In the light of the lack of authorized COVID-19 vaccines adapted to the Omicron variant lineage, the administration of the first and second booster dose is recommended. It remains important to ...monitor the efficacy of such an approach in order to inform future preventive strategies. The present paper summarizes the research progress on the effectiveness of the first and second booster doses of COVID-19. It also discusses the potential approach in vaccination strategies that could be undertaken to maintain high levels of protection during the waves of SARS-CoV-2 infections. Although this approach can be based, with some shortcomings, on the first-generation vaccines, other vaccination strategies should be explored, including developing multiple antigen-based (multivariant-adapted) booster doses with enhanced durability of immune protection, e.g., through optimization of the half-life of generated antibodies.
Microbial degradation is an important route for removing environmental microcystins (MCs). Here, we investigated the ecological distribution of microcystin degraders (
-genotype), and the ...relationship between the substrate specificity of the microcystin degrader and the profile of microcystin congener production in the habitat. We showed that microcystin degraders were widely distributed and closely associated with
abundance in Lake Taihu, China. We characterized an indigenous degrader,
N5 in the northern Lake Taihu, and it metabolized six microcystin congeners in increasing order (RR > LR > YR > LA > LF and LW). Such a substrate-specificity pattern was congruent to the order of the dominance levels of these congeners in northern Lake Taihu. Furthermore, a meta-analysis on global microcystin degraders revealed that the substrate-specificity patterns varied geographically, but generally matched the profiles of microcystin congener production in the degrader habitats, and the indigenous degrader typically metabolized well the dominant MC congeners, but not the rare congeners in the habitat. This highlighted the phenotypic congruence between microcystin production and degradation in natural environments. We theorize that such congruence resulted from the metabolic adaptation of the indigenous degrader to the local microcystin congeners. Under the nutrient microcystin selection, the degraders might have evolved to better exploit the locally dominant congeners. This study provided the novel insight into the ecological distribution and adaptive degradation of microcystin degraders.
Summary
The biological role of the widespread mycosporine‐like amino acids (MAAs) in cyanobacteria is under debate. Here, we have constructed and characterized two mutants impaired in MAA ...biosynthesis in the bloom‐forming cyanobacterium Microcystis aeruginosa PCC 7806. We could identify shinorine as the sole MAA type of the strain, which is exclusively located in the extracellular matrix. Bioinformatic studies as wells as polymerase chain reaction screening revealed that the ability to produce MAAs is sporadically distributed within the genus. Growth experiments and reactive oxygen species quantification with wild‐type and mutant strains did not support a role of shinorine in protection against UV or other stress conditions in M. aeruginosa PCC 7806. The shinorine content per dry weight of cells as well as transcription of the mys gene cluster was not significantly elevated in response to UV‐A, UV‐B or any other stress condition tested. Remarkably, both mutants exhibited pronounced morphological changes compared with the wild type. We observed an increased accumulation and an enhanced hydrophobicity of the extracellular matrix. Our study suggests that MAAs in Microcystis play a negligible role in protection against UV radiation but might be a strain‐specific trait involved in extracellular matrix formation and cell–cell interaction.
China hosts rich shale gas resources. The Longmaxi Formation (LF) in the Sichuan Basin is one of the important regions for shale gas exploration and extraction. Here, we investigate the pore ...structure and factors influencing the gas-bearing characteristics of the pores in the shale reservoirs in the LF. We employed scanning electron microscopy (SEM), X-ray diffraction analysis (XRD), gas content testing, gas saturation testing based on nuclear magnetic resonance (NMR), overburden porosity and permeability analysis, distribution of pore size, specific surface area (SSA) analysis, and geochemical analysis. Our results show that the organic matter of the shale in the LF in the study area is highly favorable to shale gas conditions; the brittleness of the regional shale generally increases with an increase in depth. The pores in the LF mainly include organic matter-hosted pores, intergranular pores, intragranular pores, and microfissures, with mesopores dominating. Meanwhile, the gas content also increases with an increase in depth. The pore structure in the study area is affected by organic matter content and mineral components, with the development of organic matter-hosted pores. The porosity and the SSA are positively correlated with the total organic carbon (TOC), whereas clay minerals have an adverse effect on the SSA of pores in the study area. Higher TOC and vitrinite reflectance (Ro) indicate higher gas content. It can be inferred that as the content of brittle minerals increases, particles break to form storage spaces as the depth increases, causing the gas content to increase. Higher SSA and total pore volume indicate higher volume of adsorbed gas. The bound water in rocks tends to reduce the content of desorbed gas. Our results suggest high potential for shale gas extraction in this region.
Lake beach bar sandstone has been regarded as an important oil and gas reservoir in China. However, types and depositional mechanisms of the sandstone have not yet been fully studied. In this study, ...we analyzed modern beach bars in Qinghai Lake, the largest modern lake in China. The key factors controlling beach bar development in the lake were found to be wave length and height, lakeshore terrain, and material supply. Sedimentary models were established based on modern coastal dynamic theory. Beach bars were divided into breaking and surf bars. Breaking bars develop near the breaking zone where water depth is a function of wave height and length. Maximum breaking bar thickness is calculated from the difference between breaking point depth and breaking wave height. Surf bars develop in the surf zone where surf water depth equals breaking wave height. Maximum surf bar thickness is given by the breaking wave height. Wind waves play an important role in the location and construction of Qinghai Lake beach bars.
The human microbiome project has revolutionized our understanding of the interaction between commensal microbes and human health. By far, the biggest perturbation of the microbiome involves use of ...broad-spectrum antibiotics excreted in the gut. Thus, pharmacodynamics of microbiome changes in relation to drug exposure pharmacokinetics is an emerging field. However, reproducibility studies are necessary to develop the field. A simple and fast high-performance liquid chromatography-photodiode array detector (HPLC) method was validated for quantitative fecal vancomycin analysis. Reproducibility of results were tested based on sample storage time, homogeneity of antibiotic within stool, and concentration consistency after lyophilization. The HPLC method enabled the complete elution of vancomycin within ~4.2 min on the reversed-phase C18 column under the isocratic elution mode, with excellent recovery (85% to 110%) over a 4-log, quantitative range (0.4-100 μg/mL). Relative standard derivations (RSD) of intra-day and inter-day results ranged from 0.4% to 5.4%. Using sample stool aliquots of various weights consistently demonstrated similar vancomycin concentrations (mean RSD: 6%; range: 2-16%). After correcting for water concentrations, vancomycin concentrations obtained after lyophilization were similar to the concentrations obtained from the original samples (RSD less than 10%). These methodologies establish sample condition standards for a quantitative HPLC to enable vancomycin pharmacokinetic studies with the human microbiome.
Research on antibiotic effect on the gut microbiome is an emerging field with standardization of research methods needed. In this study, a simple and fast high-performance liquid chromatography method was validated for quantitative fecal vancomycin analysis. Reproducibility of results were tested to standardize storage time, homogeneity of antibiotic within stool, and concentration consistency after lyophilization. These methodologies establish sample condition standards for a quantitative HPLC to enable vancomycin pharmacokinetic studies with the human microbiome.
Vancomycin is not appreciably passaged via the colonic membrane to the gastrointestinal (GI) tract in persons with an intact gut epithelium due to its large chemical structure. However; hospitalized ...patients with diarrhea often have a disrupted GI tract. The aim of this study was to determine the frequency of detectable vancomycin concentrations in the stool of patients with antibiotic-associated diarrhea receiving IV vancomycin. This was a multicenter cohort study of hospitalized patients with stool samples collected for Clostridioides difficile testing. Leftover stool samples were collected from patients who had received at least 3 days of IV vancomycin. Fecal vancomycin was quantified by high-performance liquid chromatography. The study cohort included 33 unique patients, majority female (54.5%) aged 60 years (range 23–84). Eighteen of thirty-three patients (54.5%) tested positive for C. difficile toxins. The average duration of systemic vancomycin administration prior to stool collection was 3.5 (range 2–15) days. Three of 33 (9%) stool samples had a detectable vancomycin concentration (range 1.2–13.2 mcg/mL). These concentrations may promote the development of vancomycin-resistant Enterococcus or van mutations in C. difficile, leading to vancomycin resistance. Further studies on implications are warranted.
is the most common freshwater bloom-forming cyanobacteria. Its massive blooms not only adversely affect the functionality of aquatic ecosystems, but are also associated with the production of ...microcystins (MCs), a group of potent toxins that become a threat to public health when cell-bound MCs are significantly released from the dying
into the water column. Managing
blooms thus requires sufficient knowledge regarding both the cell death modes and the release of toxins. Recently, more and more studies have demonstrated the occurrence of programmed cell death-like (or apoptosis-like) events in laboratory and field samples of
. Apoptosis is a genetically controlled process that is essential for the development and survival of metazoa; however, it has been gradually realized to be an existing phenomenon playing important ecological roles in unicellular microorganisms. Here, we review the current progress and the existing knowledge gap regarding apoptosis-like death in
. Specifically, we focus first on the tools utilized to characterize the apoptosis-related biochemical and morphological features in
. We further outline various stressful stimuli that trigger the occurrence of apoptosis and discuss the potential mechanisms of apoptosis in
. We then propose a conceptual model to describe the functional coupling of apoptosis and MC in
. This model could be useful for understanding both roles of MC and apoptosis in this species. Lastly, we conclude the review by highlighting the current knowledge gap and considering the direction of future research. Overall, this review provides a recent update with respect to the knowledge of apoptosis in
and also offers a guide for future investigations of its ecology and survival strategies.
Grain size analysis is a common method in the study of sedimentology. For the consolidated sedimentary rocks, the traditional methods are rock slice observation and image analysis. In recent years, ...laser particle size analyzer is used widely in particle size analysis of sedimentary rock. Unlike the pretreatment of loose samples, the rock samples must be crushed, added acid to wipe out cement, and washed. However, in the step of washing, most of the fines component (less than 63 μm) in the suspended state should be inevitably lost. It will significantly affect the accuracy of particle size analysis, especially for siltstone. This paper presents a siltstone sample pretreatment method which core step is washing acid by centrifuge. Compared with traditional decantation method, the results show that the median particle size reduced 33.2 μm on average. Compared with the precipitation method which is commonly used for handling loose samples, the change of solid-liquid separation time is from 12 h to 10 min, while the average reduction of median particle size is about 15 μm. The grain size value corresponded to the cumulative volume of 10%/90% reduced 2.5 μm/20.3 μm on average. The percentage of the clay component less than 2 μm increased 2.88% on average. The fine particle (2–4 μm) and silt component (4–63 μm) increased 1.71% and 5.56% on average. Based on this method, two kinds of similar lacustrine siltstone were analyzed. They are tempestite and beach bar which are difficult to identify in the Lijin sub-depression, Dongying depression, Shengli oilfield, China. The final grain-size probability plot of tempestite is the type of “one saltation component and three suspension components”. The content of suspension components can reach to 80%–90%. The beach bar is the type of “one saltation component and two suspension components”. The content of suspension components can reach to 40%–45%. They both have the characteristics of high slope which means well sorting. But they can be distinguished based on the suspension sedimentary characteristics which were preserved by maximum degree in this kind of sample pretreatment method.
Exploring the geological factors that affect fluid flow has always been a hot topic. For tight reservoirs, the pore structure and characteristics of different lithofacies reveal the storage status of ...fluids in different reservoir environments. The size, connectivity, and distribution of fillers in different sedimentary environments have always posed a challenge in studying the microscopic heterogeneity. In this paper, six logging curves (gamma-ray, density, acoustic, compensated neutron, shallow resistivity, and deep resistivity) in two marker wells, namely, J1 and J2, of the Permian Lucaogou Formation in the Jimsar Basin are tested by using four reinforcement learning algorithms: LogitBoost, GBM, XGBoost, and KNN. The total percent correct of training well J2 is 96%, 96%, 96%, and 96%, and the total percent correct of validation well J1 is 75%, 68%, 72%, and 75%, respectively. Based on the lithofacies classification obtained by using reinforcement learning algorithm, micropores, mesopores, and macropores are comprehensively described by high-pressure mercury injection and low-pressure nitrogen gas adsorption tests. The multifractal theory servers for the quantitative characterization of the pore distribution heterogeneity regarding different lithofacies samples, and as observed, the higher probability measure area of the generalized fractal spectrum affects the heterogeneity of the local interval of mesopores and macropores of the estuary dam. In the micropore and mesopore, the heterogeneity of the evaporation lake showed a large variation due to the influence of the higher probability measure area, and in the mesopore and macropore, the heterogeneity of the evaporation lake was controlled by the lower probability measure area. According to the correlation analysis, the single-fractal dimension is well related to the multifractal parameters, and the individual fitting degree reaches up to 99%, which can serve for characterizing the pore size distribution uniformity. The combination of boosting machine learning and multifractal can help to better characterize the micro-heterogeneity under different sedimentary environments and different pore size distribution ranges, which is helpful in the exploration and development of oil fields.
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