Thirty-six strains of yellow-pigmented Pseudomonas from clinical as well as non-clinical material and 11 reference strains of Pseudomonas were investigated by means of conventional bacteriological ...methods (a total of 53 different tests). Eighteen of the 36 yellow-pigmented strains could be classified as P. paucimobilis. Apart from the presence of lipid inclusions on beta-hydroxybutyrate, hydrolysis of DNA, and Tween 80 our results showed a high degree of accordance with other investigations. Eight strains showed characteristics compatible with inclusion in the CDC VE group; one orange-yellow strain showed the characteristics of P. vesicularis, and one was a pyoverdin negative, yellow P. putida. Eight strains remained unidentified. Strains of P. paucimobilis were most often resistant to antibiotics used for P. aeruginosa infections (viz. piperacillin, cefsulodin, ceftazidime) while the strains of the CDC VE group were often susceptible. Most strains were susceptible to the aminoglycosides. The difficulties in distinguishing yellow-pigmented strains of Pseudomonas from Flavobacterium spp. or Xanthomonas spp. are discussed.
Over a 6-year period we detected 30 clinical infections caused by halophilic vibrios in a restricted geographical area. Vibrio parahaemolyticus infections were found in 13 patients (3 with wound ...infections, 10 with ear infections), and Vibrio alginolyticus infections in 17 patients, all of whom had ear infections. From 1987 to 1990, infections caused by marine vibrios were found in at most 4 cases annually, in 1992 in 6 instances, whereas in a 5-month period in 1991 we experienced 15 cases of extra-intestinal infections. 15 of the infections found in 1991 and 1992 were ear infections, 10 of which occurred shortly after exposure to Danish coastal seawater. The disease was mild in most of the patients and all recovered. Most of the patients showed predisposing conditions such as chronic otitis media, perforation of the tympanic membrane or ulcus cruris. The organisms were isolated in minor numbers from coastal water samples from 5 of 12 bathing areas around Funen at the end of July, but not at the end of November. This study indicates that the halophilic marine vibrios may be pathogenic in Scandinavian areas in persons exposed to seawater.
Population analyses of susceptibility to cefuroxime and cefotaxime in penicillin-susceptible, penicillin-resistant, and methicillin-resistant strains of Staphylococcus aureus (S. aureus) and ...Staphylococcus epidermidis (S. epidermidis) were carried out. All strains were clinical isolates. Both antibiotics were shown to be more penicillinase-stable than cephalothin in studies of the penicillin-resistant strains of S. aureus, but less stable than methicillin. The studies of penicillin-resistant strains of S. epidermidis showed no differences in penicillinase-stability between cephalothin and the new cephalosporins. From the methicillin-resistant strains of S. aureus and S. epidermidis it was possible to select highly-resistant mutants against both antibiotics with a frequency of c. 10(-5) although MIC determinations had shown the strains to be susceptible.
A total of 965 strains of Micrococcaceae isolated from 200 patients, personnel, and air in a department of cardiac surgery were classified by means of Baird-Parker's scheme. The majority of strains ...were identified as Staphylococcus epidermidis (S. epidermidis) biotype 1, but S. epidermidis biotype 4 accounted for c. 25% of isolates from patients post-operatively. Pre-operative isolates were generally sensitive to most antibiotics tested while post-operative strains of coagulase-negative Micrococcaceae from patients and isolates from personnel and air were frequently multiply-resistant. Strains of Staphylococcus aureus (S. aureus) were sensitive or resistant only to penicillin. More patients were colonized with coagulase-negative Micrococcaceae after operation than at admission to the hospital (p less than 0.001), while the frequency of S. aureus carriers was the same before and after operation and equal to the frequency found earlier. The frequency of S. aureus carriers among the personnel, however, was lower than reported earlier (10%). Multiply-resistant strains of S. epidermidis seem to have replaced resistant strains of S. aureus as the predominant hospital saprophyte among Micrococcaceae.
Aufgrund des Vorkommens von humanen Pasteurella aerogenes-Faellen nach Schweinebissverletzungen in Daenemark wurden 6 Isolate von Menschen und 15 Isolate aus Schweinen, Ferkeln, Hunden, Kaninchen aus ...den Laendern der USA, Kanada, Tschechoslowakei, Frankreich, Belgien und Daenemark miteinander verglichen. Durch Ribotypisierung wurden 19 Staemme auf einem Aehnlichkeitsniveau von 81 % oder mehr verbunden. Phaenotypische Tests und Ribotypisierung zeigten, dass 2 Staemme nicht der Spezies P. aerogenes angehoerten. P. aerogenes Isolate bilden eine abgegrenzte Gruppe und konnten nicht auf der Grundlage der Wirtsspezies oder der geographischen Herkunft unterteilt werden.
