Intravenous inoculation of young rabbits with 1.4 x 10(5) spores ml-1 of Absidia corymbifera produced gross and microscopic changes principally in the kidneys. Grossly, there was congestion, ...haemorrhages and greyish-white necrotic foci giving a mottled appearance to the kidneys. Microscopically, besides congestion and haemorrhages, there was a granulomatous reaction characterized by a central necrotic mass containing mycelium surrounded by a reactive zone of neutrophils, lymphocytes, a few macrophages and giant cells. Fibrous tissue proliferation was also present. Characteristic dichotomously branched non-septate fungal hyphae could be demonstrated in the sections of kidneys up to 14 days of infection. In one rabbit, which had died on the 8th day, small granulomatous lesions with fungal hyphae were noticed in the liver's parenchyma.
Absidiosis was produced experimentally in rabbits by intravenous inoculation of 1.4 x 10(5) spores of Absidia corymbifera. Infected rabbits exhibited a rise in body temperature, anorexia, dullness, ...listlessness, diarrhoea, occasional blindness, convulsions and death in some cases. Mortality occurred mainly between 6 to 9 days post infection (DPI) and overall mortality was 50 per cent during the three week observation period. No significant difference was observed in erythrocytic indices viz., Hb, PCV, TEC in control and infected rabbits. However, erythrocyte sedimentation rate was considerably increased in the infected rabbits. A state of leucocytosis was observed in the infected rabbits, which was due to increase in the relative percentage of neutrophils and decrease in lymphocytes. There was a significant increase in blood urea nitrogen concentrations of infected rabbits from 3 to 14 DPI as compared to controls, but serum creatinine values were not significantly altered at any stage of infection. The cause of death was attributed to kidney failure and uraemia in infected rabbits. The rabbit was found to be a suitable model for the study of absidiosis.
AstroSat is a multi-wavelength satellite launched on 2015 September 28. The CZT Imager of AstroSat on its very first day of operation detected a long duration gamma-ray burst (GRB) namely GRB ...151006A. Using the off-axis imaging and spectral response of the instrument, we demonstrate that CZT Imager can localise this GRB correct to about a few degrees and it can provide, in conjunction with Swift, spectral parameters similar to that obtained from Fermi/GBM. Hence CZT Imager would be a useful addition to the currently operating GRB instruments (Swift and Fermi). Specifically, we argue that the CZT Imager will be most useful for the short hard GRBs by providing localisation for those detected by Fermi and spectral information for those detected only by Swift. We also provide preliminary results on a new exciting capability of this instrument: CZT Imager is able to identify Compton scattered events thereby providing polarisation information for bright GRBs. GRB 151006A, in spite of being relatively faint, shows hints of a polarisation signal at 100-300 keV (though at a low significance level). We point out that CZT Imager should provide significant time resolved polarisation measurements for GRBs that have fluence 3 times higher than that of GRB 151006A. We estimate that the number of such bright GRBs detectable by CZT Imager is 5 - 6 per year. CZT Imager can also act as a good hard X-ray monitoring device for possible electromagnetic counterparts of Gravitational Wave events.
Counter-immunoelectrophoresis was employed for the detection of Brucella antigen in stomach contents of aborted buffalo fetuses and antibody in aborted as well as apparently healthy in contact ...buffaloes. Five of 16 aborted cases were serologically positive for brucellosis but isolation of Brucella abortus was successful in only two cases. By counter-immunoelectrophoresis, Brucella antigen was detected in the fetal stomach contents of four serologically positive cases. Of the 68 serum samples from in contact healthy buffaloes, 10 were positive with counter-immunoelectrophoresis: more than were detected by tube agglutination, Rose Bengal plate agglutination, complement fixation and agar gel precipitation test.
Among 21 fetuses from serologically positive dams soluble brucella antigen was detected in the stomach contents in 14 instances by agar gel precipitation test and in 19 by ...counterimmunoelectrophoresis. Brucella abortus biotype 3 was isolated from the fetal stomach contents in 15 of these cases. None of 20 fetuses from serologically negative dams revealed brucella antigen in the stomach contents in either test.
A dot Enzyme-linked Immunosorbent Assay (dot-ELISA), using whole cell Brucella abortus antigen dotted on the nitrocellulose membrane bound to a plastic strip (dipstick) was employed for the detection ...of Brucella antibodies in bovine sera. The results were compared with that of serum agglutination (SAT), Rose Bengal plate agglutination (RBPT) and Complement Fixation test (CFT). All the four tests gave negative reaction in 127 sera obtained from a brucellosis free herd. Testing of 549 sera from a chronically infected herd revealed 57 positive and 447 negative animals in all the four assays. Of the remaining 45 sera, 34 were positive in dot-ELISA. Six of these cases were independently detected by dot-ELISA while 28 showed positive reactions in combination with other tests. When serum samples from 158 aborted cases were subjected to dot-ELISA, 79 were found positive. Of these dot-ELISA positive cases, 71 gave positive reaction in SAT, 72 in RBPT and 78 in CFT. B. abortus biotype 3 was isolated from 34 of the 98 aborted fetuses examined.
Foetal birthweight, placental morphometry and maternal, cord blood and placental haemoglobin and iron levels were studied in 69 anaemic mothers (haemoglobin <110 g/1) and 16 mothers without anaemia ...(haemoglobin ≥110 g/1). The birthweight, placental weight and number of placental cotyledons were significantly reduced in the severely anaemic mothers (haemoglobin ≤60 g/1) and had direct relationships with the maternal haemoglobin levels. However, placental volume and surface area showed no constant relation to maternal haemoglobin. The haemoglobin and iron levels in the cord blood and placental tissue were found to have linear correlations with the maternal haemoglobin levels. The low levels of placental and cord serum iron in the severely anaemic mothers suggest that iron supply to the placenta and the foetus is affected in maternal anaemia and the foetus takes iron in direct proportion to the levels available in the mother.
A case of hydronephrosis in ovine kidney Khanna, R.N.S; Chand, P. (Haryana Agricultural University, Hisar (India). Department of Veterinary Pathology)
Haryana Agricultural University Journal of Research (India),
(Dec 1993), Letnik:
23, Številka:
4
Journal Article
