Long non-coding RNAs (lncRNAs) act as important regulators of tumorigenesis and development in bladder cancer. However, the underlying molecular mechanisms remain elusive. We previously identified a ...novel lncRNA signature related to immunity and progression in bladder cancer. Here we further explored the function of RP11-89, a lncRNA discovered in the previous signature. Loss- and gain-of function experiments were performed using CCK-8 assay, flow cytometry, Transwell assays, scratch tests and subcutaneous nude mouse models. High-throughput RNA sequencing was conducted to identify dysregulated genes in bladder cancer cells with RP11-89 knockdown or overexpression. Regulation of RP11-89 on miR-129-5p and PROM2 was explored through luciferase reporter assay, RIP assay and RNA pull-down assay. RP11-89 promoted cell proliferation, migration and tumorigenesis and inhibited cell cycle arrest via the miR-129-5p/PROM2 axis. We found that RP11-89 "sponges" miR-129-5p and upregulates PROM2. Elevated PROM2 in cells was associated with attenuated ferroptosis through iron export, formation of multivesicular bodies and less mitochondrial abnormalities. We demonstrated that RP11-89 is a novel tumorigenic regulator that inhibits ferroptosis via PROM2-activated iron export. RP11-89 may serve as a potential biomarker for targeted therapy in bladder cancer.
Renal clear cell carcinoma (ccRCC) and the tumor microenvironment (TME) influence each other, leading to the tumor microenvironment that can guide the corresponding treatment. With the deepening of ...research, some treatment options have achieved good results, such as tyrosine kinase inhibitors, immune checkpoint inhibitors, and so on. As the link between TME and malignancy is constantly discovered, more targeted studies on different components of TME are increasing, and this targeted therapy is a new method for treating ccRCC, and also a current research hotspot. This review summarizes the characteristics of the ccRCC tumor microenvironment, the outcomes of different treatments, and some potential targets.
Prostate cancer (PCa) is a malignant tumor that seriously threatens men's health worldwide. Recently, stromal cells in the tumor microenvironment (TME) have been reported to contribute to the ...progression of PCa. However, the role and mechanism of how PCa cells interact with stromal cells to reshape the TME remain largely unknown. Here, using a spontaneous prostate adenocarcinoma (PRAD) model driven by the loss of Pten and Hic1, we found that M2 macrophages markedly infiltrated the stroma of Pten and Hic1 double conditional knockout (dCKO) mice compared with those in control (Ctrl) mice due to higher TGF-β levels secreted by HIC1-deleted PCa cells. Mechanistically, TGF-β in TME promoted the polarization of macrophages into "M2" status by activating the STAT3 pathway and modulating c-Myc to upregulate CXCR4 expression. Meanwhile, TGF-β activated the fibroblasts to form cancer-associated fibroblasts (CAFs) that secrete higher CXCL12 levels, which bound to its cognate receptor CXCR4 on M2 macrophages. Upon interaction with CAFs, M2 macrophages secreted more CXCL5, which promoted the epithelial-mesenchymal transition (EMT) of PCa via CXCR2. Moreover, using the TGF-β receptor I antagonist, galunisertib, significantly inhibited the tumor growth and progression of the TRAMP-C1 cell line-derived subcutaneous tumor model. Finally, we confirmed that the stromal microenvironment was shaped by TGF-β in HIC1-deficient PCa and was associated with the progression of PCa.
By using (3-2-(2-aminoethylamino)ethylaminopropyl-trimethoxysilane) and o-phenylenediamine as the reaction substrate, N-doped silicon quantum dots (N-SiQDs) with high quantum yield and excellent ...optical properties were synthesized by a one-step, simple, low-cost, and green method. Sequentially a N-SiQDs/silver nanoparticles (N-SiQDs/Ag NPs) nanocomplex was constructed as a nanoprobe to develop an "on-off" fluorescent sensing platform for hydrogen peroxide (H
2
O
2
). The produced N-SiQDs was characterized by various methods such as transmission electron microscopy, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy. Due to the surface plasmon-enhanced energy transfer from donor N-SiQDs to acceptor Ag NPs, the fluorescence of N-SiQDs is quenched ("off") by Ag NPs. In the presence of hydrogen peroxide, it etches the Ag NPs to release N-SiQDs and further increases the fluorescence intensity from N-SiQDs ("on"). Based on this principle, an "on-off" fluorescent sensing platform for hydrogen peroxide was successfully developed. Under the optimized experimental conditions, the results show that the developed sensing platform has a low detection limit (1.5 μM) and wide linear range (5.0 to 200.0 µM) for the determination of hydrogen peroxide
.
Robots that succeed in factories may struggle to complete even the simplest daily task that humans take for granted, because the change of environment makes the task exceedingly difficult. Aiming to ...teach robots to perform daily interactive manipulation in a changing environment using human demonstrations, we collected our own data of interactive manipulation. The dataset focuses on the position, orientation, force, and torque of objects manipulated in daily tasks. The dataset includes 1,603 trials of 32 types of daily motions and 1,596 trials of pouring alone, as well as helper code. We present our dataset to facilitate the research on task-oriented interactive manipulation.
We determined the conductivity, relative permeability, and thickness of homogeneous metal plates from four-point measurements of alternating current potential drop (ACPD) over the frequency range 1 ...Hz to 10 kHz. We developed simple analytical expressions from which these parameters can be calculated. In the low-frequency regime, in which the measured voltage tends to a constant, real value, the relative permeability of the plate does not influence the ACPD voltage. For known plate thickness, conductivity can be easily determined, even for ferrous metals. For known conductivity, plate thickness can be determined. Relative permeability can be obtained from measurements at higher frequency, for known conductivity and thickness. The inductance of the pickup circuit can also be determined from higher frequency measurements. This practical technique is especially useful for conductivity or plate thickness measurements in configurations where access is restricted to one side of the plate. We studied brass, aluminum, stainless steel, spring steel, and carbon steel plates. Our results agree with available independent measurements.
This study was to investigate guiding role of elevated pretreatment serum carcinoembryonic antigen (CEA) levels for ACT receipt in stage IIA colon cancer.
Eligible patients diagnosed with stage IIA ...colon cancer (
= 21848) were identified from the Surveillance, Epidemiology, and End Results (SEER) database between January 2004 and December 2010. Pearson's chi-squared tests, Cox proportional hazards regression models, and Kaplan-Meier methods were performed. Propensity score matching (PSM) was used to decrease the risk of biased estimates of treatment effect.
Multivariate Cox analysis indicated that, in CEA-elevated group, receiving or not receiving ACT did not presented statistically CSS difference hazard ratio (HR) = 0.940, 95% confidence interval (CI) = 0.804-1.097,
= 0.431; in CEA-normal group, receiving or not receiving ACT also did not presented statistically CSS difference (HR = 0.911, 95% CI = 0.779-1.064,
= 0.239). After PSM, Kaplan-Meier analyses showed that there was no statistical CSS difference between receiving or not receiving ACT (
= 0.64).
ACT did not show substantial survival benefit in stage IIA colon cancer with elevated pretreatment serum CEA levels. Stage IIA disease with elevated pretreatment serum CEA should not be treated with ACT.
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