Although serum bile acid concentrations are approximately 10 µM in healthy subjects, the crosstalk between the biliary system and vascular repair has never been investigated. In this study, ...tauroursodeoxycholic acid (TUDCA) induced dissociation of CD34(+) hematopoietic stem cells (HSCs) from stromal cells by reducing adhesion molecule expression. TUDCA increased CD34(+) /Sca1(+) progenitors in mice peripheral blood (PB), and CD34(+) , CD31(+) , and c-kit(+) progenitors in human PB. In addition, TUDCA increased differentiation of CD34(+) HSCs into EPC lineage cells via Akt activation. EPC invasion was increased by TUDCA, which was mediated by fibroblast activating protein via Akt activation. Interestingly, TUDCA induced integration of EPCs into human aortic endothelial cells (HAECs) by increasing adhesion molecule expression. In the mouse hind limb ischemia model, TUDCA promoted blood perfusion by enhancing angiogenesis through recruitment of Flk-1(+) /CD34(+) and Sca-1(+) /c-kit(+) progenitors into damaged tissue. In GFP(+) bone marrow-transplanted hind limb ischemia, TUDCA induced recruitment of GFP(+) /c-kit(+) progenitors to the ischemic area, resulting in an increased blood perfusion ratio. Histological analysis suggested that GFP(+) progenitors mobilized from bone marrow, integrated into blood vessels, and differentiated into VEGFR(+) cells. In addition, TUDCA decreased cellular senescence by reducing levels of p53, p21, and reactive oxygen species and increased nitric oxide. Transplantation of TUDCA-primed senescent EPCs in hind limb ischemia significantly improved blood vessel regeneration, as compared with senescent EPCs. Our results suggested that TUDCA promoted neovascularization by enhancing the mobilization of stem/progenitor cells from bone marrow, their differentiation into EPCs, and their integration with preexisting endothelial cells.
A duo-binary signaling has been applied to both transmitter and receiver for high-speed low-power DRAM interface. The transmitter consists of a half-rate voltage-mode time-interleaved mixing ...duo-binary driver and a 2-tap feed-forward equalizer. The voltage-mode driver is used in this brief, because it generates more linear output voltage than the current-mode driver for the duo-binary signaling at low supply voltage. A time-based receiver is used for high-speed operation at low supply voltage. A 1-tap look-ahead decision-feedback equalizer scheme was applied to the time-based receiver for the duo-binary decoding operation. The test chip fabricated in a 28 nm low-power CMOS process gives the energy efficiency of 0.41 pJ/b at 12 Gb/s with a 25 cm-long FR-4 micro-strip line and the supply voltage of 0.75V.
Rab escort protein 1 (REP1) is a component of Rab geranyl-geranyl transferase 2 complex. Mutations in REP1 cause a disease called choroideremia (CHM), which is an X-linked eye disease. Although it is ...postulated that REP1 has functions in cell survival or death of various tissues in addition to the eye, how REP1 functions in normal and cancer cells remains to be elucidated. Here, we demonstrated that REP1 is required for the survival of intestinal cells in addition to eyes or a variety of cells in zebrafish, and also has important roles in tumorigenesis. Notably, REP1 is highly expressed in colon cancer tissues and cell lines, and silencing of REP1 sensitizes colon cancer cells to serum starvation- and 5-FU-induced apoptosis. In an effort to elucidate the molecular mechanisms underlying REP1-mediated cell survival under those stress conditions, we identified FOXO3 as a binding partner of REP1 using a yeast two-hybrid (Y2H) assay system, and we demonstrated that REP1 blocked the nuclear trans-localization of FOXO3 through physically interacting with FOXO3, thereby suppressing FOXO3-mediated apoptosis. Importantly, the inhibition of REP1 combined with 5-FU treatment could lead to significant retarded tumor growth in a xenograft tumor model of human cancer cells. Thus, our results suggest that REP1 could be a new therapeutic target in combination treatment for colon cancer patients.
is a plant grown in Korea and Japan with a particular natural compound, hydrangenol.
has been researched for its anti-fungal properties, and ability to attenuate allergies and promote muscle growth. ...Its ability to reduce skin dryness is poorly understood. For that reason, we investigated whether
hot water extracts (Hs-WE) can moisturize keratinocytes. In clinical studies (Approval Code: GIRB-21929-NY and approval Date: 5 October 2021), skin wrinkles and skin moisturizing levels were improved in subjects applying 0.5% Hs-WE compared to the placebo group. We confirmed the components of Hs-WE from the LC/MS-MS analysis. Hs-WE and hydrangenol did not show cytotoxicity in HaCaT cells at all concentrations. Cell growth was also promoted by Hs-WE (5-20 µg/mL) and hydrangenol (15-60 µM) in a wound healing assay. Skin moisturizing factors were upregulated by the presence of Hs-WE or hydrangenol, and the hyaluronidases (HYAL) were inhibited at the mRNA level. Meanwhile, COL1A1 was increased by the presence of Hs-WE or hydrangenol. MAPK, AP-1, and Akt/PI3k signaling proteins, which are associated with cell proliferation and moisturizing factors, were increased by the administration of Hs-WE and hydrangenol. Has-1, 2, and 3 levels were enhanced via JNK when using the inhibitors of MAPK proteins and Hs-WE and hydrangenol, respectively. Taken together, Hs-WE could be used as cosmeceutical materials for improving skin conditions.
Recently, wind power plants that generate wind energy with electricity are attracting a lot of attention thanks to their smaller installation area and cheaper power generation costs. In wind power ...generation, it is important to predict the amount of generated electricity because the power system would be unstable due to uncertainty in supply. However, it is difficult to accurately predict the amount of wind power generation because the power varies due to several causes, such as wind speed, wind direction, temperature, etc. In this study, we deal with a mid-term (one day ahead) wind power forecasting problem with a data-driven approach. In particular, it is intended to solve the problem of a newly completed wind power generator that makes it very difficult to predict the amount of electricity generated due to the lack of data on past power generation. To this end, a deep learning based transfer learning model was proposed and compared with other models, such as a deep learning model without transfer learning and Light Gradient Boosting Machine (LGBM). As per the experimental results, when the proposed transfer learning model was applied to a similar wind power complex in the same region, it was confirmed that the low predictive performance of the newly constructed generator could be supplemented.
There are two types of maintenance policies for equipment: breakdown maintenance and preventive maintenance. In the case of applying preventive maintenance, the maintenance is carried out based on ...time or the condition of the equipment. However, with the development of Information and Communications Technologies (ICT) and the Internet of Things (IoT) technology, the data collected from equipment has rapidly increased and the use of Condition-Based Maintenance (CBM) to perform appropriate maintenance based on the condition of the equipment is increasing. In this study, based on gathered sensor data, we introduce an approach to diagnosing the condition of the equipment by extracting specific data features related to the types of failures that occur with equipment. To this end, we used the K-means clustering method, support vector machine (SVM) classifier, and Pattern Frequency–Inverse Failure mode Frequency (PF–IFF) method with the Term Frequency–Inverse Document Frequency (TF–IDF) method. As a case study, we applied the proposed approach to a centrifugal pump and carried out computational experiments for assessing the performance and validity of the proposed approach.
In large systems, such as power plants or petrochemical plants, various equipment (e.g., compressors, pumps, turbines, etc.) are typically deployed. Each piece of equipment operates under generally ...harsh operating conditions, depending on its purpose, and operates with a probability of failure. Therefore, several sensors are attached to monitor the status of each piece of equipment to observe its conditions; however, there are many limitations in monitoring equipment using thresholds such as maximum and minimum values of data. Therefore, this study introduces a technology that can diagnose fault conditions by analyzing several sensor data obtained from plant operation information systems. The equipment for the case study was a main air blower (MAB), an important cooling equipment in the plant process. The driving sensor data were analyzed for approximately three years, measured at the plant. The fault history of the actual process was also analyzed. Due to the large number of sensors installed in the MAB system, a dimension reduction method was applied with the principal component analysis (PCA) method when analyzing collected sensor data. For application to PCA, the collected sensor data were analyzed according to the statistical analysis method and data features were extracted. Then, the features were labeled and classified according to normal and fault operating conditions. The analyzed features were converted with a diagnosis model, by dimensional reduction, applying the PCA method and a classification algorithm. Finally, to validate the diagnosis model, the actual failure signal that occurred in the plant was applied to the suggested method. As a result, the results from diagnosing signs of failure were confirmed even before the failure occurred. This paper explains the case study of fault diagnosis for MAB equipment with the suggested method and its results.
A time-based (TB) receiver (RX) with a 2-tap TB decision feedback equalizer (DFE) is proposed for mobile DRAM interface. The TB RX consists of a voltage-to-time converter (VTC), a TB DFE, and a time ...comparator. The VTC converts the RX input voltage to a time difference between two VTC outputs by using the difference in clock-to-Q delays between two latches with different input offset voltages. The TB DFE inserts an additional delay to one of the two VTC outputs and bypasses the other VTC output to increase the time opening. The time comparator makes a decision with the first arriving edge of the two outputs of the TB DFE. While the feedback loop delay must be less than 1 UI for proper operation in the conventional voltage-based DFE, the TB DFE allows the feedback loop delay up to 1.43 UI in this paper. A transmitter (TX) transmits a single-ended signal of 200-mV swing by using an n-over-n voltage-mode driver. The transceiver in a 65-nm CMOS process achieves a 12.5 Gb/s with a 0.8-V supply through a 15-inch FR-4 channel of 14-dB loss. The TX and RX chip consume 4.3 and 3.4 mA, respectively. The energy efficiency is 0.49 pJ/b.
Sedation can resolve anxiety and fear in patients undergoing endoscopy. The use of sedatives has increased in Korea. Appropriate sedation is a state in which the patient feels subjectively ...comfortable while maintaining the airway reflex for stable spontaneous breathing. The patient should maintain a state of consciousness to the extent that he or she can cooperate with the needs of the medical staff. Despite its benefits, endoscopic sedation has been associated with cardiopulmonary complications. Such cardiopulmonary complications are usually temporary, and most patients recover without sequelae. However, these events may progress to serious complications, such as cardiovascular collapse. Therefore, it is essential to screen high-risk patients before sedation and reduce complications by meticulous monitoring. Additionally, physicians should be familiar with the management of emergencies. The first Korean clinical practice guideline for endoscopic sedation was developed based on previous worldwide guidelines for endoscopic sedation using an adaptation process. The guideline consists of nine recommendations based on a critical review of currently available data and expert consensus when the guideline was drafted. These guidelines should provide clinicians, nurses, medical school students, and policy makers with information on how to perform endoscopic sedation with minimal risk.
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