Persicaria amphibia (subfam. Polygonoideae), an aquatic macrophyte rich in
dietary polyphenolics, is used as a traditional remedy and culinary herb.
Nevertheless, P. amphibia from the Balkan region ...has been insufficiently
studied and underutilized. Here, the cytotoxicity and antimicrobial
properties of the previously chemically characterised ethanol extract of P.
amphibia aerial parts were tested. The following methods were carried out:
the MTT assay, qRT-PCR, microdilution assay, Chromobacterium violaceum
screening assay (monitoring of quorum sensing, QS) and the agar plating
method (antifungal activity). The study was conducted to determine the
cytotoxic effects of P. amphibia against lung cancer cells (A549) and its
combination with cytostatic doxorubicin (Dox). A dose-dependent decrease in
cell viability (up to 82% reduction) and additive interactions of the tested
agents were noted. Both alone and combined with Dox, P. amphibia reduced the
expression of Nrf2 (p < 0.05). In terms of antimicrobial effects, P.
amphibia exhibited an antipathogenic effect since it disrupted QS
communication, which was evident through the inhibition of violacein
production of C. violaceum CV025. The antifungal screening revealed that P.
amphibia induced significant growth inhibition of Aspergillus spp. (28.23%).
Based on the obtained results, further examination of the potential use of
P. amphibia in modern phytotherapy and diet-derived cancer chemoprevention
is encouraged.
Background/Aim. Reactive thrombocytosis, as a paraneoplastic syndrome, is often observed in cancer patients. A variety of tumor-related humoral factors and cytokines con-tribute to tumor-stimulated ...thrombopoiesis. However, the exact role of these cytokines in the pathogenesis of thrombocytosis remains unclear. The aim of this study was to analyze systemic values of cytokines and clinical-pathological characteristics in colorectal carcinoma (CRC) patients with and without thrombocytosis. Methods. Fifty nine CRC patients were involved in this study and divided into two groups according to the number of platelets. We recorded and analyzed the data about: age, gender, size of the cancer, localization, metastasis, vascular or lymph vessel invasion, nuclear grade, histological differentiation rate, tumor, nodus, metastasis (TNM) stage and concentration of cytokines interleukin (IL)-1, IL-33, IL-12, IL-17 and interferon (IFN)-? in both groups. Results. CRC patients with thrombocytosis had significantly higher nuclear grade of the cancer (p = 0.002); higher percentage of detectable metastatic lesions in the liver (p = 0.002), lung (p = 0.001), peritoneal carcinomatosis (p = 0.001), detectable invasion of blood (p = 0.012) and lymph vessels (p = 0.010). Concentrations of tumor markers alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and cancer antigen 19-9 (CA19-9) and se-rum values of IL-1 a nd I L-33 were significantly higher in CRC patients with thrombocytosis. IL-1/IL-12 (p = 0.016), IL-1/IFN-? (p = 0.007), IL-1/IL-17 (p = 0.006), IL-33/IL- 12 (p = 0.001), IL-33/IFN-? (p = 0.001), IL-33/IL-17 (p = 0.002), and IL-33/IL-1 (p = 0.006) ratios were significantly higher in CRC patients with thrombocytosis in comparison to CRC patients without thrombocytosis. Analysis of Receiver Operating Characteristic (ROC) curves showed that values of IL-1 area under curve (AUC) = 0.718; 95% confidence interval (CI): 0.567?0.868; sensitivity 69.2%, specificity 62.9% and IL-33 (AUC = 0.763; 95% CI: 0.614? 0.911; sensitivity 84.6%, specificity 65.7%), could be serve as possible markers for paraneoplastic thrombocytosis in CRC patients. Conclusion. IL-1 a nd I L-33 significantly correlated to high thrombocyte number in patients with more aggressive CRC.
Following the idea of sustainability in food production, a yogurt premix based on beetroot (
) pomace flour (BPF) was developed. BPF was granulated with lactose solution containing lactic acid ...bacteria (LAB) by a fluidized bed. Particle size increased ~30%. A decrease in Carr Index from 21.5 to 14.98 and Hausner ratio from 1.27 to 1.18 confirmed improved flowability of granulated BPF, whereas a decrease in water activity implied better storability. Yogurts were produced weekly from neat starters and granulated BPF (3%
/
) that were stored for up to one month (4 °C). High viability of
was observed. Less pronounced syneresis, higher inhibition of colon cancer cell viability (13.0-24.5%), and anti-
activity were ascribed to BPF yogurts or their supernatants (i.e., extracted whey). Acceptable palatability for humans and dogs was demonstrated. A survey revealed positive consumers' attitudes toward the granulated BPF as a premix for yogurts amended to humans and dogs. For the first time, BPF granulated with LAB was used as a premix for a fermented beverage. An initial step in the conceptualization of a novel DIY (do it yourself) formula for obtaining a fresh yogurt fortified with natural dietary fiber and antioxidants has been accomplished.
The existing building stock energy consumption accounts for about 38% of final energy consumption in Republic of Serbia. 70% of that energy is consumed by residential sector, mostly for space ...heating. This research is addressed to the single family house building placed in the Belgrade city. The house has ground and first floor with total heating area of 130 m2 and pellet as space heating source. The aim of this paper is to evaluate energy and economic analysis for different heating systems. Several homeheating were compared: Option 1 (biomass combustion boiler using pellet as a fuel), Option 2 (gas combustion boiler) and Option 3 (heat pump). The building performance was evaluated by TRNSYS 17 simulation code. Results show estimated savings using renewable energy sources. Projekat Ministarstva nauke Republike Srbije, br. III42008
The paper analyses the influence of cigarette butts and waste coffee grounds
addition on the properties of the brick clay. The waste materials were
added to the clay in amounts of 5 wt.% and 10 wt.%. ...Standard consistency,
plasticity, drying and firing behaviour and refractoriness were tested on the
clay sample and the samples with wastes additions. Apparent density, apparent
porosity, water absorption, strength and thermal conductivity were investigated
on the samples fired at 1173 K. Addition of the waste materials improved thermal insulation characteristics and drying shrinkage, while other properties remain within the required limits for brick industry
Laboratory-isolated essential oils are commonly used in experiments regarding their utilization as natural antioxidant and antimicrobial agents. This study analyzed commercially available essential ...oils (Serbian-FSR and Russian-FRU) of fennel (Foeniculum vulgare Mill.) and added to the pork patties to investigate their possible role as natural antioxidant and antimicrobial agents. Chemical analysis of the samples showed significant differences in profiles caused by geographical origin and other environmental factors. The main compound in both samples was trans-Anethole, but it had a much higher content in Serbian oils. On the contrary, Russian oil had a high portion of anisaldehyde and linalool. These diversities are the main reason for thermal behavior and biological activity differences. The thermal analysis has shown that FSR had a crystalline form in the solid state, while FRU had an amorphous form. During heating, in FSR, a single-step weight loss was detected, while in FRU, a two-step weight loss was observed, indicating that, in addition to evaporation, components in FRU interacted with each other, forming lesser volatile molecules. After adding pork patties, Russian oil showed higher potency and better protective behavior than Serbian oil. Moreover, mixtures of these oils were added to the patties samples, and results showed higher potency when compared to individual oil samples. This indicated that minor compounds from both oils interacted and synergistically acted to prolong the shelf life of the meat samples. Commercial essential oils could be successfully utilized to prolong shelf life and provide additional intake of natural compounds significant for the human diet.
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•Fennel's essential oil from Serbia (FSR) and Russia (FRU) (EO) were analyzed.•Serbian oil had a crystalline form, while Russian had an amorphous form.•Biological activity assays showed that FRU was more potent than FSR.•Both oils successfully prolonged shelf life of pork patties, but FRU was more potent.•FRU and FSR mixtures were more potent comparing to the individual oils.
To the best of the author’s knowledge, so far, the utilization of industrial solid
Ganoderma lucidum
waste was not investigated as a potential source of remaining valuable bioactive compounds. The ...aim of our study was to chemically characterize the extract prepared with 96% and 70% ethanol utilizing
G. lucidum
waste from industrial ethanol (GE) and water (GW) extraction. The phenolic profile was assessed by UHPLC-MS/MS, while the amino acid content of selected extracts was done by GC-MS. The chemical composition of the extracts was obtained by ATP-FTIR analysis, and the peak at 1637 cm
− 1
of spectra originating from C = O stretching vibration of amide I, was exclusively detected in samples prepared with 70% ethanol. Among eleven amino acids detected in
G. lucidum
residues, the most abundant was essential amino acid phenylalanine. Phenolic profile revealed
p
-hydroxybenzoic acid and chlorogenic acid as dominant in all the samples analyzed. The cytotoxic activity against two human cancer cell lines (colon carcinoma (HCT116) and melanoma (Hs294T)), photoprotective activity, and antioxidant activity of
G. lucidum
residues ethanol extracts (70% and 96%) were examined. The most prominent cytotoxic effect on HCT116 and Hs294T cells was attributed to extract prepared from the water extract production. The sun protection factor (SPF) values of extracts analyzed were found to be in the range from 1.86 (GE96) to 4.80 (GW70). Therefore,
G. lucidum
solid waste appeared to be a valuable source of bioactive compounds with antioxidant, photoprotective and cytotoxic activities.
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