The SLX4 tumor suppressor is a scaffold that plays a pivotal role in several aspects of genome protection, including homologous recombination, interstrand DNA crosslink repair and the maintenance of ...common fragile sites and telomeres. Here, we unravel an unexpected direct interaction between SLX4 and the DNA helicase RTEL1, which, until now, were viewed as having independent and antagonistic functions. We identify cancer and Hoyeraal-Hreidarsson syndrome-associated mutations in SLX4 and RTEL1, respectively, that abolish SLX4-RTEL1 complex formation. We show that both proteins are recruited to nascent DNA, tightly co-localize with active RNA pol II, and that SLX4, in complex with RTEL1, promotes FANCD2/RNA pol II co-localization. Importantly, disrupting the SLX4-RTEL1 interaction leads to DNA replication defects in unstressed cells, which are rescued by inhibiting transcription. Our data demonstrate that SLX4 and RTEL1 interact to prevent replication-transcription conflicts and provide evidence that this is independent of the nuclease scaffold function of SLX4.
Reliably determining trunk volume of a growing tree and accurately measuring changing diameters along the trunk at different heights are important data to foresters world-wide. Existing methods for ...determining trunk diameter of a growing tree assume the horizontal cross-section is a circle. However, to an observer standing beside a tree and looking upwards along the trunk, the imaginary cross-section of the trunk does not look like a circle but appears elliptical. As the observer stands closer to the tree and the higher the established point where the diameter of the trunk is measured, the elliptical shape of the cross-section becomes more pronounced. Conversely, the smaller the tree and the farther the observer stands from the tree, the imaginary cross-section of the trunk becomes more circular. In this paper we describe a method that makes it possible to accurately determine the diameter of a trunk regardless of the above two distance factors. Using the mathematical parameters of an ellipse, the objective of this study was to provide an accurate method to calculate the diameter at any height on the trunk.
Fundamental thermodynamic properties of the synthetic analog of the famatinite mineral - Cu3SbS4 were studied on the basis of electromotive force (EMF) measurements. The EMF of the concentration ...chains relative to the Cu electrode with a solid electrolyte was measured for the alloys from the Cu3SbS4 + Sb2S3 + S phase region at 300-380K temperature interval. Based on measurement data, the relative partial thermodynamic functions of copper in alloys, the standard thermodynamic functions of formation, as well as, the standard entropy of the Cu3SbS4 ternary compound were calculated for the first time.
Use of the two-phase extracting system ionic liquid (IL)—supercritical CO
2
for selective purification of medium-viscosity oil fractions from a mixture of low-paraffinous Azerbaijan crude oils ...results in a three- to sixfold reduction of selective solvent (IL) consumption. The use of such a two-phase system allows one to decrease the temperature (from 80 to 31°C) and the duration of the process.
The antihypertensive effects and tolerance of a fixed low-dose combination of the angiotensin-converting enzyme (ACE) inhibitor perindopril (2 mg) and the diuretic indapamide (0.625 mg) were studied ...in patients with mild and moderate arterial hypertension. The patients' mean age was 51.35 +/- 5.02 years. The antihypertensive effects were evaluated from the results of daily blood pressure monitoring following 12 weeks of therapy. It was found that low-dose combination of perindopril and the indapamide exerted a multidirectional corrective effect on the daily blood pressure (BP): it provided a significant optimal BP lowering during a day on a long-term basis and significantly diminished the baseline increased systolic and diastolic BP variability during a day.
Alloys of the system Bi-Te obtained by long-term thermal annealing of samples quenched from 1000 K were studied by differential thermal analysis, x-ray phase diffraction, and scanning electron ...microscopy methods, as well as by electromotive forces measurements (EMF) of concentration cells relative to a bismuth electrode. A new refined version of the phase diagram which reflects the compounds Bi
2
Te
3
, Bi
4
Te
5
, Bi
8
Te
9
, BiTe, Bi
4
Te
3
, Bi
2
Te, and Bi
7
Te
3
with practically constant compositions was constructed. It was found that all of these compounds, except for Bi
2
Te
3
, melt with decomposition by peritectic reactions. Based on powder diffraction patterns, the types and parameters of the crystal lattices of these compounds were determined. The EMF measurements were used to determine the partial molar functions of bismuth in the alloys and the standard integral thermodynamic functions of bismuth tellurides.
The Bi-Se system was studied by differential thermal analysis, powder X-ray diffraction, scanning electron microscopy, as well as by electromotive forces (EMF) measurements of electrochemical cells ...(−) Bi (solid) │ionic liquid + Bi
3+
│ Bi-Se (solid) ( +). A new refined version of the phase diagram reflecting the compounds Bi
2
Se
3
, Bi
3
Se
4
, Bi
8
Se
9
, BiSe, Bi
8
Se
7
, Bi
4
Se
3
, and Bi
3
Se
2
with almost constant composition was constructed. It was established that the first compound melts congruently, and all the rest melt with peritectic reaction. The types and parameters of the crystal lattices of the above-mentioned compounds were determined based on the powder diffraction patterns. From the EMF measurements, the partial molar functions of bismuth in alloys and the standard integral thermodynamic functions of bismuth selenides were calculated. Comparative analysis of the obtained results with the literature data is carried out.
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