Several investigators have proposed that carcinoembryonic antigen (CEA), an immunogenic antigen expressed by colon carcinoma, may also be expressed by human melanoma. Because sialyl Lewisx (sLex), ...the carbohydrate moiety of CEA, has been identified in melanoma, we compared CEA and sLex levels in colon carcinoma cells and melanoma cells.
CEA levels were assessed for expression on the cell surface and in cell lysates of cutaneous melanoma cell lines by two different kinds of ELISA, and by Western blot analysis of immunoprecipitated CEA using monoclonal antibodies (Mabs) T84-66 and COL-1, which have defined specificities for CEA. Colon carcinoma cells and purified CEA were positive controls.
Both Mabs reacted strongly with cell surface and cell lysates of colon cancer. Mab T84-66 reacted well with cell surface but not cell lysates of melanoma. COL-1 reacted poorly with cell surface but its binding increased with the density of melanoma cell lysates. Both Mabs intensely stained the blots of purified CEA and colon carcinoma lysates immunoprecipitated with the respective Mabs, but failed to stain the immunoprecipitates of melanoma cell lysates. Both Mabs bound to lysates immunoprecipitated with anti-sLex Mab in colon carcinoma, but not in melanoma. Cell-surface expression of CEA and sLex was significantly correlated (r2: 0.88) in colon cancer cells but not in melanoma.
Our results confirm the presence of CEA in colon carcinoma but not in human cutaneous melanoma cell lines.
We present the confirmation and characterisation of GJ 3473 b (G 50--16, TOI-488.01), a hot Earth-sized planet orbiting an M4 dwarf star, whose transiting signal ...(\(P=1.1980035\pm0.0000018\mathrm{\,d}\)) was first detected by the Transiting Exoplanet Survey Satellite (TESS). Through a joint modelling of follow-up radial velocity observations with CARMENES, IRD, and HARPS together with extensive ground-based photometric follow-up observations with LCOGT, MuSCAT, and MuSCAT2, we determined a precise planetary mass, \(M_b = 1.86\pm0.30\,\mathrm{M_\oplus},\) and radius, \(R_b = {1.264\pm0.050}\,\mathrm{R_\oplus}\). Additionally, we report the discovery of a second, temperate, non-transiting planet in the system, GJ 3473 c, which has a minimum mass, \(M_c \sin{i} = {7.41\pm0.91}\,\mathrm{M_\oplus,}\) and orbital period, \(P_c={15.509\pm0.033}\,\mathrm{d}\). The inner planet of the system, GJ 3473 b, is one of the hottest transiting Earth-sized planets known thus far, accompanied by a dynamical mass measurement, which makes it a particularly attractive target for thermal emission spectroscopy.
Measurements of neutron capture cross-sections for ADS-related studies Milazzo, P.M.; Aerts, G.; Berthoumieux, E. ...
Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms,
2004, 2004-1-00, Letnik:
213
Journal Article, Conference Proceeding
Recenzirano
Odprti dostop
Capture cross-sections on several isotopes relevant to accelerator driven systems for energy production and nuclear waste transmutation, and to stellar nucleosynthesis can be studied at the ...innovative neutron time of flight facility (n_TOF) at CERN. The experimental apparatus is based on a low-mass Si-based flux monitor and a set of C
6D
6 liquid scintillator detectors. The accurate reconstruction of the cross-sections relies on the pulse height weighting function technique. The set-up used in the measurements is here described. The first results on reference isotopes, Au, Ag and Fe, used to verify the accuracy of the method are presented.
The prognosis of autosomal recessive polycystic kidney disease is known to correlate with genotype. The presence of two truncating mutations in the PKHD1 gene encoding the fibrocystin protein is ...associated with neonatal death while patients who survive have at least one missense mutation. To determine relationships between genotype and renal and hepatic abnormalities we correlated the severity of renal and hepatic histological lesions to the type of PKHD1 mutations in 54 fetuses (medical pregnancy termination) and 20 neonates who died shortly after birth. Within this cohort, 55.5% of the mutations truncated fibrocystin. The severity of cortical collecting duct dilatations, cortical tubule and glomerular lesions, and renal cortical and hepatic portal fibrosis increased with gestational age. Severe genotypes, defined by two truncating mutations, were more frequent in patients of less than 30 weeks gestation compared to older fetuses and neonates. When adjusted to gestational age, the extension of collecting duct dilatation into the cortex and cortical tubule lesions, but not portal fibrosis, was more prevalent in patients with severe than in those with a non-severe genotype. Our results show the presence of two truncating mutations of the PKHD1 gene is associated with the most severe renal forms of prenatally detected autosomal recessive polycystic kidney disease. Their absence, however, does not guarantee survival to the neonatal period.
The underlying causes and rationale for treatment of recurrent abortion are not entirely clear. The Euro-Team early pregnancy protocol was developed as a diagnostic work-up based on the evaluation of ...risk factors. Possibilities for therapy can be based only on the expectancy that elimination of some risk factors may improve the prognosis.
The commissioning phase Bortoletto, F; Benetti, S; Bonanno, G ...
arXiv.org,
02/2023
Paper, Journal Article
Odprti dostop
In May 1997 a consistent part of the services and structures committed to the industry had already been released to the commissioning group. The telescope itself was, with the exception of the ...Nasmyth derotators, motors and all the optics groups, basically ready in its mechanical parts to accept the integration of all services and control equipment. Also the verification of the cabling (interlocks, data-nets, power and controls) already mounted was started in the same period. Starting from June 1998 (telescope first-light date) the telescope went gradually in use, several nights per week, in order to test and tune the tracking and pointing system, the optics and the first derotator system (Nasmyth A station). At the end of the commissioning period and with the first scientific instruments mounted (April 1999) also the first routinely observations started. In this moment the telescope is doing astronomy 80% of time and the complete first-light instrumentation is mounted.
We report the discovery, mass, and radius determination of TOI-1801 b, a temperate mini-Neptune around a young M dwarf. TOI-1801 b was observed in TESS sectors 22 and 49, and the alert that this was ...a TESS planet candidate with a period of 21.3 days went out in April 2020. However, ground-based follow-up observations, including seeing-limited photometry in and outside transit together with precise radial velocity (RV) measurements with CARMENES and HIRES revealed that the true period of the planet is 10.6 days. These observations also allowed us to retrieve a mass of 5.74 \(\pm\) 1.46 \(M_\oplus\), which together with a radius of 2.08 \(\pm\) 0.12 \(R_\oplus\), means that TOI-1801 b is most probably composed of water and rock, with an upper limit of 2\% by mass of H\(_{2}\) in its atmosphere. The stellar rotation period of 16 days is readily detectable in our RV time series and in the ground-based photometry. We derived a likely age of 600--800 Myr for the parent star TOI-1801, which means that TOI-1801 b is the least massive young mini-Neptune with precise mass and radius determinations. Our results suggest that if TOI-1801 b had a larger atmosphere in the past, it must have been removed by some evolutionary mechanism on timescales shorter than 1 Gyr.
A complete accounting of nearby objects -- from the highest-mass white dwarf
progenitors down to low-mass brown dwarfs -- is now possible, thanks to an
almost complete set of trigonometric parallax ...determinations from Gaia,
ground-based surveys, and Spitzer follow-up. We create a census of objects
within a Sun-centered sphere of 20-pc radius and check published literature to
decompose each binary or higher-order system into its separate components. The
result is a volume-limited census of $\sim$3,600 individual star formation
products useful in measuring the initial mass function across the stellar ($<8
M_\odot$) and substellar ($\gtrsim 5 M_{Jup}$) regimes. Comparing our resulting
initial mass function to previous measurements shows good agreement above
0.8$M_\odot$ and a divergence at lower masses. Our 20-pc space densities are
best fit with a quadripartite power law, $\xi(M) = dN/dM \propto M^{-\alpha}$
with long-established values of $\alpha = 2.3$ at high masses ($0.55 < M < 8.00
M_\odot$) and $\alpha = 1.3$ at intermediate masses ($0.22 < M < 0.55
M_\odot$), but at lower masses we find $\alpha = 0.25$ for $0.05 < M <0.22
M_\odot$ and $\alpha = 0.6$ for $0.01 < M < 0.05 M_\odot$. This implies that
the rate of production as a function of decreasing mass diminishes in the
low-mass star/high-mass brown dwarf regime before increasing again in the
low-mass brown dwarf regime. Correcting for completeness, we find a star to
brown dwarf number ratio of, currently, 4:1, and an average mass per object of
0.41 $M_\odot$.
The excited states of unstable \(^{20}\)O were investigated via \(\gamma\)-ray spectroscopy following the \(^{19}\)O\((d,p)^{20}\)O reaction at 8 \(A\)MeV. By exploiting the Doppler Shift Attenuation ...Method, the lifetime of the 2\(^+_2\) and 3\(^+_1\) states were firmly established. From the \(\gamma\)-ray branching and E2/M1 mixing ratios for transitions deexciting the 2\(^+_2\) and 3\(^+_1\) states, the B(E2) and B(M1) were determined. Various chiral effective field theory Hamiltonians, describing the nuclear properties beyond ground states, along with a standard USDB interaction, were compared with the experimentally obtained data. Such a comparison for a large set of \(\gamma\)-ray transition probabilities with the valence space in medium similarity renormalization group ab-initio calculations was performed for the first time in a nucleus far from stability. It was shown that the ab-initio approaches using chiral EFT forces are challenged by detailed high-precision spectroscopic properties of nuclei. The reduced transition probabilities were found to be a very constraining test of the performance of the ab-initio models.
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