Although the severity of a burn injury is often associated with the percentage of total body surface area burned (%TBSA), the thermoregulatory consequences of a given %TBSA injury do not account for ...the interactive effects of body morphology and metabolic heat production (Hprod).
Using a simulated burn injury model to mimic the detrimental effect of a 40% TBSA injury on whole-body evaporative heat dissipation, core temperature response to exercise in physiologically uncompensable conditions between morphologically disparate groups were examined at (i) an absolute Hprod (W), and (ii) a mass-specific Hprod (W·kg).
Healthy, young, nonburned individuals of small (SM, n = 11) or large (LG, n = 11) body size cycled for 60 min at 500 W or 5.3 W·kg of Hprod in 39°C and 20% relative humidity conditions. A 40% burn injury was simulated by affixing a highly absorbent, vapor-impermeable material across the torso (20% TBSA), arms (10% TBSA), and legs (10% TBSA) to impede evaporative heat loss in those regions.
Although the elevation in core temperature was greater in SM compared with LG at an Hprod of 500 W (SM, 1.69°C ± 0.26°C; LG, 1.05°C ± 0.26°C; P < 0.01), elevations in core temperature were not different at an Hprod of 5.3 W·kg between groups (SM, 0.99°C ± 0.32°C; LG, 1.05°C ± 0.26°C; P = 0.66).
These data suggest that among individuals with a 40% TBSA burn injury, a smaller body size leads to exacerbated elevations in core temperature during physical activities eliciting the same absolute Hprod (non-weight-bearing tasks) but not activities eliciting the same mass-specific Hprod (weight-bearing tasks).
Anterior cruciate ligament (ACL) reconstruction (ACLR) surgeries successfully restore anterior tibial translation but not tibial rotation. This study aimed to explore landing strategies focusing on ...the control of tibial rotation at landing when the ACL is most vulnerable. Three groups of male subjects (50 ACLRs, 26 basketball players, and 31 controls) participated in one‐leg forward hop tests for determining the tibial rotatory landing strategies adopted during the initial landing phase. The differences in knee kinematics and muscle activities between internal and external tibial rotatory (ITR, ETR) landing strategies were examined. A higher proportion of basketball players (34.6%) were found to adopt ITR strategies (controls: 6.5%), exhibiting significantly greater hopping distance and knee strength. After adjusting for hopping distance, subjects adopting ITR strategies were found to hop faster with straighter knees at foot contact and with greater ITR and less knee adduction angular displacement during the initial landing phase. However, significantly greater angular displacement in knee flexion, greater medial hamstring activities, and greater co‐contraction index of hamstrings and medial knee muscles were also found during initial landing. Our results support the importance of the recruitments of medial hamstrings or the local co‐contraction in assisting the rotatory control of the knee during initial landing for avoiding ACL injuries.
Equatorial Rossby waves (ERWs) are manifest as westward-propagating, planetary- scale waves that feature a symmetric pair of pressure and zonal wind fields about the equator. ERWs can modulate ...tropical convective activity, especially in South Asia and the Maritime Continents, and represent an important mode of intraseasonal variability additional to the Madden-Julian Oscillation. Changes in the frequency and intensity of ERWs during the recent decades were investigated based on observations of tropospheric winds and tropical convection. Spectral analyses indicated that ERWs appear to have intensified especially in the upper troposphere; this is associated with increased convective activity located off the equator. The strengthening and westward shift of the Walker circulation observed in the recent decades acted to increase the tropical vertical westerly shear and, subsequently, may contribute to the increased ERW activity. Further investigation on the dynamical process of the vertical zonal shear enhancement will improve the understanding of the changing ERW characteristics.
Glutamate transporters regulate excitatory neurotransmission and prevent glutamate-mediated excitotoxicity in the CNS. To better study the cellular and temporal dynamics of the expression of these ...transporters, we generated bacterial artificial chromosome promoter Discosoma red glutamate-aspartate transporter (GLAST) and green fluorescent protein glutamate transporter-1 (GLT-1) reporter transgenic mice. Analysis of these mice revealed a differential activation of the transporter promoters not previously appreciated. GLT-1 promoter activity in the adult CNS is almost completely restricted to astrocytes, often and unexpectedly in a nonoverlapping pattern with GLAST. Spinal cord GLT-1 promoter reporter, protein density, and physiology were 10-fold lower than in brain, suggesting a possible mechanism for regional sensitivity seen in disease. The GLAST promoter is active in both radial glia and many astrocytes in the developing CNS but is downregulated in most astrocytes as the mice mature. In the adult CNS, the highest GLAST promoter activity was observed in radial glia, such as those located in the subgranular layer of the dentate gyrus. The continued expression of GLAST by these neural progenitors raises the possibility that GLAST may have an unanticipated role in regulating their behavior. In addition, GLAST promoter activation was observed in oligodendrocytes in white matter throughout many (e.g., spinal cord and corpus callosum), but not all (e.g., cerebellum), CNS fiber tracts. Overall, these studies of GLT-1 and GLAST promoter activity, protein expression, and glutamate uptake revealed a close correlation between transgenic reporter signals and uptake capacity, indicating that these mice provide the means to monitor the expression and regulation of glutamate transporters in situ.
The therapeutic potential of cardiac μ-opioid receptors in ischaemia-reperfusion (I/R) injury during opioid-modulating diseases, such as heart failure, is unknown. We aimed to explore the changes of ...cardiac μ-opioid receptor expression during heart failure, and its role in opioid-induced cardioprotection.
Rats received doxorubicin (DOX) or were subjected to coronary artery ligation to induce heart failure, or received normal saline (NS) as control. Hearts from NS or DOX rats were isolated and subjected to myocardial ischaemia and reperfusion in an in vitro perfusion system. The opioid D-Ala,2N-MePhe,4 Gly-ol-enkephalin (DAMGO), with a high μ-opioid receptor specificity, morphine, and remifentanil were administrated before I/R with or without opioid receptor antagonists, or an extracellular signal-regulated kinase (ERK) inhibitor.
Cardiac μ-opioid receptor mRNA concentrations were 3.2 times elevated in DOX-treated rats compared with NS rats, while cardiac μ-opioid receptor protein concentrations showed 6.1- and 3.5-fold increases in DOX-treated and post-infarcted rats, respectively. DAMGO reduced I/R-caused infarct size, expressed as the ratio of area at risk, from 0.50 (0.04) to 0.25 (0.03) in failing rat hearts, but had no effect on infarct size in control hearts. DAMGO promoted phosphorylation of ERK and glycogen synthase kinase (GSK)-3β only in failing hearts. DAMGO-mediated cardioprotection was blocked by an ERK inhibitor. The μ-opioid receptor antagonist D-Pen-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr-NH2 (CTOP) prevented morphine- and remifentanil-induced cardioprotection and phosphorylation of ERK and GSK-3β in failing hearts. In contrast, δ- and κ-opioid receptor selective antagonists were less potent than CTOP in the failing hearts.
Cardiac μ-opioid receptors were substantially up-regulated during heart failure, which increased DAMGO-induced cardioprotection against I/R injury.
This experiment was designed to investigate the effects of dietary valine on the growth performance, feed utilization, digestive enzymes, serum antioxidant and immune indices of juvenile Trachinotus ...ovatus and determine its valine requirement. Six diets with different concentrations of L‐valine (15.0, 16.6, 18.6, 20.7, 23.5 and 25.4 g/kg dry diet, defined as diet Val‐1 to Val‐6.), were formulated to contain 430 g/kg crude protein with fish meal, soybean meal, peanut meal and precoated crystalline amino acids. Each diet was randomly assigned to triplicate treatments of 20 fish (the initial body weight was 5.34 ± 0.03 g) for 8 weeks. The results indicated that the final body weight and percent weight gain (PWG) increased with increasing valine concentration up to 18.6 g/kg (diet Val‐3), whereas the diets containing higher valine concentration reduced the growth performance significantly (p < .05). Moreover, the protein efficiency ratio, body protein deposition (BPD), muscle protein content, intestinal amylase and pepsin activities, serum T‐AOC, LZM activities, IgM, complement 3 and complement 4 concentration had a similar trend with PWG, and the trend of feed conversion ratio, serum AST, ALT activities, urea and MDA content was opposite. Meanwhile, the lipid contents of whole fish and muscle in diet Val‐6 were particularly lower than other diets (p < .05). The survival rate of diet Val‐1 was lowest in this study and was significantly lower than diet Val‐2 (p < .05). The results of polynomial regression based on PWG and BPD indicated that the optimal dietary valine requirement for Trachinotus ovatus reared in seawater‐floating net cages was 19.87–20.17 g/kg valine of dry diet, correspondingly 46.22–46.91 g/kg of dietary protein.
The bin packing problem is widely found in applications such as loading of tractor trailer trucks, cargo airplanes and ships, where a balanced load provides better fuel efficiency and safer ride. In ...these applications, there are often conflicting criteria to be satisfied, i.e., to minimize the bins used and to balance the load of each bin, subject to a number of practical constraints. Unlike existing studies that only consider the issue of minimum bins, a multiobjective two-dimensional mathematical model for bin packing problems with multiple constraints (MOBPP-2D) is formulated in this paper. To solve MOBPP-2D problems, a multiobjective evolutionary particle swarm optimization algorithm (MOEPSO) is proposed. Without the need of combining both objectives into a composite scalar weighting function, MOEPSO incorporates the concept of Pareto’s optimality to evolve a family of solutions along the trade-off surface. Extensive numerical investigations are performed on various test instances, and their performances are compared both quantitatively and statistically with other optimization methods to illustrate the effectiveness and efficiency of MOEPSO in solving multiobjective bin packing problems.
This study aims to explore the predictive value of T2-weighted imaging (T2WI), apparent diffusion coefficient (ADC), and early-delayed phases enhanced magnetic resonance imaging (DCE-MRI) radiomics ...prediction model in determining human epidermal growth factor receptor 2 status in breast cancer.
A retrospective study was conducted, involving 187 patients with confirmed breast cancer by postsurgical pathology at Zhenjiang First People's Hospital during January 2021 and May 2023. Immunohistochemistry or fluorescence in situ hybridization was used to determine the HER-2 status of these patients, with 48 cases classified as HER-2 positive and 139 cases as HER-2 negative. The training set was used to construct the prediction models and the validation set was used to verify the prediction models. Layers of T2WI, ADC, and early-delayed phase DCE-MRI images were used to delineate the volumeof interest and 960 radiomic features were extracted from each case using Pyradiomic. After screening and dimensionality reduction
A kinetic study of graphite oxidation provided several insights into the mechanism of graphite oxide (GO) synthesis. The oxidation was observed to occur in two distinct stages, with the first stage ...lasting for 20 to 30 min and including a rapid disruption of the graphene sp2 network, the introduction of oxygen functional groups, and an increase in the spacing between the sheets. The second stage saw a marked decrease in the rate of change in spacing, a significant increase in the homogeneity of the GO, little to no further disruption of the sp2 network, and continuing evolution of the oxygen functionality. The study was based on the analysis by Raman spectroscopy, XRD, FTIR, SEM, and TGA of material taken at various times from a modified Hummers oxidation reaction following work up.
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