Aim
Disruption of the intestinal mucosal tolerance, that is, the immunological unresponsiveness to innocuous food antigens and the commensal microbiota, in the colon is associated with several ...chronic diseases including inflammatory bowel disease (IBD). Understanding the mechanisms responsible for intestinal mucosal tolerance has potential translational value for its therapy and management. Human intestinal mesenchymal cells (iMCs) play important roles in colonic mucosal tolerance, but further studies on their tissue regenerative and immunomodulatory capacities are necessary in order to fully understand their function in health and disease.
Methods
In this study, we have isolated and analysed the capacity of human iMCs to promote wound healing and modulate immune responses in vitro and in vivo, using the dextran sulfate sodium (DSS)‐induced colitis model.
Results
Cultured iMCs were CD45−CD73+CD90+CD105+ and accelerated the wound closure in a normal colon mucosa (NCM) 356 human epithelial cell wound healing assay. Furthermore, iMCs blocked the LPS‐mediated induction of TNF‐α in THP‐1 macrophages and inhibited the proliferation of peripheral blood mononuclear cells, partly through the induction of indoleamine‐2,3‐dioxygenase. In DSS colitic mice, iMCs administration reduced the disease activity index and ameliorated intestinal tissue damage and permeability. Furthermore, iMCs reduced intestinal inflammation, evidenced by a decreased mRNA expression of pro‐inflammatory cytokines, reduced IL‐1β secretion by intestinal explants and inhibited colonic iNOS protein expression.
Conclusions
Our data show that human iMCs isolated from the noninflamed intestine possess tissue‐regenerative and immunomodulatory capacities that could potentially be harnessed/restored in order to reduce IBD severity.
This text aims to identify the theories of property rights
that are involved in the Constitutional Court and the
Supreme Court rulings on the issue of the allocation
of private property rights on ...vacant lots showing their
tensions and recent interactions. Then, the theories of
utilitarianism and Locke’s theories initially explained
are associated with the arguments reviewed in
17 judicial decisions of the Constitutional Court
and the Supreme Court referred to writs of amparo
between July 2014 and October 2016. The review
shows the arguments of the Constitutional Court
with the utilitarian theory that was supported by the
Supreme Court, except in early 2016 when notions of
Locke’s theory about property rights were accepted.
In addition, attempts to abstract the discussion on
vacant lots from the constitutional sphere to move it
to the usual civil law scenario are being made.
Este texto busca identificar las teorías
sobre los derechos de propiedad que
están involucradas en las providencias
de las Cortes Constitucional y Suprema
de Justicia en el tema de la asignación
de derechos de propiedad sobre predios
baldíos, evidenciando sus tensiones e
interacciones recientes. Entonces, se
asocian las teorías del utilitarismo y de
Locke explicadas en un primer momento,
con los argumentos revisados en 17
providencias de las Cortes referidas a
acciones de tutela que pretenden revisar
procesos de prescripción del dominio
entre julio de 2014 y octubre de 2016.
La revisión muestra los argumentos de
la Corte Constitucional con la teoría
utilitarista que fue respaldada por la
Corte Suprema, excepto a inicios de
2016 cuando se acogieron nociones de
la teoría de Locke sobre los derechos
de propiedad. Además, se muestran
intentos de abstraer la discusión sobre
baldíos del ámbito constitucional para
trasladarla al habitual escenario del
derecho civil.
This text aims to identify the theories of property rights that are involved in the Constitutional Court and the Supreme Court rulings on the issue of the allocation of private property rights on ...vacant lots showing their tensions and recent interactions. Then, the theories of utilitarianism and Locke's theories initially explained are associated with the arguments reviewed in 17 judicial decisions of the Constitutional Court and the Supreme Court referred to writs of amparo between July 2014 and October 2016. The review shows the arguments of the Constitutional Court with the utilitarian theory that was supported by the Supreme Court, except in early 2016 when notions of Locke's theory about property rights were accepted. In addition, attempts to abstract the discussion on vacant lots from the constitutional sphere to move it to the usual civil law scenario are being made.
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