitude, indicating approximate parity between flare X-ray and CME kinetic energies. These results suggest that the CMEs associated with very energetic flares on active stars are more limited in terms of the ejecta velocity than the ejecta mass, possibly because of the restraining influence of strong overlying magnetic fields and stellar wind drag. Lower CME kinetic energies and velocities present a more optimistic scenario for the effects of CME impacts on exoplanets in close proximity to active stellar hosts.
Attack by herbivores is a major biotic stress limiting the soybean crop production. Plant defenses against caterpillars include the production of secondary metabolites such as flavonoids, which ...constitute a diverse group of plant secondary metabolites. Thus, a more discriminate metabolic profiling between genotypes are important for a more comprehensive and reliable characterization of soybean resistance. Therefore, in this study a non-targeted LC/MS-based for analysis of flavonoid profiles of soybean genotypes contrasting to the resistance to A. gemmatalis was applied. Clustering analysis revealed profiles highly distinct between the susceptible UFV 105 AP and the resistant IAC 17 genotypes. This comparative approach enables to identify directly from leaf extract some new compounds related to resistance, some of which were present in higher abundance specifically in the IAC 17 genotype: four Quercetin conjugates, Rutin (Quercetin 3-O-Rutinoside), Quercetin-3,7-O- di-glucoside, Quercetin-3-O-rhamnosylglycoside-7-O-glucoside and Quercetin-3-O-rhamnopyranosyl-glucopyranoside-rhamnopyranoside; two Genistein conjugates, Genistein-7-O-diglucoside-dimalonylated and Genistein-7-O-6-O-malonylglucoside; and one Daidzein conjugate, Daidzein-7-O-Glucoside-malonate. The most abundant flavonoid glycoconjugates in soybean leaves belongs to Quercetin and Kaempferol classes. However, only one from the identified compounds was classified as a Kaempferol. The Kaempferol-3-O-L-rhamnopyranosyl-glucopyranoside showed high abundance in the resistant genotype IAC 17. The metabolic profiles generated by LC/MS allowed the reconstruction of the flavonoid biosynthetic pathways, which revealed a constitutive character for herbivory resistance in the resistant genotype IAC-17 and a metabolic regulation for the rechanneling of Quercetin, Kaempferol and Genistein conjugates in soybean. Highest relative abundances were detected for glyconjugates, such as Rutin, Quercetin 3-O-rhamnosylglycoside-7-O-glucoside and Quercitin-3-O-rhamnopyranosyl-glucopyranoside-rhamnopyranoside in the leaves of the resistant genotype.
Motivated by the recent work of Brzemiński, Motyka, Sadzikowski and Stebel in 1, where forward Drell–Yan production is studied in proton–proton collisions at the LHC, we improve their calculation by ...introducing an unintegrated gluon density obtained in 2 from a fit to combined HERA data at small values of Bjorken x. This gluon density was calculated within the BFKL formalism at next-to-leading order with collinear corrections. We show that it generates a good description of the forward Drell–Yan cross section dependence on the invariant mass of the lepton pair both for LHCb and ATLAS data.
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