•Staphylococcus pseudintermedius is a commensal bacterium residing on the mucosae and skin surfaces of dogs.•S pseudintermedius has emerged as a global concern in small animal practices.•This report ...describes pyometra associated with S pseudintermedius in two bitches.•In one bitch the infection was lethal due to septic shock.•In the other bitch the bacteria caused pyometra with suppurative peritonitis.
Staphylococcus pseudintermedius is a commensal bacterium residing on the mucosae and skin surfaces of dogs. Under favorable conditions, the organism may cause serious infections, primarily involving the skin, but it is also isolated in eye and ear infections. This report describes pathologic findings of pyometra associated with S pseudintermedius in two bitches.
One bitch was a 6-year-old Pomeranian bitch who died after three days of diarrhea, vomiting, and depression. During necropsy, the uterus of the bitch was diffusely enlarged and filled with 30 mL of thick, yellow, creamy pus. Histology revealed acute to subacute, diffuse, severe, purulent endometritis with intralesional Gram-positive round cocci revealed as S pseudintermedius. The cause of death was pyometra with subsequent septic shock.
The other bitch was a 2-year-old West Highland White Terrier who underwent diagnostic laparotomy due to suppurative peritonitis of unknown cause. During the procedure, about 100 mL of yellow, opaque exudate from the abdominal cavity was removed. The distended uterine horns noted during the procedure were removed and submitted for histopathology which revealed necrosis of endometrium with the accumulation of pus and numerous, intralesional S pseudintermedius in the uterine lumen. Focally, uterine wall necrosis was evident, with a spread of bacteria and purulent inflammation into the adjacent mesometrium causing purulent peritonitis.
Although S pseudintermedius presents a common cause of skin and mucosal infection, it should be considered a possible etiological agent of canine pyometra.
Endometrial adenocarcinomas present rare neoplasia of bitches. This case report describes mucinous endometrial adenocarcinoma in a bitch with concurrent pyometra for the first time. A mass on the ...uterine stump was removed during surgery of a 13-year-old bitch with clinically suspected pyometra. Histopathology revealed mucinous endometrial adenocarcinoma. The tumor was classified according to human classification, responding to women's type I (endometrioid carcinoma). Immunohistochemistry showed a positive expression of estrogen receptor α, progesterone receptor, p53, and p16 gene, while vimentin was not expressed.
Species of the genus Hepatozoon (Adeleorina: Hepatozoidae) are arthropod-transmitted protozoan parasites that infect a wide range of vertebrate hosts. In the present study, we describe a new species ...of Hepatozoon primarily infecting martens and propose the name Hepatozoon martis n. sp., based on its unique morphological, molecular and pathogenic features. The overall prevalence of infection with H. martis n. sp. assessed by PCR in European pine martens (Martes martes) from Bosnia and Herzegovina and stone martens (Martes foina) from Croatia was 100% and 64%, respectively. Gamonts were found in neutrophils and monocytes, and various developmental stages were described in tissue cross-sections. Hepatozoon martis n. sp. shows a high predilection for muscle tissue, and the heart was the most frequently affected organ among the tissues tested by histopathology. Microscopically, pyogranulomatous lesions associated with the presence of the parasitic forms were observed in the cardiac and skeletal muscles of all positive animals examined. Furthermore, the possible existence of alternative, non-vectorial routes of transmission is discussed.
Cytology is the diagnostic procedure of the microscopic evaluation of cells. It is becoming increasingly important and more frequently used in veterinary diagnostics, having many advantages including ...simplicity, speed and low cost. To determine the pathological changes diagnosed by cytology in cats, as well as the distribution of age, breed and gender in the diagnosed changes, we performed a retrospective study on slides submitted to the Department of Veterinary Pathology for routine cytological examination. The archive was searched for all feline cytology slides submitted from 2009 to 2018. All the slides were re-evaluated and classified into one of the four pathological processes: ‘neoplasia’, ‘inflammation’, ‘other condition’ or ‘non-diagnostic sample’. Breed, age, gender and the tissue from which the lesion was sampled were noted from the submission form, and statistically analyzed. The most frequent type of pathological process diagnosed was neoplasia, which reflects the high prevalence of neoplastic diseases in cats reported in literature data. Pathological changes were mostly diagnosed in domestic shorthaired cats of both sexes, with an average age of 8.4 years, but no breed, age or gender predisposition was found. The most evaluated tissue was skin, probably due to its accessibility and the ease of obtaining a sample from skin lesions. The most frequent neoplasia were malignant and the most frequent diagnosis was round cell neoplasia. Cats affected with round cell neoplasia had a significantly lower average age (7.3 years) than cats diagnosed with epithelial and mesenchymal neoplasia (9.9 and 10.3 years, respectively), probably reflecting the common retroviral infection in Croatian cats.
Visceral hemangiosarcoma were analyzed at the Department of Veterinary Pathology of the Faculty of Veterinary Medicine, University of Zagreb, over a 5-year period. From a total of 52 tumor masses in ...a total of 31 dogs, histological growth patterns (cavernous, capillary or solid) and the amount of tumor supporting stroma were evaluated. Additionally, sections were stained with toluidine blue for the purpose of mast cell detection and their number was determined in the tumor parenchyma, tumor stroma and edges. The average age of the affected animals was 10 years, males predominated, and the tumors occurred most frequently in cross breeds, German Shepherd dogs and Labrador Retrievers. The highest number of visceral hemangiosarcoma was found in the spleen (27/52). The most common growth pattern of visceral hemangiosarcoma was solely cavernous with a mixture of cavernous and solid patterns in different proportions. Mast cells were found in 44/52 (84.6%) of the visceral hemangiosarcomas. A smaller number of mast cells were found in the tumor parenchyma, and higher number were found in the stroma and tumor edges. The number of mast cells in tumors was not significantly associated with the tumor growth pattern, but there was a positive correlation between MCC - tumor parenchyma and stroma (rs = 0.28, P<0.05), MCC - tumor stroma + edges (rs = 0.74, P<0.05) and MCC - tumor parenchyma and MCC- tumor stroma+edges (rs = 0.30, P<0.05) in all the examined tumors. In splenic tumors, there was only a significant positive correlation between MCC - stroma+edges (rs P = 0.68 P<0.05). These results suggest a higher mast cell count in tumors with more developed stromal components in canine visceral hemangiosarcomas, and certainly indicate the need for further research on their role and the factors they release in the development and progression of hemangiosarcomas.
Canine follicular tumours (CFT) are an uncommon type of keratin-producing tumours in dogs that develop from different structures of the hair follicle. CFT are classified as infundibular keratinizing ...acanthoma (IKA), tricholemmoma, trichoepithelioma, pilomatricoma and trichoblastoma. Studies on CFT are rare and most descriptions of these tumours are presented as case reports. Thus, we performed a retrospective study to investigate the prevalence and types of CFT within the Croatian canine population. The study included all CFTs presented to the Department of Veterinary Pathology in a 4 year period (2009 to 2012). In the investigated period, 714 skin tumours were diagnosed, from which only 69 presented as CFT: 10 IKA, 21 pilomatricoma, 1 malignant pilomatricoma, 12 trichoepithelioma, 4 malignant trichoepithelioma and 21 trichoblastoma. No breed or gender predisposition was found. However, pilomatricoma was mostly diagnosed in breeds with continuous hair growth, while IKAs were mostly found in breeds without continuous phase hair growth coats. Tumours were mostly presented as nodules and rarely as warts or plaques, growing in the dorsal aspect of the skin on the head, back and hind legs. CFT still represent a small number of tumours compared to other types of neoplasia diagnosed in Croatian canine patients. In the majority of cases, these tumours consist of a benign proliferation. However, in 7.2% of CFT an infiltrative malignant form was diagnosed. Keratin-filled skin nodules are therefore required for histopathological evaluation to avoid the unfavorable consequences of malignant spread. Key words: dog, pilomatricoma, trichoepithelioma, trichoblastoma, IKA, prevalence in Croatia Folikularni tumori pasa (FTP) pripadaju skupini rijetkih tumora koze koji stvaraju keratin, a razvijaju se iz razlicitih dijelova dlacnog folikula. Skupina psecih folikularnih tumora sastoji se od infundibularnog keratinizirajuceg akantoma (IKA), triholemoma, trihoepitelioma, pilomatrikoma i trihoblastoma. Sustavna istrazivanja FTP-a su rijetka, a vecina publiciranih opisa ovih tumora odnosi se na prikaze klinicke i histoloske slike pojedinacnih slucajeva. S ciljem da odredimo prevalenciju i tipove FTP-a u populaciji pasa u Hrvatskoj proveli smo cetverogodisnje retrospektivno istrazivanje na uzorcima FTP-e koji su obradeni u Zavodu za veterinarsku patologiju od 2009. do 2012. godine. U istrazivanom razdoblju dijagnosticirano je 714 tumora koze, od kojih je samo 69 tumora svrstano u FTP: 10 IKA, 21 pilomatrikom, 1 maligni pilomatrikom, 12 trihoepitelioma, 4 maligna trihoepitelioma i 21 trihoblastom. Pasminska i spolna predispozicija nisu utvrdene, ali je uoceno da pilomatrikomi u najvecem broju slucajeva nastaju u pasmina pasa s kontinuiranim rastom dlake, dok je IKA-e vecinom dokazana u pasmina koji nemaju tu karakteristiku rasta dlacnog pokrova. FTP se najcesce ocituju kao noduli, rijetko kao bradavice ili plakovi, te su vecinom zahvacali dorzalno podrucje koze na glavi i ledima te proksimalno podrucje na straznjim nogama. Usporedno s ostalim tipovima neoplazija dijagnosticiranih u hrvatskih pasa FTP predstavljaju rijetkost. Iako dominantno pokazuju biolosko ponasanje tipicno za benigne proliferacije, u ovom istrazivanju je u 7,2% slucajeva FTP-a dijagnosticirana infiltrativna, maligna varijanta. Kako bi se izbjegle nezeljene posljedice sirenja malignog procesa, sve kozne nodule ispunjene keratinom potrebno je histoloski klasificirati. Kljucne rijeci: pas, pilomatrikom, trihoepiteliom, trihoblastom, akantom, prevalencija, Hrvatska
The study aimed to determine reproductive span by investigation of the ovarian structures in young and elderly captive brown bear females (Ursus arctos). The ovaries of two 2-year-old females were ...obtained by ovariectomy and during the necropsies of 31 and 36 year old individuals. All the obtained ovaries were examined macroscopically and histologically. Histological examination of the ovaries of young animals (2+ years) revealed the presence of primordial, primary, secondary and tertiary follicles within the ovarian cortex. One ovary showed a mature corpus luteum, indicating recent ovulation, what is, to our knowledge, the first histological proof of the earliest age of ovulation recorded for captive brown bears. Ovarian atrophy accompanied by the development of multiple cystic subsurface epithelial structures (SES) in the case of the old bears in this study indicates that the ovaries of brown bears share similar degenerative and proliferative patterns with domestic canids. The oldest female had records of successful births at the ages of 26 and 28 years. Both recorded birth events represent one of the latest confirmed occurrences of ovulation, conception and birth amongst brown bears. Key words : reproduction; puberty; ovulation; corpus luteum; post-reproductive phase
Mastocitomi su česti kožni tumori. Intestinalni su mastocitomi međutim rijetki u pasa te su najčešće opisani u stijenci želuca. Ovaj prikaz slučaja opisuje rijedak slučaj primarnog intestinalnog ...mastocitoma unutar rektuma kuje stare pet godina, koja nije pokazivala konkurentne kožne tvorbe
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