Sirtuin 3 (SIRT3) is a potential therapeutic target for cardiovascular, metabolic, and other aging-related diseases. In this study, we investigated the role of SIRT3 in diabetic cardiomyopathy (DCM). ...Mice were injected with streptozotocin (STZ, 60 mg/kg, ip) to induce diabetes mellitus. Our proteomics analysis revealed that SIRT3 expression in the myocardium of diabetic mice was lower than that of control mice, as subsequently confirmed by real-time PCR and Western blotting. To explore the role of SIRT3 in DCM, SIRT3-knockout mice and 129S1/SvImJ wild-type mice were injected with STZ. We found that diabetic mice with SIRT3 deficiency exhibited aggravated cardiac dysfunction, increased lactate dehydrogenase (LDH) level in the serum, decreased adenosine triphosphate (ATP) level in the myocardium, exacerbated myocardial injury, and promoted myocardial reactive oxygen species (ROS) accumulation. Neonatal rat cardiomyocytes were transfected with SIRT3 siRNA, then exposed to high glucose (HG, 25.5 mM). We found that downregulation of SIRT3 further increased LDH release, decreased ATP level, suppressed the mitochondrial membrane potential, and elevated oxidative stress in HG-treated cardiomyocytes. SIRT3 deficiency further raised expression of necroptosis-related proteins including receptor-interacting protein kinase 1 (RIPK1), RIPK3, and cleaved caspase 3, and upregulated the expression of inflammation-related proteins including NLR family pyrin domain-containing protein 3 (NLRP3), caspase 1 p20, and interleukin-1β both in vitro and in vivo. Collectively, SIRT3 deficiency aggravated hyperglycemia-induced mitochondrial damage, increased ROS accumulation, promoted necroptosis, possibly activated the NLRP3 inflammasome, and ultimately exacerbated DCM in the mice. These results suggest that SIRT3 can be a molecular intervention target for the prevention and treatment of DCM.
In recent years there has been an exponential growth in the number of publications related to theory and applications of Data Envelopment Analysis (DEA). Charnes, Cooper, and Rhodes (1978) introduced ...DEA as a tool for measuring efficiency and productivity of decision making units. DEA has immediately been recognized as a modern tool for performance measurement. Since then, a large and considerable amount of articles has been appeared, including significant breakthroughs in theory and a great portion of works on DEA applications, both public and private sectors, to assess the efficiency and productivity of their activities. Although there have been several bibliographic collections reported, a comprehensive analysis and listing of DEA-related articles covering its first four decades of history is still missing. This paper, thus, aims to report an extensive listing of DEA-related articles including theory and methodology developments and "real" applications in diversified scenarios from 1978 to end of 2016. Some summary statistics of the publications' growth, the most utilized academic journals, authorship analysis, as well as keywords analysis are also provided.
DNA demethylation can occur passively by “dilution” of methylation marks by DNA replication, or actively and independently of DNA replication. Direct conversion of 5‐methylcytosine (5mC) to cytosine ...(C), as originally proposed, does not occur. Instead, active DNA methylation involves oxidation of the methylated base by ten‐eleven translocations (TETs), or deamination of the methylated or a nearby base by activation induced deaminase (AID). The modified nucleotide, possibly together with surrounding nucleotides, is then replaced by the BER pathway. Recent data clarify the roles and the regulation of well‐known enzymes in this process. They identify base excision repair (BER) glycosylases that may cooperate with or replace thymine DNA glycosylase (TDG) in the base excision step, and suggest possible involvement of DNA damage repair pathways other than BER in active DNA demethylation. Here, we review these new developments.
DNA methylation is not forever. It can be lost passively during DNA replication or removed actively. Active erase of methylation begins with modification of the methylated or a nearby nucleotide by AID‐mediated deamination or TET‐mediated oxidation, and ends with replacement of the modified nucleotide by DNA damage repair pathways.
Abstract We propose to utilize two parity-protected qubits which are built based on superconductor/topological-insulator/superconductor (SC/TI/SC) Josephson junction to implement a parity-spin ...superconducting qubit. The SC/TI/SC Josephson junctions have identical Josephson potential, which is robust against fabrication variations and guarantees the reliable cos 2 ϕ energy-phase relation for implementing a parity-protected qubit. By viewing the even and odd parity ground states of a single parity-protected qubit as spin- 1 2 states, we construct the logic qubit states using the total parity odd subspace of two parity-protected qubits, refereed to parity-spin qubit. This parity-spin qubit exhibits robustness against charge noise, similar to a singlet-triplet (S-T) qubit’s immunity to global magnetic field fluctuations. Meanwhile, the flux noise cannot directly couple two states with the same total parity and is significantly suppressed. Benefiting from the simultaneous protection from charge and flux noise, we demonstrate an enhancement of both T 1 and T 2 coherence times. Our work presents a TI-based approach to engineer symmetry-protected superconducting qubits.
A biomimetic (titin protein molecular structure) strategy is reported for preparing transparent and healable elastomers featuring supertoughness (345 MJ m−3) and high tensile strength (44 MPa) after ...self‐healing enabled by hierarchical (single, double, and quadruple) hydrogen‐bonding moieties in the polymer backbone. The rigid domain containing hierarchical H‐bonds formed with urethane, urea, and 2‐ureido‐41H‐pyrimidinone groups leads to a durable network structure that has enhanced mechanical properties and is also dynamic for rapid self‐healing. Healable polymers with hierarchical hydrogen‐bonding interactions show excellent recoverability and high energy dissipation owing to the durable interaction between polymer chains. This biomimetic strategy of using hierarchical hydrogen bonds as building blocks is an alternative approach for obtaining dynamic, strong, yet smart self‐healing polymers for heavy‐duty protection materials and wearable electronics.
Supertough self‐healing elastomers were constructed by a biomimetic synthetic strategy and control of the nanophase structure with hierarchical (single, double, quadruple) hydrogen‐bonding motifs. The successive dynamic interaction of the H‐bonds and increased chain mobility contribute to the self‐healing and supertough mechanical properties. The toughness after self‐healing reached 345 MJ m−3.
It is still a great challenge to achieve high selectivity of CH4 in CO2 electroreduction reactions (CO2RR) because of the similar reduction potentials of possible products and the sluggish kinetics ...for CO2 activation. Stabilizing key reaction intermediates by single type of active sites supported on porous conductive material is crucial to achieve high selectivity for single product such as CH4. Here, Cu2O(111) quantum dots with an average size of 3.5 nm are in situ synthesized on a porous conductive copper‐based metal–organic framework (CuHHTP), exhibiting high selectivity of 73 % towards CH4 with partial current density of 10.8 mA cm−2 at −1.4 V vs. RHE (reversible hydrogen electrode) in CO2RR. Operando infrared spectroscopy and DFT calculations reveal that the key intermediates (such as *CH2O and *OCH3) involved in the pathway of CH4 formation are stabilized by the single active Cu2O(111) and hydrogen bonding, thus generating CH4 instead of CO.
Cu2O(111) single‐type sites on a conductive metal–organic framework are successfully prepared by an in situ electrochemical method. The cooperative effect between the single active Cu2O(111) and hydrogen bonding contributes to the high selectivity of 73 % towards CH4 with large current density in CO2 electroreduction reduction for the obtained Cu2O(111)@CuHHTP.
We show that the incoherent elastic scattering of partons, as present in a multi-phase transport model (AMPT), with a modest parton–parton cross-section of σ=1.5–3 mb, naturally explains the ...long-range two-particle azimuthal correlation as observed in proton–proton and proton–nucleus collisions at the Large Hadron Collider.
The electrocatalytic conversion of CO2 into value‐added chemicals is a promising approach to realize a carbon‐energy balance. However, low current density still limits the application of the CO2 ...electroreduction reaction (CO2RR). Metal–organic frameworks (MOFs) are one class of promising alternatives for the CO2RR due to their periodically arranged isolated metal active sites. However, the poor conductivity of traditional MOFs usually results in a low current density in CO2RR. We have prepared conductive two‐dimensional (2D) phthalocyanine‐based MOF (NiPc‐NiO4) nanosheets linked by nickel‐catecholate, which can be employed as highly efficient electrocatalysts for the CO2RR to CO. The obtained NiPc‐NiO4 has a good conductivity and exhibited a very high selectivity of 98.4 % toward CO production and a large CO partial current density of 34.5 mA cm−2, outperforming the reported MOF catalysts. This work highlights the potential of conductive crystalline frameworks in electrocatalysis.
Nickel phthalocyanine molecules as active sites were installed into nickel‐catecholate‐linked 2D conductive metal–organic framework nanosheets for efficient CO2 electroreduction with nearly 100 % CO selectivity.
Balancing Immunity and Yield in Crop Plants Ning, Yuese; Liu, Wende; Wang, Guo-Liang
Trends in plant science,
December 2017, 2017-12-00, 20171201, Letnik:
22, Številka:
12
Journal Article
Recenzirano
Crop diseases cause enormous yield losses and threaten global food security. The use of highly resistant cultivars can effectively control plant diseases, but in crops, genetic immunity to disease ...often comes with an unintended reduction in growth and yield. Here, we review recent advances in understanding how nucleotide-binding domain, leucine-rich repeat (NLR) receptors and cell wall-associated kinase (WAK) proteins function in balancing immunity and yield. We also discuss the role of plant hormones and transcription factors in regulating the trade-offs between plant growth and immunity. Finally, we describe how a novel mechanism of translational control of defense proteins can enhance immunity without the reduction in fitness.
High yield and immunity to pathogens are important objectives in plant breeding. However, plant growth and immunity pathways are intertwined and usually antagonistic.
Hormones are important for plant growth; however, activation of immunity redirects and initiates hormone signaling that can impair plant growth.
Transcription factors act as molecular integrators to regulate the trade-offs between immunity and growth. NLR and WAK immune receptors play dual roles in immunity and yield.
Pathogen-inducible translational control strategies can enhance plant immunity without fitness costs.
New breeding strategies should be developed to enhance immunity without sacrificing fitness and yield.
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