The linear long non-coding RNA P14AS has previously been reported to be dysregulated in colon cancer, but the mechanistic role that P14AS plays in colon cancer progression has yet to be clarified. ...Accordingly, this study was developed to explore the regulatory functions of ANRIL linear transcript-P14AS in cancer.
The expression of P14AS, ANRIL, miR-23a-5p and their target genes were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. Cell supernatants of IL6 and IL8 were measured by Enzyme linked immunosorbent (ELISA) assay. Dual-luciferase reporter assays, RNA immunoprecipitation, or pull-down assays were used to confirm the target association between miR-23a-5p and P14AS or UBE2D3. Cell proliferation and chemosensitivity of NF-κB inhibitor BAY 11-7085 were evaluated by cell counting kit 8 (CCK8).
When P14AS was overexpressed in colon cancer cell lines, enhanced TNF-NF-κB signaling pathway activity was observed together with increases in IL6 and IL8 expression. The Pita, miRanda, and RNA hybrid databases revealed the ability of miR-23a-5p to interact with P14AS, while UBE2D3 was further identified as a miR-23a-5p target gene. The results of dual-luciferase reporter, RNA pull-down, and RNA immunoprecipitation experiments confirmed these direct interactions among P14AS/miR-23a-5p/UBE2D3. The degradation of IκBa mediated by UBE2D3 may contribute to enhanced NF-κB signaling in these cells. Moreover, the beneficial impact of P14AS on colon cancer cell growth was eliminated when cells were treated with miR-23a-5p inhibitors or UBE2D3 was silenced. As such, these findings strongly supported a role for the UBE2D3/IκBa/NF-κB signaling axis as a mediator of the ability of P14AS to promote colon cancer progression.
These data suggested a mechanism through which the linear ANRIL transcript P14AS can promote inflammation and colon cancer progression through the sequestration of miR-23a-5p and the modulation of NF-κB signaling activity, thus highlighting P14AS as a promising target for therapeutic intervention efforts.
Abstract
Iron phthalocyanine (FePc) is a promising non-precious catalyst for the oxygen reduction reaction (ORR). Unfortunately, FePc with plane-symmetric FeN
4
site usually exhibits an ...unsatisfactory ORR activity due to its poor O
2
adsorption and activation. Here, we report an axial Fe–O coordination induced electronic localization strategy to improve its O
2
adsorption, activation and thus the ORR performance. Theoretical calculations indicate that the Fe–O coordination evokes the electronic localization among the axial direction of O–FeN
4
sites to enhance O
2
adsorption and activation. To realize this speculation, FePc is coordinated with an oxidized carbon. Synchrotron X-ray absorption and Mössbauer spectra validate Fe–O coordination between FePc and carbon. The obtained catalyst exhibits fast kinetics for O
2
adsorption and activation with an ultralow Tafel slope of 27.5 mV dec
−1
and a remarkable half-wave potential of 0.90 V. This work offers a new strategy to regulate catalytic sites for better performance.
High-capacity Ni-rich layered oxides are promising cathode materials for secondary lithium-based battery systems. However, their structural instability detrimentally affects the battery performance ...during cell cycling. Here, we report an Al/Zr co-doped single-crystalline LiNi
Co
Mn
O
(SNCM) cathode material to circumvent the instability issue. We found that soluble Al ions are adequately incorporated in the SNCM lattice while the less soluble Zr ions are prone to aggregate in the outer SNCM surface layer. The synergistic effect of Al/Zr co-doping in SNCM lattice improve the Li-ion mobility, relief the internal strain, and suppress the Li/Ni cation mixing upon cycling at high cut-off voltage. These features improve the cathode rate capability and structural stabilization during prolonged cell cycling. In particular, the Zr-rich surface enables the formation of stable cathode-electrolyte interphase, which prevent SNCM from unwanted reactions with the non-aqueous fluorinated liquid electrolyte solution and avoid Ni dissolution. To prove the practical application of the Al/Zr co-doped SNCM, we assembled a 10.8 Ah pouch cell (using a 100 μm thick Li metal anode) capable of delivering initial specific energy of 504.5 Wh kg
at 0.1 C and 25 °C.
Designing and synthesizing efficient molecular catalysts may unlock the great challenge of controlling the CO2 reduction reaction (CO2RR) with molecular precision. Nickel phthalocyanine (NiPc) ...appears as a promising candidate for this task due to its adjustable Ni active‐site. However, the pristine NiPc suffers from poor activity and stability for CO2RR owing to the poor CO2 adsorption and activation at the bare Ni site. Here, a ligand‐tuned strategy is developed to enhance the catalytic performance and unveil the ligand effect of NiPc on CO2RR. Theoretical calculations and experimental results indicate that NiPc with electron‐donating substituents (hydroxyl or amino) can induce electronic localization at the Ni site which greatly enhances the CO2 adsorption and activation. Employing the optimal catalyst—an amino‐substituted NiPc—to convert CO2 into CO in a flow cell can achieve an ultrahigh activity and selectivity of 99.8% at current densities up to −400 mA cm−2. This work offers a novel strategy to regulate the electronic structure of active sites by ligand design and discloses the ligand‐directed catalysis of the tailored NiPc for highly efficient CO2RR.
A ligand‐tuned strategy is developed to boost the electrocatalytic reduction of CO2 and unlock the ligand‐directed molecular catalysis strategy. Nickel phthalocyanine decorated with electron‐donating substituents such as hydroxyl or amino can evoke electronic localization on the Ni site, enhancing the CO2 adsorption and activation. This promotes the catalytic reaction, which is positively associated with the electron‐donating abilities of substituents.
Domain boundaries are regarded as the effective active sites for electrochemical energy storage materials due to defects enrichment therein. However, layered double hydroxides (LDHs) tend to grow ...into single crystalline nano sheets due to their unique two-dimentional (2D) lattice structure. Previously, much efforts were made on the designing hierarchical structure to provide more exposed electroactive sites as well as accelerate the mass transfer. Herein, we demonstrate a strategy to introduce low angle grain boundary (LAGB) in the flakes of Ni/Co layered double hydroxides (NiCo-LDHs). These defect-rich nano flakes were self-assembled into hydrangea-like spheres that further constructed hollow cage structure. Both the formation of hierarchical structure and grain boundaries are interpreted with the synergistic effect of Ni
2+
/Co
2+
ratio in an “etching-growth” process. The domain boundary defect also results in the preferential formation of oxygen vacancy (Vo). Additionally, density functional theory (DFT) calculation reveals that Co substitution is a critical factor for the formation of adjacent lattice defects, which contributes to the formation of domains boundary. The fabricated battery-type Faradaic NiCo-LDH-2 electrode material exhibits significantly enhanced specific capacitance of 899 C·g
−1
at a current density of 1 A·g
−1
. NiCo-LDH-2//AC asymmetric capacitor shows a maximum energy density of 101.1 Wh·kg
−1
at the power density of 1.5 kW·kg
−1
.
Massive, easily accessible online health information empowers users to cope with health problems better. Most patients search for relevant online health information before seeing a doctor to ...alleviate information asymmetry. However, the mechanism of how online health information affects health empowerment is still unclear.
To study how online health information processing affects health empowerment.
We conducted a cross-sectional questionnaire study that included 343 samples from participants who had searched online health information before the consultation. Respondents' perceptions of online information cues, benefits, health literacy, and health empowerment were assessed.
Perceived argument quality and perceived source credibility have significant and positive effects on perceived information benefits, but only perceived argument quality has a significant effect on perceived decision-making benefits. Two types of perceived benefits, in turn, affect health empowerment. The effects of perceived argument quality on perceived informational benefits and perceived decision-making benefits on health empowerment are significantly stronger for the high health literacy group than the low health literacy group (t
=7.156, P<.001; t
=23.240, P<.001). While, the effects of perceived source credibility on perceived informational benefits and perceived informational benefits on health empowerment are significantly weaker for the high health literacy group than the low health literacy group (t
=-10.497, P<.001; t
=-6.344, P<.001). The effect of perceived argument quality on perceived informational benefits shows no significant difference between high and low health literacy groups.
In the context of online health information, perceived information benefits and perceived decision-making benefits are the antecedents of health empowerment, which in turn will be affected by perceived argument quality and perceived source credibility. Health literacy plays a moderating role in the relationship of some variables. To maximize health empowerment, online health information providers should strengthen information quality and provide differentiated information services based on users' health literacy.
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•1. Single-atom Co modified g-C3N4 prepared by one-step thermal-polymerization of cobalt phthalocyanine (CoPc) and urea.•2. Strong interaction between Co 3d electrons and C 2p ...electrons of CO2 activate C = O bonds in CO2 molecule.•3. Optimal 1%Co-CN sample exhibits higher CO yield of 94.9 umol/g/h than pure g-C3N4 0.25 umol/g/h.
Using solar energy to realize photoreduction of CO2 into valuable chemicals is a potential way to solve energy crisis and carbon cycle. Due to the extremely stable molecular configuration of CO2, activating CO2 molecule is the key and difficult step in the whole CO2 conversation process. In this work, we used density functional theory (DFT) to calculate the reaction pathways of CO2 to CO on pure g-C3N4 and single-atom cobalt (Co) modified g-C3N4. Theoretical calculation predicts that single-atom Co sites modified g-C3N4 (Co-CN) possess stronger CO2 adsorption ability and lower barrier of CO2 hydrogenation activation than pure g-C3N4. The strong interaction between Co 3d electrons and C 2p electrons of CO2 is the crucial factor to activate C = O bonds of CO2 molecule. Better CO2 adsorption and activation abilities also are proved in Co-CN by CO2 adsorption, temperature programmed desorption (TPD), and sensor tests. As a result, the optimal 1%Co-CN exhibits higher CO yield of 94.9 umol/g/h than pure g-C3N4 (0.25 umol/g/h). This work provides a new insight of the role of single-atom sites in CO2 reduction reactions.
For a product, how to select the optimal material is a crucial step in the design process. Multiple criteria decision making (MCDM) method has been successfully applied to solve various material ...selection problems. In the MCDM method, alternative materials are evaluated by processing criteria information. In order to handle the uncertainty and inaccuracy in the criteria information, this paper develops a novel MCDM method based on group generalized Pythagorean fuzzy weighted average (GGPFWA) operator. In the novel method, decision makers are divided into advisers and deciders. The advisers use Pythagorean fuzzy set (PFS) to represent the criteria information of alternative materials. PFS is a new extension of fuzzy set, which can effectively represent fuzzy and uncertain information. The deciders use group generalized parameters (GGPs) to judge the accuracy of criteria information provided by each adviser. The GGPs are expressed by Pythagorean fuzzy numbers. Then, the criteria information and the GGPs are aggregated by GGPFWA operator, and the aggregate value of each material is obtained. By processing the aggregate value, the ranking of alternative materials is determined and the optimal material is selected. The accuracy and reliability of the novel method are verified by two material selection cases.
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•This paper develops a novel multiple criteria decision making method, which can be used to accurately and reliably solve the material selection problem with uncertainty and inaccuracy in the criterion information.•This paper divides decision makers into advisers and deciders, the advisers use Pythagorean fuzzy set to represent the criteria information and the deciders use group generalized parameters to judge the accuracy of the criterion information.•This paper proposes the group generalized Pythagorean fuzzy weighted average operator to aggregate the criterion information and group generalized parameters.•This paper proposes the calculation method of criterion weight, adviser weight and decider weight.
Background
CDH3
is a glycoprotein with a single-span transmembrane domain that mediates cell-to-cell adhesion. Abnormal expression of
CDH3
is associated with a poor prognosis in patients with breast, ...thyroid, colorectal carcinomas and glioblastoma. Soluble
CDH3
in pleural effusions can be used as a marker for real-time monitoring of resistance to first- and second-generation EGFR-TKIs. The
CDH3
mechanism underlying lung adenocarcinomas (LUADs) has not been established.
Objective
This study analyzed the correlation between
CDH3
expression and lung cancer prognosis and the effect of down-regulation
CDH3
expression on the proliferation and migration of lung cancer cells.
Methods
CDH3
expression was studied using the Oncomine, TIMER, PanglaoDB, and GEPIA databases. The effect of
CDH3
on clinical prognosis was assessed with GEPIA, the PrognoScan database, and Kaplan–Meier plotter. The relationship between
CDH3
to immune infiltrating cells was explored using TIMER and TISIDB. The function of
CDH3
in lung cancer cell lines was determined by CCK-8 and wound healing assays in vitro. Furthermore, RNA sequencing was used to identify key signaling pathways and differentially-expressed genes.
Results
LUAD tissues had higher
CDH3
expression compared with normal tissues and were associated with worse overall survival in patients with LUAD.
CDH3
expression had positive associations with infiltration of CD4 + T cells, Tregs and exhausted T cells, but negative associations with infiltration of B cells in patients with LUAD. CCK-8 and wound healing assays revealed that downregulation of
CDH3
inhibited the proliferation and migration of cells. KEGG analysis revealed that the TGF-beta signaling pathways were demonstrated to be enriched pathways for genes negatively regulated by knockdown of
CDH3.
Conclusion
CDH3
expression affects proliferation and migration of lung cancer cells and might serve as a potential prognostic marker in LUAD patients.
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